Detecção do virus ‘HoBi’-like (BVDV-3) em bovino no semiárido do Estado da Paraíba

Abstract

RESUMO.-Objetivou-se descrever os aspectos clínicos e anatomopatológicos, e a identificação viral de um caso de infecção pelo vírus 'Hobi'-like (BVDV-3) em bovino do semiárido paraibano, Nordeste do Brasil. Um bovino, fêmea, três meses de idade, foi levado ao Hospital Veterinário da UFCG apresentando salivação, dificuldade de apreensão do teto, falta de apetite, fezes escuras e em pouca quantidade. Diante da piora do quadro clínico optou-se por sua eutaná-sia in extremis, seguida da realização da necropsia e coleta de material para histopatologia. Histologicamente, nas mucosas do trato digestivo, havia edema, degeneração balonosa, necrose e infiltrado inflamatório, que foi observado na face dorsal da língua e no seu epitélio mais profundo. The aim of this study was to describe the clinical and anatomopathological aspects, and the viral identification of a case of 'Hobi'-like (BVDV-3) vírus infection in cattle from the semiarid of Paraiba State, Northeastern Brazil. A female bovine, three months old, was sent to the UFCG's Veterinary Hospital presenting salivation, difficulty of ceiling seizure, lack of appetite, dark feces and in small amounts. Due to worsening of symptoms it was decided to in extremis euthanasia, followed by the necropsy and collection of material for histopathology. Histologically, in the mucous membranes of the digestive tract there were edema, ballooning degeneration, necrosis and inflammatory infiltrate, which was observed on the dorsal surface of the tongue and in its deepest epithelium. The immunohistochemical of skin biopsies of the extremity of the ear (ear notches) showed positive antigenic marking and by RT-PCR it was possible to detect viral RNA of BVDV in the serum, whose cytopathic effect in epithelial cells of bovine kidney lineage "Madin Darby bovine kidney" (MDBK)was not observed. The sequencing of the 5'NCR gene showed that the virus isolated was more related to 'Hobi'-like (BVDV-3). After confirming the diagnosis serum samples were collected from 23 animals for serology by indirect ELISA, and a seropositivity of 69.6% (16/23) was found. The identification of this new case of 'Hobi'-like infection in Paraiba reaffirms the need for regular BVDV monitoring in the region to early detection of infection in the herds and adoption of effective preventive and control measures.INDEX TERMS: Cattle, BVDV-3, pestivirus, viral identification.

A genetic profile of bovine pestiviruses circulating in Brazil (1998–2018)

“…BVDV-1 and BVDV-2 have the broadest global distribution and have been associated with a variety of clinical manifestations in cattle (Houe, 2003) and a wide range of ruminant species, both domestic and free ranging. HoBiPeV were initially identified as contaminants of fetal bovine serum (FBS) of Brazilian origin (Schirrmeier et al ., 2004) and subsequently associated with a variety of clinical manifestations in cattle in South America (Cortez et al ., 2006; Decaro et al ., 2011; Bauermann et al ., 2013; Marques et al ., 2016; Weber et al ., 2016 a , 2016 b ; Mósena et al ., 2017 a , 2017 b ; Silveira et al ., 2017), Italy (Decaro et al ., 2011), Thailand (Liu et al ., 2009 a , 2009 b ), India (Mishra et al ., 2014), and Bangladesh (Haider et al ., 2014). It has also been isolated from water buffalo.…”

“…In the last two decades (1998–2018), selected BVDV-1 and BVDV-2 subgenotypes have been shown to circulate among Brazilian cattle and other related species (Canal et al ., 1998; Gil et al , 1998; Flores et al ., 2002; Cortez et al ., 2006; Lunardi et al ., 2008; Bianchi et al ., 2011, 2017; Otonel et al ., 2014; Weber et al ., 2014; Mósena et al ., 2017 a , 2017 b ; Silveira et al ., 2017; Monteiro et al ., 2018 a , 2018 b ). Likewise, atypical pestiviruses (subsequently identified as HoBiPeV) have been isolated from animals since the early 2000s and a number of studies indicate that these viruses are endemic in Brazil (Cortez et al ., 2006; Bianchi et al ., 2011; Marques et al ., 2016; Weber et al ., 2016 a , 2016 b ; Mósena et al ., 2017 a , 2017 b ; Silveira et al ., 2017, 2018; Monteiro et al ., 2018 a , 2018 b ). This article provides a critical review of the published work concerning the genetic identification and genotyping of bovine pestiviruses, with the goal of generating an approximate genetic profile of Brazilian bovine pestiviruses.…”

“…Thus, isolate D32/00_‘HoBi’ was proposed as the prototype of a novel pestivirus genetic group, variously referred to as HoBi -like , atypical pestiviruses or, simply, BVDV-3 (Liu et al ., 2009 a , 2009 b ; Decaro et al ., 2011; Bauermann et al ., 2013). Viruses belonging to this genetic group were subsequently found in FBS from different geographical origins and in clinical specimens obtained from cattle with a variety of clinical manifestations, in South America (Cortez et al ., 2006; Marques et al ., 2016; Weber et al ., 2016 a , 2016 b ; Mósena et al ., 2017 a ; Monteiro et al ., 2018 a ; Silveira et al ., 2018), Italy (Decaro et al ., 2011), Thailand (Liu et al ., 2009 a , 2009 b ), India (Mishra et al ., 2014), and Bangladesh (Haider et al ., 2014). Recently, this group of viruses has been recognized as a new pestivirus species, named Pestivirus H (ICTV, 2017) (proposed abbreviation, HoBiPeV).…”

“…A HoBiPeV strain – identified by analysis of the 5′UTR – was associated with a case of acute, fatal gastroenteric disease in the semi-arid region of Paraiba state (PB), northeastern Brazil (Marques et al ., 2016). A HoBiPeV was also identified in a spleen sample from a dead calf presenting signs of acute BVDV in a beef cattle herd in Mato Grosso (MT).…”

Sensitivity and specificity of indirect ELISA for the detection of antibody titers against BVDV from beef cattle raised in Pará State

“…The isolates of BVDV present a great antigenic variability, and two antigenically distinct genotypes have already been identified: BVDV-1 and BVDV-2 (VILCEK et al, 2001). There is a growing awareness of another atypical or HoBi-like pestivirus (BVDV-3), which is also associated with clinical disease in cattle (BAUERMANN et al, 2013;MARQUES et al, 2016).…”