RESUMO -O guabijuzeiro ocorre no Brasil desde São Paulo até o Rio Grande do Sul. Suas sementes são de curta longevidade e intolerantes à dessecação. O objetivo deste trabalho foi avaliar a qualidade fisiológica de sementes de guabijuzeiro ao longo de oito meses de armazenamento em câmara fria (5±1 ºC e 80% UR). Sementes de seis diferentes plantas localizadas os Municípios de Encruzilhada do Sul e Cachoeira do Sul, no Rio Grande do Sul, constituíram seis lotes, que foram avaliados quanto ao teor de água, à massa de mil sementes, à germinação, ao tempo médio de germinação em laboratório, à emergência e ao tempo médio de emergência em casa de vegetação. Até o terceiro mês de armazenamento, a viabilidade das sementes foi superior a 50%, porém três lotes apresentaram valores acima de 75%. Ao longo do período de armazenamento ocorreu pequena variação no teor de água (entre 40 e 45%), e as sementes de todos os lotes perderam qualidade. Contudo, aos oito meses foram registradas viabilidades entre 56% e 61% em sementes de dois lotes, mostrando que, em ambiente de câmara fria, é possível prolongar a longevidade de sementes de guabijuzeiro.Palavras-chave: Myrcianthes pungens, Sementes e Germinação. PHYSIOLOGICAL QUALITY OF GUABIJUZEIRO (Myrcianthes pungens (Berg) Legrand -MYRTACEAE) SEEDS UNDER STORAGE ABSTRACT -Guabijuzeiro is a woody plant and occurs in

RESUMO - DISINFESTATION OF SEEDS AND IN VITRO MULTIPLICATION OF GUABIJUZEIRO FROM APICAL JUVENILES SEGMENTS (Myrcianthes pungens O.BERG) D. LEGRAND ABSTRACT -The guabijuzeiro is an evergreen tree 15-25 meters high. Occurs in Brazil from São Paulo to Rio Grande do Sul. Among the wild fruits this species has several characteristics which make it with potential for commercial use, in which the most important are related to fructification. Its propagation is carried out by seed and little information on vegetative propagation. Hence, the objective was to study the disinfection of seeds and in vitro multiplication of guabijuzeiro. The experiments were performed at the Laboratory of Biotechnology in Horticulture, Faculdade de Agronomia, Universidade Federal do Rio Grande do Sul. The test began with the disinfestation soaking in 70% ethanol for 1 minute, followed by a solution of sodium hypochlorite at concentrations of 0; 2; 4; 6 and 8%. The culture medium used in experiments was WPM with 30 g.L -1 sucrose and 7 g.L -1 of agar. For the multiplication test were used apical segments of seedlings from seeds germinated in vitro. The treatments consisted of BAP concentrations (0; 0,2; 0,4; 0,6; 0,8; 1 and 2 mg.L -1 ). As a result the concentrations between 4 and 6% of sodium hypochlorite are more advantageous, as well as being effective in disinfestation, positively influence the germination. For multiplication, the cultivation of apical segments on medium with BAP at concentrations of up 1mg.l -1 was effective.

Chlamydomonas acidophila LAFIC-004 is an acidophilic strain of green microalgae isolated from coal mining drainage. In the present work, this strain was cultivated in acidic medium (pH 3.6) under phototrophic, mixotrophic, and heterotrophic regimes to determine the best condition for growth and lipid production, simultaneously assessing possible morphological and ultrastructural alterations in the cells. For heterotrophic and mixotrophic treatments, two organic carbon sources were tested: 1 % glucose and 1 % sodium acetate. Lipid content and fatty acid profiles were only determined in phototrophic condition. The higher growth rates were achieved in phototrophic conditions, varying from 0.18 to 0.82 day. Glucose did not result in significant growth increase in either mixotrophic or heterotrophic conditions, and acetate proved to be toxic to the strain in both conditions. Oil content under phototrophic condition was 15.9 % at exponential growth phase and increased to 54.63 % at stationary phase. Based on cell morphology (flow cytometry and light microscopy) and ultrastructure (transmission electron microscopy), similar characteristics were observed between phototrophic and mixotrophic conditions with glucose evidencing many lipid bodies, starch granules, and intense fluorescence. Under the tested conditions, mixotrophic and heterotrophic modes did not result in increased neutral lipid fluorescence. It can be concluded that the strain is a promising lipid producer when grown until stationary phase in acidic medium and under a phototrophic regime, presenting a fatty acid profile suitable for biodiesel production. The ability to grow this strain in acidic mining residues suggests a potential for bioremediation with production of useful biomass.

RESUMO -Os frutos do guabijuzeiro Myrcianthes pungens (Berg) Legrand têm alto potencial para exploração comercial. Sua propagação por sementes apresenta desvantagens como a segregação genética e prolongado período juvenil. A propagação por estaquia é uma alternativa para contornar estes problemas por ser econômica, rápida e simples. O objetivo do estudo foi avaliar o efeito de ácido 3-indolilbutírico (AIB) e do ácido ascórbico na propagação de guabijuzeiro por meio da estaquia em duas épocas do ano. Os tratamentos compreenderam estacas apicais com imersão em AIB nas concentrações de 2, 4 e 6 g.L -1 , ácido ascórbico (3 g.L -1 ). O delineamento foi completamente casualizado em arranjo fatorial. O enraizamento foi independente da utilização do AIB e ácido ascórbico, apresentando diferença na porcentagem de enraizamento em função da época de coleta com melhor resultado para estacas coletadas em fevereiro. É possível propagar Myrcianthes pungens a partir de estacas coletadas no verão, sem adição de AIB e ácido ascórbico. Palavras-chave: frutífera nativa, propagação vegetativa, recursos genéticosABSTRACT -Cloning of adult Myrcianthes pungens (Berg) Legrand specimen by cutting. The fruits of guabijuzeiro Myrcianthes pungens (Berg) Legrand have a high potential for commercial exploitation. Its propagation by seed has disadvantages, such as genetic segregation and prolonged juvenile period. The propagation by cuttings is an alternative to overcome these problems for being economical, fast and simple. The aim of the study was to evaluate the eff ect of 3-indolebutyric acid (IBA) and ascorbic acid in the propagation of guabijuzeiro by using the cutting method in two seasons. The treatments comprised apical cuttings with immersion in IBA with concentrations of 2, 4 and 6 g.L -1 , ascorbic acid (3 g.L -1 ). The result was completely randomized in a factorial arrangement. The rooting was independent of the use of IBA and ascorbic acid, presenting diff erence in rooting percentage depending on the time of collection with better result to cuttings collected in February. It is possible to propagate Myrcianthes pungens from cuttings collected in the summer without adding IBA and ascorbic acid.

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