Designer self-assembling hydrogel scaffolds can impact skin cell proliferation and migration

Bradshaw, Michael; Ho, Diwei; Fear, Mark W.; Gelain, Fabrizio; Wood, Fiona; Iyer, K. Swaminathan

doi:10.1038/srep06903

Cited by 76 publications

(57 citation statements)

References 42 publications

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“…(a)]. The inclusion of proteins/peptides like KLD‐12 and Arg‐Gly‐Asp (RGD) peptides to the synthetic scaffolds improves the biocompatibility and infiltration of the cells by providing the necessary functional groups and surface hydrophilicity . The increase in the porosity helps the cells to penetrate through the scaffolds and efficiently utilize the nutrients for its growth.…”

Section: Discussionmentioning

confidence: 99%

Improved regeneration potential of fibroblasts using ascorbic acid‐blended nanofibrous scaffolds

Sridhar

Venugopal

Ramakrishna

2015

J Biomedical Materials Res

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Two-dimensional scaffolds, three-dimensional scaffolds, and dermal substitutes are extensively used for biomedical applications in skin tissue regeneration. Not much explored synthetic polymers, like poly(l-lactic acid)-co-poly-(ε-caprolactone) (PLACL), natural polymers, like silk fibroin (SF), and active inducing agents, such as ascorbic acid (AA) and tetracycline hydrochloride (TCH), represent a favorable matrix for fabricating dermal substitutes to engineer artificial skin for wound repair. The profligate nature of residing skin cells near the wound site is a paramount to survival and also regulating stem cells and other cellular networks and mechanical forces. PLACL/SF/TCH/AA nanofibrous scaffolds were fabricated by electrospinning and characterized for fiber morphology, membrane porosity, wettability, and significant subchains using Fourier transform infrared spectroscopy for culturing human-derived dermal fibroblasts. The PLACL, PLACL/SF, PLACL/SF/TCH, and PLACL/SF/TCH/AA scaffolds obtained diameters between 250 and 340 nm. The secretion of collagen by the laboratory-grown fibroblasts over the AA-blended scaffolds was found to be significantly higher compared with that of other scaffolds. The obtained results positively prove that introduction of naturally secreting compounds (AA) by the cells into the nanofibrous scaffolds will favor cell's microenvironment and eventually leads to complete tissue regeneration.

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Section: Discussionmentioning

confidence: 99%

Improved regeneration potential of fibroblasts using ascorbic acid‐blended nanofibrous scaffolds

Sridhar

Venugopal

Ramakrishna

2015

J Biomedical Materials Res

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“…Additionally, bilayer hydrogel scaffolds (either in combination with a fibrous mat or porous scaffold) have also been reported for effective drug delivery to enhance wound healing and to improve skin regeneration via seeding of stem cells derived from debrided human burn skin [157,158]. More recently, self-assembling peptide-based hydrogel scaffolds have been reported to accelerate burn wound healing and skin cells proliferation [159,160] with brighter prospects for dermal tissue regeneration. …”

Section: Scaffolding Approaches and Different Types Of Scaffolds Imentioning

confidence: 99%

Future Prospects for Scaffolding Methods and Biomaterials in Skin Tissue Engineering: A Review

Chaudhari

Vig

Baganizi

et al. 2016

IJMS

466

288

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Over centuries, the field of regenerative skin tissue engineering has had several advancements to facilitate faster wound healing and thereby restoration of skin. Skin tissue regeneration is mainly based on the use of suitable scaffold matrices. There are several scaffold types, such as porous, fibrous, microsphere, hydrogel, composite and acellular, etc., with discrete advantages and disadvantages. These scaffolds are either made up of highly biocompatible natural biomaterials, such as collagen, chitosan, etc., or synthetic materials, such as polycaprolactone (PCL), and poly-ethylene-glycol (PEG), etc. Composite scaffolds, which are a combination of natural or synthetic biomaterials, are highly biocompatible with improved tensile strength for effective skin tissue regeneration. Appropriate knowledge of the properties, advantages and disadvantages of various biomaterials and scaffolds will accelerate the production of suitable scaffolds for skin tissue regeneration applications. At the same time, emphasis on some of the leading challenges in the field of skin tissue engineering, such as cell interaction with scaffolds, faster cellular proliferation/differentiation, and vascularization of engineered tissues, is inevitable. In this review, we discuss various types of scaffolding approaches and biomaterials used in the field of skin tissue engineering and more importantly their future prospects in skin tissue regeneration efforts.

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“…Recent studies have reported that functionalized SAP enhances angiogenesis, liver regeneration, skin cell proliferation, and the neural stem cell phenotype. [16][17][18][19] In a previous study, we found that SAP enhanced islet viability and function in vitro, 20 and identified functionalized SAP as a potential scaffold for islet transplantation.…”

mentioning

confidence: 90%

Functionalized self-assembling peptide improves INS-1 β-cell function and proliferation via the integrin/FAK/ERK/cyclin pathway

Liu

Chen

et al. 2015

IJN

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Islet transplantation is considered to be a curative treatment for type 1 diabetes mellitus. However, disruption of the extracellular matrix (ECM) leads to β-cell destruction and graft dysfunction. In this study, we developed a functionalized self-assembling peptide, KLD-F, with ECM mimic motifs derived from fibronectin and collagen IV, and evaluated its effect on β-cell function and proliferation. Atomic force microscopy and rheological results showed that KLD-F could self-assemble into a nanofibrous scaffold and change into a hydrogel in physiological saline condition. In a three-dimensional cell culture model, KLD-F improved ECM remodeling and cell-cell adhesion of INS-1 β-cells by upregulation of E-cadherin, fibronectin, and collagen IV. KLD-F also enhanced glucose-stimulated insulin secretion and expression of β-cell function genes, including Glut2 , Ins1 , MafA , and Pdx-1 in INS-1 cells. Moreover, KLD-F promoted proliferation of INS-1 β-cells and upregulated Ki67 expression by mediating cell cycle progression. In addition, KLD-F improved β-cell function and proliferation via an integrin/focal adhesion kinase/extracellular signal-regulated kinase/cyclin D pathway. This study highlights the fact that the β-cell-ECM interaction reestablished with this functionalized self-assembling peptide is a promising method to improve the therapeutic efficacy of islet transplantation.

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Designer self-assembling hydrogel scaffolds can impact skin cell proliferation and migration

Cited by 76 publications

References 42 publications

Improved regeneration potential of fibroblasts using ascorbic acid‐blended nanofibrous scaffolds

Improved regeneration potential of fibroblasts using ascorbic acid‐blended nanofibrous scaffolds

Future Prospects for Scaffolding Methods and Biomaterials in Skin Tissue Engineering: A Review

Functionalized self-assembling peptide improves INS-1 β-cell function and proliferation via the integrin/FAK/ERK/cyclin pathway

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Designer self-assembling hydrogel scaffolds can impact skin cell proliferation and migration

Cited by 76 publications

References 42 publications

Improved regeneration potential of fibroblasts using ascorbic acid‐blended nanofibrous scaffolds

Improved regeneration potential of fibroblasts using ascorbic acid‐blended nanofibrous scaffolds

Future Prospects for Scaffolding Methods and Biomaterials in Skin Tissue Engineering: A Review

Functionalized self-assembling peptide improves INS-1 &beta;-cell function and proliferation via the integrin/FAK/ERK/cyclin pathway

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Functionalized self-assembling peptide improves INS-1 β-cell function and proliferation via the integrin/FAK/ERK/cyclin pathway