Desulfovibrio bastinii sp. nov. and Desulfovibrio gracilis sp. nov., moderately halophilic, sulfate-reducing bacteria isolated from deep subsurface oilfield water

Abstract

Two moderately halophilic, mesophilic, sulfate-reducing bacteria were isolated from production-water samples from Emeraude Oilfield, Congo. Motile, vibrioid cells of SRL4225T grew optimally at a concentration of 4 % NaCl, at pH 5·8–6·2, with a minimal pH for growth of 5·2, showing that it is a moderately acidophilic bacterium. Cells of SRL6146T were motile, curved or vibrioid, long and thin rods. Optimal growth was obtained at a concentration of 5–6 % NaCl, at pH 6·8–7·2. The nutritional requirements showed that many of the characteristics of these strains overlap with those of known Desulfovibrio species. On the basis of 16S rRNA gene sequence analysis and DNA–DNA hybridization studies, both strains are members of the genus Desulfovibrio. However, they are not closely related to any species of the genus that have validly published names. It is therefore proposed that the two strains are members of two novel species of the genus Desulfovibrio with the names Desulfovibrio bastinii sp. nov. (type strain SRL4225T=DSM 16055T=ATCC BAA-903T) and Desulfovibrio gracilis sp. nov. (type strain SRL6146T=DSM 16080T=ATCC BAA-904T).

Microbial diversity in long-term water-flooded oil reservoirs with different in situ temperatures in China

“…Desulfovibrio was reported to be a major mesophilic genus that is recovered frequently from oil fields232425. Although no clone affiliated to the genus was found in the injected water sample, the indigenous nature of the detected Desulfovibrio -related bacteria remained unidentified without further culture-dependent assessment.…”

Analysis of Diversity and Activity of Sulfate-Reducing Bacterial Communities in Sulfidogenic Bioreactors Using 16S rRNA and dsrB Genes as Molecular Markers

“…only, since members of genus Desulfovibrio cannot use propionate as an electron donor. In reactor F, the sequence of 16S18 also clustered with members of the genus Desulfovibrio, with Desulfovibrio gracilis (27) as the closest relative. Desulfovibrio gracilis has been described as moderately halophilic SRB, with optimal NaCl concentrations for growth being 50 to 60 g liter Ϫ1 .…”

A Post-Genomic View of the Ecophysiology, Catabolism and Biotechnological Relevance of Sulphate-Reducing Prokaryotes

“…Other extremely halophilic SRB phylotypes affiliating with Desulfohalobiaceae and Desulfocella have also been detected in salterns (López-López et al, 2010), industrial wastewater ponds (Ben-Dov, Kushmaro, & Brenner, 2009) and notably in the Mediterranean deep-sea hypersaline (6 M NaCl) Lake Thetis (La Cono et al, 2011) as well as the Lake Kyros seawater-brine interphase containing up to 3 M MgCl 2 (Yakimov et al, 2014). Moderately halophilic SRB have repeatedly been reported, such as Desulfohalobium utahense isolated from sediments of the Great Salt Lake ( Jakobsen, Kjeldsen, & Ingvorsen, 2006) and Desulfovibrio bastinii originating from deep subsurface oilfield water (Magot, Basso, Tardy-Jacquenod, & Caumette, 2004). The physiological and molecular mechanisms of adaptation to high salinity, i.e., homeostasis, DNA and protein structures (Graziano & Merlino, 2014;Paul, Bag, Das, Harvill, & Dutta, 2008), have thus far not been studied in detail with SRB.…”