Surface acoustic wave (SAW) sensors in tunable oscillator configuration, with a deposited polymeric layer, were used to investigate the layer’s impact on the oscillator’s resonant frequency. The SAW oscillators were tuned by means of variable loop amplification. Full-range amplification variation led to a resonant frequency increase of ~1.7 MHz due to the layer’s nonlinear reaction. The layer’s morphology and location resulted in a specific resonant frequency–amplitude dependence. Five types of layers were used to test the causal linkage between the layers’ morphological parameters or positioning and the SAW oscillator’s resonant frequency. The frequency variation trend is almost linear, with a complex minute variation. Small amplitude sigmoids occur at certain attenuation values, due to layer acoustic resonances. Multiple sigmoids were linked with layer resonances of different orders. A good correlation between the layer’s thickness and resonance position was found.