Detección serológica y caracterización molecular de <i>Potato virus S</i> (PVS, <i>Carlavirus</i<) en cultivos de papa de Colombia

Abstract

Detection and molecular characterization of Potato virus S (PVS, Carlavirus) from Colombia. In Colombia, potato crops are affected by a wide variety of viruses such as PVY, PLRV, PVX, PMTV and PVS. Unfortunately, there are very few studies on the biology, distribution and pathogenicity of these viruses; this situation is even worse for the latent virus PVS. In this work, we evaluated the presence of PVS in four Colombian provinces (Antioquia, Boyacá, Cundinamarca, Nariño) by the use of ELISA. We also studied the degree of molecular variation by sequence comparison of a segment of the gene encoding for the viral coat protein. In average, PVS was detected in 40% of 320 analyzed samples of potato leaves; the highest levels were observed in the East of Antioquia (49%) and Pasto (Nariño) (47%), while in the other regions ranged between 35% and 42%. Analysis of sequence revealed the presence of two PVS strains in Colombia: three isolates were associated to PVS O (Ordinary) and twelve belonged to PVS A (Andean). A high diversity was observed among PVS A strains with percent identities in the range of 88-99%. These findings highlight the importance of strengthening seed certification programs and quarantine measures in Colombia for viruses like PVS, which can cause losses of up to 20% in potato crops and even higher in mixed virus infection.

Caracterización molecular del Potato virus V (PVV) infectando Solanum phureja mediante secuenciación de nueva generación

“…Además del PYVV, otros virus detectados con altos niveles de incidencia en S. phureja incluyen el Potato virus Y (PVY), Potato virus S (PVS), Potato virus X (PVX) y Potato leafroll virus (PLRV) (Gil et al, 2013;Gutiérrez et al, 2013;Hernández-Guzmán y Gúzman-Barney, 2014;Medina et al, 2015;García et al, 2016).…”

Molecular Analysis of the Global Population of Potato Virus S Redefines Its Phylogeny, and Has Crop Biosecurity Implications

“…Subsequently, Cox and Jones [18] suggested the terms PVS O-CS (CS = Chenopodium systemic) for major phylogroup PVS O isolates which invaded this host systemically, and PVS A-CL (CL = Chenopodium localized) for major phylogroup PVS A isolates that remained localized within inoculated leaves. Moreover, when several Andean PVS isolates from S. phureja in Colombia were sequenced, an additional major phylogroup was found [19][20][21]. This was named PVS RVC [5].…”

DETECCIÓN Y CARACTERIZACIÓN MOLECULAR DEL POTATO VIRUS B (PVB) EN CULTIVOS DE PAPA CRIOLLA (Solanum phureja) EN ANTIOQUIA

“…Lagerh) y diversas enfermedades de origen viral (Navas y Díaz, 2012). Los agentes causales de las enfermedades virales hasta ahora detectados en este cultivo en Antioquia incluyen Potato leafroll virus (PLRV), Potato mop-top virus (PMTV), Potato virus S (PVS), Potato virus V (PVV), Potato virus X (PVX), Potato virus Y (PVY) y Potato yellow vein virus (PYVV) (Sánchez et al, 1991;Gil et al, 2011Gil et al, , 2012Gil et al, , 2013Guzmán et al, 2012;Villamil-Garzón et al, 2014;Álvarez et al, 2016;Gutiérrez et al, 2016;Mesa et al, 2016;Vallejo et al, 2016;Gallo et al, 2019).…”