Desinfestação de sementes e multiplicação in vitro de guabijuzeiro a partir de segmentos apicais juvenis (Myrcianthes pungens O.Berg) D. Legrand

Souza, Luana dos Santos de; Fior, Claudimar Sidnei; Souza, Paulo Vítor Dutra de; Schwarz, Sérgio Francisco

doi:10.1590/s0100-29452011005000081

Cited by 23 publications

(17 citation statements)

References 7 publications

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“…Rodríguez (2013) obtained a 68.00% germination result for the Red Strawberry Guava (P. cattleianum) using an LPm medium (Von Arnold & Erikson, 1981). Also, Souza, Fior, Souza and Schwarz (2011) achieved an average of 70% in vitro germination when placing Guabijuzeiro (Myrcianthes pungens) seeds in a WPM medium.…”

Section: Discussionmentioning

confidence: 99%

Different Methods for Overcoming Integumental Dormancy during in vitro Germination of Red Araza Seeds

Freire¹,

Gardin²,

Baratto³

et al. 2016

JAS

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Red Araza, or Red Strawberry Guava (Psidium cattleianum Sabine) is a native Brazilian Atlantic Forest species of the Myrtaceae family, whose seeds exhibit integumental dormancy. Due to its importance to different industries worldwide, recent research efforts are seeking to expand this species' micropropagation processes using in vitro seedling germination, especially since in vitro micropropagation of adult plant material has, so far, been limited. This research effort evaluated different methods of overcoming integumental dormancy during in vitro germination of the Red Araza, so as to allow future micropropagation of the species. The seeds' emergence and vigor were evaluated based on mechanical and acid scarification, using different substrates and immersions in solutions with different levels of gibberellic acid (GA 3 ), and on the influence of the pre-immersion of seeds in water and sulfuric acid. The mechanical and acid scarification of the seeds, combined or separate, resulted in higher in vitro germination percentages and a higher germination rate index (GRI). Pre-immersion in distilled water (20 hours) also proved to be efficient for the germination of the Red Araza seed, with 76.2% of the seeds germinating and a higher speed of emergence (GRI = 0.18). When compared to a Murashige and Skoog (MS-zero) medium, sowing in a hydrophilic cotton substrate showed greater emergence and vigor, with approximately 70% of the seeds germinating. Treating the seeds by pre-immersing them in GA 3 turned out to be unnecessary. The methods used for overcoming integumental dormancy during in vitro germination of Red Araza seeds proved to be efficient, and could be used to develop micropropagation protocols of seminal origin for this species.

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Section: Discussionmentioning

confidence: 99%

Different Methods for Overcoming Integumental Dormancy during in vitro Germination of Red Araza Seeds

Freire¹,

Gardin²,

Baratto³

et al. 2016

JAS

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“…In general, the best embryo development was obtained in the treatments with sodium hypocloride (T2 and T6). The sodium hypochlorite was also efficient in disinfestation and in vitro germination of other species such as guabiju [25] and jabuticaba [26]. Sodium hypochlorite, because of its oxidant potential, can increase the supply of oxygen for the seed, this would have influenced the increase of germination percentage in canjarana seeds, when sodium hypochlorite in different concentrations was tested [27].…”

Section: Resultsmentioning

confidence: 99%

Germination and Development of 'Precocinho' Peach Embryos: Asepsis and Use of PPMTM in Culture Medium

Nascimento¹,

Dini²,

Carpenedo³

et al. 2018

JEAI

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Embryo culture is a technique used to rescue immature embryos which would not germinate under natural conditions. However, one of the biggest problems of this process is the contamination by microorganisms. The objective of this work was to test asepsis methods and the use of PPM TM (Plant Preservative Mixture TM ) in the culture medium for the germination and development of 'Precocinho' peach embryos. After extraction from the fruits, seeds were submitted to treatments with thimerosal, sodium hypochlorite (NaClO), flame sterilization and moxifloxacin hydrochloride, in culture medium with and without addition of PPM TM , totaling nine treatments. The experimental design was completely randomized, with 20 replicates per treatment. Three evaluations were performed: in vitro establishment (at 55 days of cultivation), embryo development (at 65 days of cultivation) and acclimatization (20 days after transplanting in the greenhouse). Six treatments did Original Research ArticleTM is shown as the most efficient treatment, among the tested ones, for the germination and development of 'Precocinho' peach embryos.

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“…Novos métodos de desinfestação em sementes de espécies florestais são importantes, visando melhores desempenhos na germinação e diminuição na contaminação. Souza et al (2011) testaram diferentes concentrações de NaClO na desinfestação e germinação in vitro de sementes de guabijuzeiro (Myrcianthes pungens (Berg) Legr.). Os autores iniciaram com imersão das sementes em etanol 70% por 1 min, seguido de solução de NaClO nas concentrações de 0; 2; 4; 6 e 8% e observaram que a solução de NaClO de 4 a 6% de cloro ativo foi eficiente na melhoria da qualidade sanitária e não prejudicou a germinação.…”

Section: Resultsunclassified

Efeito da assepsia superficial na germinação e incidência de fungos em sementes de espécies florestais

et al. 2016

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O objetivo deste estudo foi testar tratamentos de assepsia e sua influência na germinação e sanidade de sementes de quatro espécies florestais: Bauhinia forficata, Cedrela fissilis, Parapiptadenia rigida e Senegalia bonariensis. As sementes foram submetidas aos seguintes tratamentos: controle (T0); NaClO 1% por 3 min (T1); NaClO 2% por 1 min + 1 enxágue com água destilada (T2); álcool 70% por 1 min + triplo enxágue em água destilada + NaClO 0,5% por 1 min + triplo enxágue com água destilada (T3); Lysoform® por 1 min (T4); e álcool 70% por 1 min + NaClO 1% por 1 min + 1 enxágue com água destilada por 1 min (T5). Após, as sementes foram submetidas ao teste de germinação, sendo registrado comprimento, massa fresca e massa seca de plântulas, e teste de sanidade. Para B. forficata recomenda-se o tratamento T2, com aumento do potencial germinativo e comprimento de plântulas, sendo o mesmo recomendado para C. fissilis para aumento da germinação e redução da incidência de Fusarium sp.; todos os tratamentos de assepsia reduzem a incidência de fungos de armazenamento em P. rigida, porém não há influência sobre a germinação; e para S. bonariensis não há necessidade de assepsia. Os tratamentos de assepsia recomendados podem ser utilizados rotineiramente em sementes das espécies florestais estudadas.

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Desinfestação de sementes e multiplicação in vitro de guabijuzeiro a partir de segmentos apicais juvenis (Myrcianthes pungens O.Berg) D. Legrand

Cited by 23 publications

References 7 publications

Different Methods for Overcoming Integumental Dormancy during in vitro Germination of Red Araza Seeds

Different Methods for Overcoming Integumental Dormancy during in vitro Germination of Red Araza Seeds

Germination and Development of 'Precocinho' Peach Embryos: Asepsis and Use of PPMTM in Culture Medium

Efeito da assepsia superficial na germinação e incidência de fungos em sementes de espécies florestais

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