Detecção do gene mecA em estafilococos coagulase negativa resistentes à oxacilina isolados da saliva de profissionais da enfermagem

Rosa, Juliana de Oliveira; Moura, Josely Pinto de; Palos, Marinésia Aparecida Prado; Gir, Elucir; Reis, Cleômenes; Kipnis, André; Canini, Sílvia Rita Marin da Silva; Bellissimo-Rodrigues, Fernando; Pimenta, Fabiana Cristina

doi:10.1590/s0037-86822009000400008

Cited by 13 publications

(20 citation statements)

References 30 publications

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“…As previously mentioned, excessive consumption of different antibiotics has led to the emergence of multi-drug resistance in the developing countries (14). Aminoglycosides are an important group of antibiotics in the treatment of serious bacterial infections, especially Gram negative bacteria, but the current reports The methicillin resistance rate of 86% (89/103) among the study CNS isolates was higher than that of reported in Colombia (73%) (16), Egypt (75%) (17), and Brazil (77%) (18), which could be due to the overuse of antibiotics that eradicate MSCNS and facilitate MRCNS colonization. Furthermore, activity in screening, outbreak investigations, and contact tracing regarding methicillin-resistant coagulasenegative Staphylococci (MRCoNS) may differ between Iran and the abovementioned countries.…”

Section: Discussionmentioning

confidence: 78%

Distribution of Aminoglycoside Resistance Genes in Coagulase-Negative Staphylococci Isolated From Hospitalized Patients

Sabzehali

Goudarzi

et al. 2017

Arch Pediatr Infect Dis

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Background: The incidence of coagulase-negative staphylococci (CNS) isolates, as causes of hospital-acquired infections, is increasing annually and it is a truly global challenge. Objectives: The current study aimed at assessing aminoglycoside resistance genes among CNS isolated from hospitalized patients and investigating the prevalence of methicillin and aminoglycoside resistance genes using molecular methods. Methods: A total of 103 species of coagulase negative staphylococci were isolated from clinical specimens from August 2013 to November 2014. All the specimens were identified using conventional microbiological methods including colony morphology, Gram staining, catalase, and slide and tube coagulase. The isolates were subjected to the API-20 Staph identification kit. Antibiotic susceptibility tests were performed using Kirby-Bauer disc diffusion method. The methicillin and aminoglycoside resistant genes among coagulase negative staphylococci were detected by polymerase chain reaction (PCR) and sequencing methods. Results: The most frequent clinically isolated coagulase negative staphylococci species were Staphylococcus hominis and S. haemolyticus (51.9%), S. epidermidis (21.7%), S. lugdunensis (9.8%), S. intermedius (4.9%), S. saprophyticus and S. simulans (3.9%), S. warneri (2.9%), as well as S. chromogenes, S. sciuri, S. caprae, S. schleiferi, and S. xylosus (0.98%). The overall rate of methicillin resistance among CNS species in the present study was 89 (86.4%). The resistance of CNS isolates against tested antibiotics was as follows: 74 (71.8%) to cefoxitin, 54 (52.4%) to kanamycin, 51 (49.5%) to gentamicin, 45 (43.7%) to tobramycin, and 16 (15.5%) to amikacin. The prevalence of aminoglycoside resistance genes such as ant (4')-Ia, aac (6')/aph (2") and aph (3')-IIIa were 89 (86.4%), 87(84.5%), and 68(66%), respectively. Conclusions: The presence of antibiotic resistance among coagulase negative Staphylococcus species, which cause nosocomial infections, has increased. Therefore, identification of coagulase negative staphylococci within 24 hours after development of enzymatic tests would be very useful in any clinical microbiology laboratory for effective treatment. Resistance to antibiotics, including aminoglycosides, develops quickly in CNS species where these antimicrobial agents are widely used. Thus, a better understanding of antibiotic resistance of CNS species is essential to eliminate antibiotic resistance in hospitals and society.

