Abstract. Sustainable development is a big issue that is important to be considered by various sector, especially in the business sector. In this case, both large and small companies (SMEs) need to think about the right strategy and technology to be applied in their business processes. The manufacturing industry has now evolved with 3D printing technology that makes it easy for business people to make prototypes quickly and cheaply. This study tries to look at the University as an institution that teaches entrepreneurship and incubates business through a Business Incubator. The focus is on what opportunities and strategies exist if Business Incubator in university implement 3D printing as a form of support and education for SMEs to make products and businesses more sustainable. The research method used is SWOT and TOWS analysis. The object of the research is the Business Incubator from a private university in Bandung, Indonesia. The result of this research is the identification of opportunities and positive impacts of using 3D printing in the Business Incubator. In addition, there are strategies that need to be implemented to implement them, in order to accommodate strengths and minimize constraints. This research is useful for universities to make policies and beneficial for SMEs to utilize 3D printing technology in their business processes.