Detección de Brucella abortus por PCR en sangre y leche de vacunos

Bernal, Vanesa; Muskus, L Carlos; Berdugo, G Jesús

doi:10.21897/rmvz.382

Cited by 5 publications

(6 citation statements)

References 9 publications

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“…Baek et al (2005) used spleen macerates to identify intrauterine infection by B. abortus in mice progeny because whole blood PCR results were variable (Baek et al, 2005); however, Cevallos et al (2008) have proposed to improve the brucellosis diagnostic procedure using whole blood assuming that primers election do not affect the test sensitivity, as suggested by Leal-Klevezas et al (1995, 2000 and Sreevatsan et al (2000) who have also used PCR with blood samples. In fact, Mosquera et al (2008) corroborated the usefulness of PCR testing for blood brucellosis diagnosis with better results than using milk samples. Biology molecular assays conducted to identify S19 vaccine strain using milk provided acceptable results.…”

Section: Discussionmentioning

confidence: 59%

Identification of immunotolerance in the progeny of cows infected with Brucella abortus

Martínez-Herrera

Padrón-Tello²,

Peniche-Cardentilde³

et al. 2012

Afr. J. Microbiol. Res.

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The progeny of cows infected with Brucella abortus could acquire the bacterium during their fetal stage and generate immune tolerance. In order to identify them, a clinical assay was conducted with two groups of seronegative calves. In Group I, seven bovine females received a standard S19 dose, and in Group II, eight males served as control. All animals were sampled seven times by official serological tests every 45 days. Also, complete blood with anticoagulant was collected and tested by the polymerase chain reaction (PCR) 45 and 135 days after vaccination. Results were analyzed by the test of proportions. In Group I, all the animals had their maximum antibodies title at the first sampling, but from the fourth sampling, their titles decreased until they became undetectable for the screening test. All animals in Group II remained negatives. Animals from both groups recorded negative with PCR. Significant differences (P<0.05) between groups were observed for seroconversion in the first two samplings. It was concluded that none of the tested animals were immunotolerant and that PCR may not be an appropriate method to demonstrate immunotolerence in some individuals.

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Section: Discussionmentioning

confidence: 59%

Identification of immunotolerance in the progeny of cows infected with Brucella abortus

Martínez-Herrera

Padrón-Tello²,

Peniche-Cardentilde³

et al. 2012

Afr. J. Microbiol. Res.

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“…en quesos distribuidos en Barranquilla. Este agente patógeno se ha encontrado previamente en las etapas de producción primaria de leche en otras ciudades de Colombia (20,21). En el estudio se reconfirmó, además, la presencia de Salmonella spp.…”

Section: Discussionunclassified

“…Aunque el método estándar o cultivo microbiológico tradicional tiene un gran valor en los estudios epidemiológicos, su sensibilidad es baja y la confirmación de los resultados tarda entre cuatro y cinco días (32), por lo tanto, es necesario emplear metodologías más sensibles de diagnóstico, como la qPCR (21). Aunque la técnica de qPCR detecta el ADN de los microorganismos y no determina su viabilidad, se pudo confirmar su aplicabilidad para detectar microorganismos de difícil aislamiento por su elevado límite de detección.…”

Section: Discussionunclassified

Detección molecular de Salmonella spp., Listeria spp. y Brucella spp. en queso artesanal fresco comercializado en Barranquilla: un estudio piloto

et al. 2018

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Introduction: Each year approximately 3 million people die as the result of foodborne diseases. The fresh artisan (handmade) cheese produced and distributed in the Colombian Caribbean region is a native product from the departments of Córdoba, Sucre, Bolívar, Atlántico, Magdalena, Cesar, and La Guajira. Its mass consumption increases the risk of infection with Salmonella spp., Listeria spp., and Brucella spp., as it is made with a very rustic technology, with unpasteurized cow milk, without standardized and hygienic procedures and its storage is inadequate. Objective: To detect the presence of Salmonella spp., Listeria spp., and Brucella spp. in samples of fresh artisan cheese from the Colombian Caribbean region. Materials and methods: Twenty-seven samples of cheese from five departments of the Caribbean Region (Atlántico (n=6), Bolívar (n=2), Córdoba (n=1), Magdalena (n=16), and Sucre (n=2)) were analyzed by real-time polymerase chain reaction (qPCR). Seventeen of the samples corresponded to soft cheese, five to semi-hard cheese and five to hard cheese. Results: In 62.9% (17/27) of the samples we detected Salmonella spp., in 70.4% (19/27), Listeria spp., and in 22.2% (6/27), Brucella spp., mainly from the department of Magdalena. In 62.5% (10/16) of the samples we detected Salmonella spp. and Listeria spp. while in the department of Atlántico, 50% (3/6) of the samples corresponded to Brucella spp. Conclusion: The results confirmed the presence of these microorganisms in all the samples of soft cheese from the Colombian Caribbean region.

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“…La identificación y tipificación de Brucella spp. se realiza mediante métodos inmunológicos y moleculares (62,119).…”

Section: 31unclassified

Identificación de Riesgos Biológicos Asociados al Consumo de Leche Cruda Bovina en Colombia

2012

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Detección de Brucella abortus por PCR en sangre y leche de vacunos

Cited by 5 publications

References 9 publications

Identification of immunotolerance in the progeny of cows infected with Brucella abortus

Identification of immunotolerance in the progeny of cows infected with Brucella abortus

Detección molecular de Salmonella spp., Listeria spp. y Brucella spp. en queso artesanal fresco comercializado en Barranquilla: un estudio piloto

Identificación de Riesgos Biológicos Asociados al Consumo de Leche Cruda Bovina en Colombia

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