“Designer” scaffolds for tissue engineering and regeneration

Tsur-Gang, Orna; Cohen, Smadar

doi:10.1560/378j-xmb1-nakf-ykq1

Cited by 36 publications

(27 citation statements)

References 82 publications

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“…In addition, the alginate scaffold enables ECM plasticity by allowing the seeded stromal cells and other penetrating cells to deposit their own ECM [43]. Moreover, degradation of the ECM via enzymatic cleavage, known to occur when using an ECMbased matrix, such as collagen [44], has been shown to propagate early inflammatory signals [45], which are unwanted when intending to create an immunoregulatory environment. Finally, the alginate scaffold can be modified to bind antigens and/or chemokines, thus increasing their local concentration within the matrix [35,39,46].…”

Section: Discussionmentioning

confidence: 99%

Stromal cell-induced immune regulation in a transplantable lymphoid-like cell constructs

et al. 2010

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Section: Discussionmentioning

confidence: 99%

Stromal cell-induced immune regulation in a transplantable lymphoid-like cell constructs

et al. 2010

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“…[21][22][23][24][25][26][27][28][29][30][31][32][33][34] On the other hand, a thin release coating provides a limited reservoir, and release may be effective only over a short period of time. The kinetics of release depend markedly on effects such as cross-linking and swelling of the hydrogel.…”

Section: Discussionmentioning

confidence: 99%

“…There has been considerable recent interest in the use of alginate or chitosan hydrogel coatings, sometimes with dispersed calcium phosphate particles, for orthopaedic and periodontal implants as well as nerve regeneration. [21][22][23][24][25][26][27][28][29][30][31][32][33][34] The concurrent release of active biomacromolecules may provide additional means of directing biological responses to implants, but prior to the design of clinical investigations it is essential to acquire in vitro data, and understand the performance and limitations of the release system. Thus, we here characterize the release behavior and release rates of both macromolecules and inorganic ions from or through hydrogel coatings, after detailed chemical, structural, and morphological characterizations of the coatings and the embedded nanoparticles by a range of analytical techniques.…”

Section: Introductionmentioning

confidence: 99%

Concurrent elution of calcium phosphate and macromolecules from alginate/chitosan hydrogel coatings

et al. 2008

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The concurrent release of calcium phosphate and biomacromolecules may improve wound healing responses at the interface with ceramic materials of orthopaedic and dental implants. Hydrogel coatings consisting of a mixture of alginate and chitosan were doped and applied onto solid carriers with the aim of investigating their use as local delivery vehicles. Coatings containing both the model macromolecule FITC-dextran 70 kDa (FD 70) and dispersed calcium phosphate carbonate (CPC) nanoparticles were coated onto a solid, nonporous model substrate to study the concurrent release of FD 70 and calcium and phosphate ions from within the hydrogel. Hydrogel coatings containing only FD 70 were cast onto porous calcium phosphate coatings, similar to hydroxyapatite, to study the release of FD 70 from, and calcium and phosphate ions through, the hydrogel coating. Transmission electron microscopy showed good dispersion of the CPC nanoparticles, and scanning electron microscopy and atomic force microscopy showed that increased CPC loading resulted in an increase in surface roughness but to extents well below those affecting cell responses. The release of FD 70 from CPC-loaded coatings was similar to release from the hydrogel alone, although higher CPC loadings resulted in small changes. The release of FD 70 was better described by double or triple phase zero order release kinetics; this complex time dependence indicates that in addition to outdiffusion, other, time-dependent factors apply, such as swelling of the gel, as expected from the known effects of calcium ions on alginate. Calcium and phosphate ions were also released, with similar release kinetics, through the hydrogel layer from the underlying CaP layer. In either case, release decreased to negligible levels after 3 days, suggesting that the systems of this study are suitable for short-term concurrent release of water-soluble biomacromolecules and calcium and phosphate ions.

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“…The re-design was bioinspired by the ECM roles, among them are adherence, migration, mechano-signals, growth factor presentation and stem cell differentiation [58]. The cues from the ECM, a complex network of collagen fibers, multi-adhesive matrix proteins and proteoglycans, have significant impacts on development during embryonic, fetal and neonatal stages as well as in adult tissues.…”

Section: Concept Designmentioning

confidence: 99%