Detecting Clonal Rearrangement in Non-Hodgkin's Lymphomas in Taiwan by Polymerase Chain Reaction

Chuang, Shih Sung; Lin, Chih‐Ming; Shen, Fan; Liao, Pei Ju; Liao, Yung Liang; Chang, Jae Bong; Tsai, Yi Chang; Cho, Chi Yi; Huang, Wenya

doi:10.1080/1042819021000040314

Cited by 16 publications

(9 citation statements)

References 18 publications

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“…22 For FFPE samples, the sensitivity has been reported to range from 80 to 98% for TCR. [23][24][25][26] ENKTLs are frequently accompanied by necrosis and apoptosis of the tumor cells, leading to degradation of DNA, ineffective PCR reactions, and false-negative results. 27 In a study by Pongpruttipan et al, 8 clonal TCR gene rearrangements were documented in only three out of six TCR protein-positive cases.…”

Section: Discussionmentioning

confidence: 99%

Nasal-type NK/T-cell lymphomas are more frequently T rather than NK lineage based on T-cell receptor gene, RNA, and protein studies: lineage does not predict clinical behavior

et al. 2016

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Extranodal natural killer (NK)/T-cell lymphoma (ENKTL), nasal type, comprises NK or cytotoxic T cells. We evaluated the clinical impact of cell type and the usefulness of T-cell receptor (TCR) gene transcripts in distinguishing cell lineage. One hundred and eight cases of ENKTL were analyzed for TCR gene rearrangements using the BIOMED-2 protocol and for TCR gene expression using immunohistochemistry for TCR-βF1 and TCR-cγM1, and RNA in situ hybridization for TCR gene transcripts. Prognostic factors were analyzed. Among the 108 cases, 44 were monoclonal for a TCR rearrangement (40%) while 64 (60%) were undefinable. The monoclonal cases expressed TCR-βF1 in 14 out of 40 cases (35%) and TCR-cγM1 in 1 out of 44 cases (2%). The 64 undetermined cases expressed TCR-βF1 in 15 cases (23%) and TCR-cγM1 in 1 (2%). Thirteen of 40 TCR-β constant gene transcript-positive cases (33%) expressed TCR-βF1 and one of nine TCR-γ constant gene transcript-positive cases (11%) expressed TCR-cγM1. TCR gene transcripts were not useful in the distinction of cell lineages. TCR gene transcripts were positive in ENKTLs as well as in normal B cells and aggressive NK-cell leukemia. Based on gene rearrangements and immunohistochemistry for TCR, there were 60 T-cell type cases (56%), 32 NK-cell type cases (30%), and 16 cases with an undetermined cell type (14%). TCR protein was expressed in 30/60 T-ENKTLs (50%) in a variable fraction of tumor cells. There were no significant differences in clinical findings or overall patient survival between T-or NK-cell types of ENKTL, although those with a T-cell type tended to show a better prognosis for those with localized nasal lymphomas. Univariate and multivariate analysis showed that a non-nasal ENKTL, age 460 years, high level of lactate dehydrogenase, bone marrow involvement, and the absence of radiotherapy were independent prognostic factors.

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Section: Discussionmentioning

confidence: 99%

Nasal-type NK/T-cell lymphomas are more frequently T rather than NK lineage based on T-cell receptor gene, RNA, and protein studies: lineage does not predict clinical behavior

et al. 2016

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“…Genomic DNA was extracted from formalin-fixed, paraffin-embedded tissues by using a Qiagen DNeasy Blood & Tissue Kit (Qiagen; Hilton, Germany) according to the manufacturer's instructions. The primers for TCRγ chain gene and the protocol for PCR were designed as previously described [15]. A case of PTCL-NOS was used as positive control, and a normal lymph node as a negative control.…”

Section: Polymerase Chain Reaction Analysis For Tcr Chain Gene Rearramentioning

confidence: 99%

Transcription factors engaged in development of NK cells are commonly expressed in nasal NK/T-cell lymphomas

Zhang

et al. 2011

Human Pathology

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“…Although previously reported frequencies of B-cell clonality in AILT are similar, 20,23,32,33 those for PTCL range from 9 to 16%. 2,4,5,22,34,35 We considered that some cases of PTCL-NOS were misclassified and actually represent AILT. However, PTCL-NOS cases did not have classic morphologic features of AILT.…”

Section: Discussionmentioning

confidence: 99%

The Frequency of B- and T-Cell Gene Rearrangements and Epstein-Barr Virus in T-Cell Lymphomas

Tan

Warnke

Arber

2006

The Journal of Molecular Diagnostics

116

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We report on a series of 58 cases of angioimmunoblastic T-cell lymphoma (AILT) and 59 cases of peripheral Tcell lymphoma, unspecified (PTCL-NOS). Subsets of cases from both diagnostic groups were complicated by associated B-cell proliferations, and we performed Band T-cell clonality studies and in situ hybridization for Epstein-Barr virus (EBV) to investigate the relationship between B-cell proliferation, B-cell clonality, and EBV. Using multiplex polymerase chain reaction assays based on the BIOMED-2 collaborative study, we detected TCR␥ T-cell clones in 78 and 81% of AILT and PTCL-NOS cases, respectively, and IGH B-cell clones in 34 and 35% of AILT and PTCL-NOS cases, respectively. The majority of cases contained EBV-positive cells, including 50% of AILT and 57% of PTCL-NOS cases, and cases with B-cell proliferations were more often EBV-positive. Although a relatively high rate of B-cell clonality has been shown for AILT, our findings for PTCL-NOS differ from previous reports in that B-cell clonality was relatively frequent. Overall, a positive B-cell clone correlated, in part, with the presence of a B-cell proliferation but not with EBV. Our findings demonstrate that B-cell clonality is a common finding in AILT and PTCL-NOS, and its presence should not negate the diagnosis established by morphologic, immunophenotypic, and clinical findings.

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Detecting Clonal Rearrangement in Non-Hodgkin's Lymphomas in Taiwan by Polymerase Chain Reaction

Cited by 16 publications

References 18 publications

Nasal-type NK/T-cell lymphomas are more frequently T rather than NK lineage based on T-cell receptor gene, RNA, and protein studies: lineage does not predict clinical behavior

Nasal-type NK/T-cell lymphomas are more frequently T rather than NK lineage based on T-cell receptor gene, RNA, and protein studies: lineage does not predict clinical behavior

Transcription factors engaged in development of NK cells are commonly expressed in nasal NK/T-cell lymphomas

The Frequency of B- and T-Cell Gene Rearrangements and Epstein-Barr Virus in T-Cell Lymphomas

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