Detecção de lesões neoplásicas induzidas em mucosa oral de hamster utilizando espectroscopia de fluorescência

Silveira, Landulfo; Paleckis, Laura Guimarães Pagliuso; Nicolau, Renata Amadei; Nogueira, Grazielle Vilela; Busanello, Rosane Zélia; Mardegan, Daniela Aparecida; Fonseca, Sonia Maria; Zângaro, Renato Amaro; Pacheco, Marcos Tadeu Tavares

doi:10.1590/s0104-42302004000300038

Rev. Assoc. Med. Bras.

2004

DOI: 10.1590/s0104-42302004000300038

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Detecção de lesões neoplásicas induzidas em mucosa oral de hamster utilizando espectroscopia de fluorescência

Landulfo Silveira¹,

Laura Guimarães Pagliuso Paleckis²,

Renata Amadei Nicolau³

et al.

Abstract: RESUMO -OBJETIVO. Este trabalho teve por objetivo a utilização da técnica de espectroscopia de fluorescência induzida por laser para a caracterização de tecido normal e neoplásico em mucosa jugal de hamster, visando diagnosticar tecidos neoplásicos in vivo.MÉTODOS. O carcinógeno DMBA foi aplicado na bochecha direita de 31 hamsters com 150 ± 10g, três vezes por semana durante 12 semanas. Um animal foi mantido como controle (sem aplicação da droga). Após este período, os animais foram submetidos à espectroscopia… Show more

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Cited by 4 publications

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Spectroscopic characterization of oral epithelial dysplasia and squamous cell carcinoma using multiphoton autofluorescence micro‐spectroscopy

Pal

Edward

et al. 2017

Lasers Surg Med

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Objective Multiphoton autofluorescence microscopy (MPAM) has shown potential in identifying features that are directly related to tissue microstructural and biochemical changes throughout epithelial neoplasia. In this study, we evaluate the autofluorescence spectral characteristics of neoplastic epithelium in dysplasia and oral squamous cell carcinoma (OSCC) using multiphoton autofluorescence spectroscopy (MPAS) in an in vivo hamster model of oral neoplasia in order to identify unique signatures that could be used to delineate normal oral mucosa from neoplasia. Materials/Methods A 9,10-dimethyl-1,2-benzanthracene (DMBA) hamster model of oral precancer and OSCC was used for in vivo MPAM and MPAS. Multiphoton Imaging and spectroscopy were performed with 780 nm excitation while a bandpass emission 450–650 nm was used for MPAM. Autofluorescence spectra was collected through spectral window of 400–650 nm. Results MPAS with fluorescence excitation at 780 nm revealed an overall red shift of a primary blue-green peak (480–520 nm) that is attributed to NADH and FAD. In the case of oral squamous cell carcinoma (OSCC) and some high-grade dysplasia an additional prominent peak at 635 nm, attributed to PpIX was observed. The fluorescence intensity at 635 nm and an intensity ratio of the primary blue-green peak vs 635 nm peak, showed statistically significant difference between control and neoplastic tissue. Discussion Neoplastic transformation in the epithelium is known to alter the intracellular homeostasis of important tissue metabolites such as NADH, FAD and PpIX, which was observed by MPAS in their native environment. A combination of deep tissue microscopy owing to higher penetration depth of multiphoton excitation and depth resolved spectroscopy could prove to be invaluable in identification of cytologic as well as bio-molecular spectral characteristic of oral epithelial neoplasia.

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Spectroscopic characterization of oral epithelial dysplasia and squamous cell carcinoma using multiphoton autofluorescence micro‐spectroscopy

Pal

Edward

et al. 2017

Lasers Surg Med

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Laser-Induced Fluorescence at 488 nm Excitation for Detecting Benign and Malignant Lesions in Stomach Mucosa

et al. 2007

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This work aims the detection of the histopathologic alterations of in vitro human gastric mucosa using spectral informations from laser-induced fluorescence spectroscopy (LIFS) technique with excitation at 488 nm (argon laser). A total of 108 biopsies with endoscopic diagnosis of gastritis and gastric cancer were obtained at the antral gastric region, from 35 patients with dyspeptic digestive complaints. The biopsies were collected during the endoscopic examination. On each biopsy fragment the autofluorescence spectrum was collected in two random points, through a fiber-optic catheter coupled to the excitation laser. The fluorescence emission spectra collected by the fibers were directed to the spectrograph and detected by the CCD camera. The spectra were then separated in groups (N, normal; LI, light inflammation; MI, moderated inflammation; CA, adenocarcinoma), based on the histopathology. The ratio between the emission wavelengths 550 and 600 nm was used as a diagnostic parameter. Analysis of fluorescence spectra was able to identify the normal tissue from adenocarcinoma lesions with 100% of sensibility and specificity. The ratio intensities between inflammation (light and moderated), although presented significantly statistical differences when compared to the normal mucosa, do not furnish enough sensibility and specificity for use as an identification method due to high variations. LIFS, with excitation of 488 nm, could be used in the differentiation of normal tissue and neoplasic lesions, assisting a less invasive diagnosis.

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Fluorescence visualization efficacy for detecting oral lesions more prone to be dysplastic and potentially malignant disorders: a pilot study

Simonato

Tomo

Miyahara

et al. 2017

Photodiagnosis and Photodynamic Therapy

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Detecção de lesões neoplásicas induzidas em mucosa oral de hamster utilizando espectroscopia de fluorescência

Cited by 4 publications

References 11 publications

Spectroscopic characterization of oral epithelial dysplasia and squamous cell carcinoma using multiphoton autofluorescence micro‐spectroscopy

Spectroscopic characterization of oral epithelial dysplasia and squamous cell carcinoma using multiphoton autofluorescence micro‐spectroscopy

Laser-Induced Fluorescence at 488 nm Excitation for Detecting Benign and Malignant Lesions in Stomach Mucosa

Fluorescence visualization efficacy for detecting oral lesions more prone to be dysplastic and potentially malignant disorders: a pilot study

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