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Section: Discussionmentioning

confidence: 78%

Distribution of Aminoglycoside Resistance Genes in Coagulase-Negative Staphylococci Isolated From Hospitalized Patients

Sabzehali

Goudarzi

et al. 2017

Arch Pediatr Infect Dis

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“…En el presente estudio fueron identificadas cepas de Staphylococcus coagulasa negativos resistentes a la meticilina (ECN). Dichos microorganismos son de extrema importancia, pues colonizan naturalmente la piel y las mucosas, causando infecciones en el sitio primario de infección o pueden incluso diseminarse, causando bacteriemia y septicemia 16 . Los ECN son frecuentemente reconocidos como una de las principales causas de infecciones de la corriente sanguínea intrahospitalarias en pacientes críticos, pudiendo llevarlos a la morbilidad e incluso al deceso 17 .…”

Section: Discussionunclassified

“…Los estudios refieren que la prevalencia de ECN resistentes a oxacilina es elevada en la mayoría de los hospitales de Brasil y del mundo, y la identificación de las especies de dicho grupo es de extrema importancia, ya que se las relaciona frecuentemente con septicemias neonatales, infecciones en pacientes inmuno-comprometidos y bajo nutrición parenteral total, además de pacientes postquirúrgicos y usuarios de dispositivos intravasculares 16,18 .…”

Section: Discussionunclassified

Trabajadores del área de salud de un hospital oncológico colonizados por microorganismos multidroga resistentes

Trindade

Rodrigues

Sousa

et al. 2014

eglobal

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“…Percentuais de resistência menores foram obtidos por alguns autores. ROSA et al (2009), em um estudo em que se pesquisava o ECN na saliva de profissionais de enfermagem encontraram 32% de resistência à oxacilina. Porcentagens ainda menores foram encontradas por PEREIRA & CUNHA (2009), onde 16,2% dos ECN isolados de amostras nasais de estudantes de enfermagem foram resistentes à oxacilina.…”

Section: Identificação Dos Ecn Resistência Antimicrobianaunclassified

“…Entretanto, o uso indiscriminado de antibióticos promove o aparecimento de estirpes multirresistentes tornando necessário utilizar antibióticos de maior espectro para o tratamento de infecções causadas por estes microrganismos (OLIVEIRA & LIMA, 2010). ROSA et al (2009) encontraram resistência elevada de ECN à mupirocina. Este antimicrobiano é utilizado para descolonização de indivíduos portadores de Staphylococcus aureus resistente a oxacilina.…”

Section: Identificação Dos Ecn Resistência Antimicrobianaunclassified

Colonização Nasal Por Estafilococos Coagulase Negativa Em Profissionais De Uma Instituição De Saúde Na Região Norte Do Espírito Santo

Santos¹,

Matos²,

Gradella³

2015

Enciclopédia Biosfera

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The bacteria of the genus Staphylococcus present as Gram-positive cocci, grouped in clusters and are mainly found in normal human flora (skin and mucosa). They are classified by the ability to clot plasma coagulase positive or negative coagulase. The coagulase-negative Staphylococci (CNS) have become relevant in the clinic after the identification of these microorganisms in infections. The emergence of antimicrobialresistant strains in hospitals raises concerns regarding these microorganisms. This study aimed to evaluate the frequency of nasal colonization by CNS and its resistance profile methicillin professionals of a health institution in the region northern of the Espirito Santo. Samples were collected from the nasopharynx of volunteers, isolated Mannitol Salt Agar and identified by Gram staining technique, the catalase test and free coagulase. The methicillin resistance profile was evaluated by susceptibility of the organism against the oxacillin disk. Of the 38 samples collected, isolated in 31 (81.6%) the CNS. Of 30 samples CNS assayed for sensitivity to oxacillin, 23 (76.7%) showed resistance. It was found that professionals in direct contact with patients have more frequently resistant CNS. Therefore, it is considered important to the care of the spread of resistant strains of microorganisms by professionals in a hospital setting, avoiding causing major damage to the health of patients and higher expenses for health services.

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Detecção do gene mecA em estafilococos coagulase negativa resistentes à oxacilina isolados da saliva de profissionais da enfermagem

Cited by 13 publications

References 30 publications

Distribution of Aminoglycoside Resistance Genes in Coagulase-Negative Staphylococci Isolated From Hospitalized Patients

Distribution of Aminoglycoside Resistance Genes in Coagulase-Negative Staphylococci Isolated From Hospitalized Patients

Trabajadores del área de salud de un hospital oncológico colonizados por microorganismos multidroga resistentes

Colonização Nasal Por Estafilococos Coagulase Negativa Em Profissionais De Uma Instituição De Saúde Na Região Norte Do Espírito Santo

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