Design, Synthesis, Characterization and Toxicity Studies of Poly (N-Iso- Propylacrylamide-co-Lucifer Yellow) Particles for Drug Delivery Applications

Mohsen, Reham; BD, Alexander

doi:10.4172/2157-7439.1000363

Cited by 6 publications

(4 citation statements)

References 51 publications

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“…This brought a diverse cell internalization between tumor and normal cells as suggested by flow cytometry analysis, and therefore, to a distinct cytotoxic effect. It is worth mentioning that the biocompatibility of p(NIPAM) was previously tested by Mohsen et al [ 41 ] when it showed cell viability over 90% at concentrations up to 3 mg/mL.…”

Section: Resultsmentioning

confidence: 99%

“…A Surfactant Free Emulsion Polymerisation (SFEP) technique was used for the preparation of p(NIPAM)-co-5%AA as described previously and in accordance with literature [ 27 , 28 , 29 , 41 ]. Briefly, a three-neck lid was then fitted to the reaction vessel, which was placed onto a hot plate stirrer and heated to 70 °C with continuous stirring under N2 atmosphere.…”

Section: Methodsmentioning

confidence: 99%

“…Gels 2021, 7, x FOR PEER REVIEW 12 of 18 cytotoxic effect. It is worth mentioning that the biocompatibility of p(NIPAM) was previously tested by Mohsen et al[41] when it showed cell viability over 90% at concentrations up to 3 mg/mL.…”

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confidence: 99%

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Functionalized Poly(N-isopropylacrylamide)-Based Microgels in Tumor Targeting and Drug Delivery

Campora

Mohsen

Passaro

et al. 2021

Gels

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Over the past several decades, the development of engineered small particles as targeted and drug delivery systems (TDDS) has received great attention thanks to the possibility to overcome the limitations of classical cancer chemotherapy, including targeting incapability, nonspecific action and, consequently, systemic toxicity. Thus, this research aims at using a novel design of Poly(N-isopropylacrylamide) p(NIPAM)-based microgels to specifically target cancer cells and avoid the healthy ones, which is expected to decrease or eliminate the side effects of chemotherapeutic drugs. Smart NIPAM-based microgels were functionalized with acrylic acid and coupled to folic acid (FA), targeting the folate receptors overexpressed by cancer cells and to the chemotherapeutic drug doxorubicin (Dox). The successful conjugation of FA and Dox was demonstrated by dynamic light scattering (DLS), Fourier-transform infrared (FTIR) spectroscopy, thermogravimetric analysis (TGA), UV-VIS analysis, and differential scanning calorimetry (DSC). Furthermore, viability assay performed on cancer and healthy breast cells, suggested the microgels’ biocompatibility and the cytotoxic effect of the conjugated drug. On the other hand, the specific tumor targeting of synthetized microgels was demonstrated by a co-cultured (healthy and cancer cells) assay monitored using confocal microscopy and flow cytometry. Results suggest successful targeting of cancer cells and drug release. These data support the use of pNIPAM-based microgels as good candidates as TDDS.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Functionalized Poly(N-isopropylacrylamide)-Based Microgels in Tumor Targeting and Drug Delivery

Campora

Mohsen

Passaro

et al. 2021

Gels

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“…At pH values lower than pKa, the acrylic acid carboxyl groups are protonated (COOH) and the hydrogel is contracted. At lower pH values, the amino groups in the NIPAM monomer are protonated (NH 2 + ) and electrostatic repulsive forces increase the hydrophilicity of the polymeric network and hydrogel swells [26]. Hydrophilic acrylic acid contributes to a higher degree of water absorption and to a higher value of lower critical solution temperature, which is close to the physiological body temperature [27].…”

Section: Introductionmentioning

confidence: 99%

Modified Biochanin A Release from Dual pH- and Thermo-Responsive Copolymer Hydrogels

et al. 2021

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The temperature- and pH-responsive poly(N-isopropylacrylamide-co-acrylic acid), p(NIPAM-co-AA), copolymer was synthesized by free radical polymerization and examined as a carrier for modified release of biochanin A. Biochanin A is a biologically active methoxylated isoflavone which exhibits estrogenic and other pharmacological activities. Due to its poor aqueous solubility and extensive first-pass metabolism, biochanin A has low bioavailability. The aim of this work was to incorporate biochanin A into the synthesized p(NIPAM-co-AA) copolymer and to examine its release at the body temperature and pH values that correspond to pH values of vaginal and rectal cavities. The amount of released biochanin A was monitored by the ultra-visible spectroscopy (UV-Vis) method. The structure of synthesized p(NIPAM-co-AA) copolymer and copolymer with incorporated biochanin A were characterized by using Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) methods. The content of residual monomers in the synthesized copolymer was analyzed by using the high-pressure liquid chromatography (HPLC) method. The swelling behavior of p(NIPAM-co-AA) copolymer was monitored in relation to the temperature and pH values of the surrounding medium. For modelling the process of p(NIPAM-co-AA) copolymer swelling, the full three-level factorial design was applied.

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Synthesis, characterization and in vitro cytotoxicity studies of poly-N-isopropyl acrylamide gel nanoparticles and films

Litowczenko

Gapiński

Markiewicz

et al. 2021

Materials Science and Engineering: C

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Design, Synthesis, Characterization and Toxicity Studies of Poly (N-Iso- Propylacrylamide-co-Lucifer Yellow) Particles for Drug Delivery Applications

Cited by 6 publications

References 51 publications

Functionalized Poly(N-isopropylacrylamide)-Based Microgels in Tumor Targeting and Drug Delivery

Functionalized Poly(N-isopropylacrylamide)-Based Microgels in Tumor Targeting and Drug Delivery

Modified Biochanin A Release from Dual pH- and Thermo-Responsive Copolymer Hydrogels

Synthesis, characterization and in vitro cytotoxicity studies of poly-N-isopropyl acrylamide gel nanoparticles and films

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