Detecting creatine excreted in the urine of swimming athletes by means of Raman spectroscopy

Moreira, Letícia Parada; Rocco, Débora Dias Ferraretto Moura; Silva, Alexandre Galvão da; Pacheco, Marcos Tadeu Tavares; Silveira, Landulfo

doi:10.1007/s10103-019-02843-z

Cited by 8 publications

(2 citation statements)

References 61 publications

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“…Raman spectroscopy has been used for serum analysis and diagnosis of different diseases such as endometriosis, Huntington’s disease, COVID-19, and cirrhosis [ 14 , 15 , 16 , 17 ]. The application of Raman spectroscopy for urine analysis has been previously studied to assist in early diagnosis by identifying biomarkers of nutritional supplementation, urinary tract infections, and other diseases, including chronic kidney disease and bladder cancer [ 18 , 19 , 20 , 21 , 22 , 23 , 24 ], the greatest advantage of Raman spectroscopy being the rapid analysis of individual samples. In short, Raman spectroscopy has been used to detect and quantify the main components in urine such as urea, creatinine, glucose, and phosphate [ 18 , 19 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

“…The application of Raman spectroscopy for urine analysis has been previously studied to assist in early diagnosis by identifying biomarkers of nutritional supplementation, urinary tract infections, and other diseases, including chronic kidney disease and bladder cancer [ 18 , 19 , 20 , 21 , 22 , 23 , 24 ], the greatest advantage of Raman spectroscopy being the rapid analysis of individual samples. In short, Raman spectroscopy has been used to detect and quantify the main components in urine such as urea, creatinine, glucose, and phosphate [ 18 , 19 , 21 , 22 ]. Phosphate is the most abundant intracellular anion within the body and is an important component that is present in multiple physiological processes that affect different organ systems [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

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Biochemical Analysis of Urine Samples from Diabetic and Hypertensive Patients without Renal Dysfunction Using Spectrophotometry and Raman Spectroscopy Techniques Aiming Classification and Diagnosis

et al. 2022

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The purpose of this study was to perform a comparative biochemical analysis between conventional spectrophotometry and Raman spectroscopy, techniques used for diagnoses, on the urine of healthy (CT) and diabetic and hypertensive patients (DM&HBP). Urine from 40 subjects (20 in the CT group and 20 in the DM&HBP group) was examined in a dispersive Raman spectrometer (an 830 nm excitation and a 350 mW power). The mean Raman spectra between both groups showed a significant difference in peaks of glucose; exploratory analysis by principal component analysis (PCA) identified spectral differences between the groups, with higher peaks of glucose and proteins in the DM&HBP group. A partial least squares (PLS) regression model estimated by the Raman data indicated the concentrations of urea, creatinine, glucose, phosphate, and total protein; creatinine and glucose were the biomarkers that presented the best correlation coefficient (r) between the two techniques analyzed (r = 0.68 and r = 0.98, respectively), both with eight latent variables (LVs) and a root mean square error of cross-validation (RMSecv) of 3.6 and 5.1 mmol/L (41 and 92 mg/dL), respectively. Discriminant analysis (PLS-DA) using the entire Raman spectra was able to differentiate the samples of the groups in the study, with a higher accuracy (81.5%) compared to the linear discriminant analysis (LDA) models using the concentration values of the spectrometric analysis (60.0%) and the concentrations predicted by the PLS regression (69.8%). Results indicated that spectral models based on PLS applied to Raman spectra may be used to distinguish subjects with diabetes and blood hypertension from healthy ones in urinalysis aimed at population screening.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biochemical Analysis of Urine Samples from Diabetic and Hypertensive Patients without Renal Dysfunction Using Spectrophotometry and Raman Spectroscopy Techniques Aiming Classification and Diagnosis

et al. 2022

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Raman Spectroscopic Analysis of Urinary Creatine and Phosphate in Athletes: Pre‐ and Post‐Training Assessment

Santos,

Silveira,

Pacheco

2024

Journal of Biophotonics

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The aim of this study was to detect biochemical components in the urine of bodybuilders who ingested creatine pretraining compared to individuals who did not ingest creatine after physical exercise using Raman spectroscopy. Twenty volunteers practicing bodybuilding were selected to collect pre‐ and post‐training urine samples, where 10 volunteers ingested creatine 30 min before pretraining urine collection (creatine group), and 10 did not (control group). The samples were subjected to Raman spectroscopy, and the spectra of both creatine and control groups and the difference (post—pre) for both groups were analyzed. Principal component analysis (PCA) technique was applied to the samples. The results showed peaks of creatine and phosphate in urine after training (creatine post‐training group), suggesting that part of the creatine was absorbed and metabolized, and part was excreted. Raman spectroscopy could be applied to detect biocompounds in urine, such as unmetabolized creatine and phosphate.

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Application of Raman Spectroscopy for the Evaluation of Metabolomic Dynamic Analysis in Athletic Horses

Acri

Testagrossa

Giudice

et al. 2021

Journal of Equine Veterinary Science

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Detecting creatine excreted in the urine of swimming athletes by means of Raman spectroscopy

Cited by 8 publications

References 61 publications

Biochemical Analysis of Urine Samples from Diabetic and Hypertensive Patients without Renal Dysfunction Using Spectrophotometry and Raman Spectroscopy Techniques Aiming Classification and Diagnosis

Biochemical Analysis of Urine Samples from Diabetic and Hypertensive Patients without Renal Dysfunction Using Spectrophotometry and Raman Spectroscopy Techniques Aiming Classification and Diagnosis

Raman Spectroscopic Analysis of Urinary Creatine and Phosphate in Athletes: Pre‐ and Post‐Training Assessment

Application of Raman Spectroscopy for the Evaluation of Metabolomic Dynamic Analysis in Athletic Horses

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