“…Sensitive bio-sensing technologies such as surface plasmon resonance (SPR), electrochemical biosensors and recently inverse opal photonic crystals (IOPCs) biosensors, have been employed as diagnostic devices for the detection of biomarkers [ [3] , [4] , [5] , [6] , [7] , [8] ]. Photonic crystals (PCs) are dielectric and nanostructured materials with optical sensing properties and wide range applications in the field of optoelectronics and photonics [ [9] , [10] , [11] ]. Presently, PCs biosensors have been used for the selectively and sensitively detection of various biological analytes, such as DNA, cells, pathogens, proteins, antibodies (Ab) allows enhancing sensing performance in terms of limit-of-detection (LOD) [ 12 , 13 ].…”