Desmodium mottle virus, the first legumovirus (genus Begomovirus) from East Africa

Mollel, Happyness G.; Sseruwagi, Peter; Ndunguru, Joseph; Alicai, Titus; Colvin, John; Navas‐Castillo, Jesús; Fiallo‐Olivé, Elvira

doi:10.1007/s00705-017-3289-1

Cited by 9 publications

(6 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The deduced proteins shared highest similarities (E-values of 2.4e-5 to 2e-4 in BlastP searches; 19–24% depending on alignment algorithms; see also Fig. 1b ) with the capsid proteins (CP) of the grapevine-infecting grabloviruses 20 , 21 as well as desmodium mottle virus (DesMoV) 22 . The deduced size (24 kDa) of the two respective CFDV capsid proteins is in good agreement with the relative molecular mass estimated by SDS-polyacrylamide gel electrophoresis (PAGE) for the protein obtained from purified CFDV particles (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Remarkably, DNA-S.2, the gene of a variant virion, was maintained in the virus population from 1989 through 2015. CFDV CP has about equal sequence similarity (19–24%) to that of DesMoV, an Old World begomovirus infecting legumes 22 , and that of the grapevine-infecting grabloviruses 20 , 21 , which characterizes it as a sort of intermediate type of CP with elements of aleyrod- and cicadellid-transmitted viruses. It is interesting to note that CFDV is transmitted by the planthopper M. taffini 1 , 37 and grabloviruses by treehoppers (e.g.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Analysis of DNAs associated with coconut foliar decay disease implicates a unique single-stranded DNA virus representing a new taxon

Gronenborn¹,

Randles²,

Knierim³

et al. 2018

Sci Rep

View full text Add to dashboard Cite

The unique ecology, pathology and undefined taxonomy of coconut foliar decay virus (CFDV), found associated with coconut foliar decay disease (CFD) in 1986, prompted analyses of old virus samples by modern methods. Rolling circle amplification and deep sequencing applied to nucleic acid extracts from virion preparations and CFD-affected palms identified twelve distinct circular DNAs, eleven of which had a size of about 1.3 kb and one of 641 nt. Mass spectrometry-based protein identification proved that a 24 kDa protein encoded by two 1.3 kb DNAs is the virus capsid protein with highest sequence similarity to that of grabloviruses (family Geminiviridae), even though CFDV particles are not geminate. The nine other 1.3 kb DNAs represent alphasatellites coding for replication initiator proteins that differ clearly from those encoded by nanovirid DNA-R. The 641 nt DNA-gamma is unique and may encode a movement protein. Three DNAs, alphasatellite CFDAR, capsid protein encoding CFDV DNA-S.1 and DNA-gamma share sequence motifs near their replication origins and were consistently present in all samples analysed. These DNAs appear to be integral components of a possibly tripartite CFDV genome, different from those of any Geminiviridae or Nanoviridae family member, implicating CFDV as representative of a new genus and family.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Analysis of DNAs associated with coconut foliar decay disease implicates a unique single-stranded DNA virus representing a new taxon

Gronenborn¹,

Randles²,

Knierim³

et al. 2018

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Of all the fourteen genera, Begomovirus is the largest member of the group, which has about 445 species. Several begomoviruses have been reported to infect soybean [ 4 – 8 ]. In this study we characterized a new geminivirus named SGVA from diseased soybean stay-green plants showing leaf curling symptoms in Zhengzhou, China.…”

Section: Discussionmentioning

confidence: 99%

“…More than 67 viruses have been reported to infect soybean crops worldwide [ 3 ]. Among them viruses in the family Geminiviridae have been found in soybeans such as common bean curly stunt virus (CBCSV) [ 4 ], soybean chlorotic spot virus (SoCSV) [ 5 ], mungbean yellow mosaic India virus (MYMIV) [ 6 ], african cassava mosaic virus (ACMV) [ 7 ], cowpea golden mosaic virus (CPGMV), dolichos yellow mosaic virus (DoYMV) and soybean mild mottle virus (SbMMoV) [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of a new geminivirus from soybean plants and determination of V2 as a pathogenicity factor and silencing suppressor

Zhang

Lü

et al. 2022

BMC Plant Biol

View full text Add to dashboard Cite

Background Soybean is one of the four major crops in China. The occurrence of viruses in soybean causes significant economic losses. Results In this study, the soybean leaves from stay-green plants showing crinkle were collected for metatranscriptomic sequencing. A novel geminivirus, tentatively named soybean geminivirus A (SGVA), was identified in soybean stay-green plants. Sequence analysis of the full-length SGVA genome revealed a genome of 2762 nucleotides that contain six open reading frames. Phylogenetic analyses revealed that SGVA was located adjacent to the clade of begomoviruses in both the full genome-based and C1-based phylogenetic tree, while in the CP-based phylogenetic tree, SGVA was located adjacent to the clade of becurtoviruses. SGVA was proposed as a new recombinant geminivirus. Agroinfectious clone of SGVA was constructed. Typical systemic symptoms of curly leaves were observed at 11 dpi in Nicotiana benthamiana plants and severe dwarfism was observed after 3 weeks post inoculation. Expression of the SGVA encoded V2 and C1 proteins through a potato virus X (PVX) vector caused severe symptoms in N. benthamiana. The V2 protein inhibited local RNA silencing in co-infiltration assays in GFP transgenic 16C N. benthamiana plants. Further study revealed mild symptoms in N. benthamiana plants inoculated with SGVA-ZZ V2-STOP and SGVA-ZZ V2-3738AA mutants. Both the relative viral DNA and CP protein accumulation levels significantly decreased when compared with SGVA-inoculated plants. Conclusions This work identified a new geminivirus in soybean stay-green plants and determined V2 as a pathogenicity factor and silencing suppressor.

show abstract

“…Begomoviruses that are infecting legumes in the Old World are unique as they group into a separate cluster different from those of Old World and New World begomoviruses and have been termed ‘Legumoviruses’ (Briddon et al, 2010; Ilyas et al, 2010). At present, however, 11 distinct species often referred to as Legume Yellow Viruses—LYVs of the genus Begomovirus are known to infect various leguminous hosts and are transmitted by whitefly ( Bemisia tabaci ) (Qazi et al, 2007; Ilyas et al, 2009; Mollel et al, 2017; Siddell et al, 2020), and of them, seven have been reported from India and three species Mungbean yellow mosaic virus (MYMV), Mungbean yellow mosaic India virus (MYMIV) and Horsegram yellow mosaic virus (HgYMV) are known to cause YMD in different Vigna species (Malathi & John, 2008). With the characterization of a bipartite Dolichos yellow mosaic virus (DoYMV; Akram et al, 2015), the genomes of all legumoviruses are bipartite, except Soybean mild mottle virus (SbMMV) for which DNA‐B is not known (Alabi et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Species diversity, phylogeny and evidence of recombination in begomoviruses associated with yellow mosaic disease of moth bean (Vigna aconitifolia) in South India

Akram

Kamaal

Pratap

et al. 2022

Journal of Phytopathology

View full text Add to dashboard Cite

Polymerase chain reaction assays of eight yellow mosaic disease (YMD) affected moth bean samples using specific primers indicated the presence of Mungbean yellow mosaic virus (MYMV), Mungbean yellow mosaic India virus (MYMIV) and Horsegram yellow mosaic virus (HgYMV) in two samples (Mo1 and Mo5) and two of these three viruses in remaining six samples. Sequence data analysis of Mo1 and Mo2 revealed the involvement of multiple DNA‐A and DNA‐B molecules in both samples. Further analysis of DNA‐A and DNA‐B molecules confirmed the presence of three viruses viz., MYMIV, MYMV and HgYMV, in Mo1 and of two MYMV and HgYMV in Mo2 samples. Analysis of common region (CR) sequences of components (DNA‐A and DNA‐B) of each virus indicated >85% nucleotide identity confirming them to be cognate and species integrity. The recombination analysis revealed the presence of a total of six putative recombination events in the viruses under study. In a phylogenetic analysis of DNA‐A sequences of the viruses under study with bipartite legume‐infecting begomoviruses and some selected old and new world begomoviruses, MYMIV, MYMV and HgYMV grouped with respective virus species. Similar grouping and clustering were found in the DNA‐B sequences also. The present study reports infection by HgYMV in moth bean and the association of three viruses (MYMIV, MYMV and HgYMV) with moth bean yellow mosaic disease for the first time. The presence of mixed infection of begomoviruses provides opportunities for the exchange of DNA between the species which may lead to the development of new strain/virus species.

show abstract

Desmodium mottle virus, the first legumovirus (genus Begomovirus) from East Africa

Cited by 9 publications

References 26 publications

Analysis of DNAs associated with coconut foliar decay disease implicates a unique single-stranded DNA virus representing a new taxon

Analysis of DNAs associated with coconut foliar decay disease implicates a unique single-stranded DNA virus representing a new taxon

Identification and characterization of a new geminivirus from soybean plants and determination of V2 as a pathogenicity factor and silencing suppressor

Species diversity, phylogeny and evidence of recombination in begomoviruses associated with yellow mosaic disease of moth bean (Vigna aconitifolia) in South India

Contact Info

Product

Resources

About