Detecting endotoxin activity in bovine serum using an automated testing system

Suzuki, Kazuyuki; Shimamori, Toshio; Sato, Azusa; Tsukano, Kenji; Tsuchiya, Masaharu; Lakritz, Jeffrey

doi:10.1292/jvms.14-0545

Cited by 3 publications

(2 citation statements)

References 5 publications

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“…Endotoxin activities in plasma and rumen fluid were measured by conventional limulus amebocyte lysate (LAL)-kinetic turbidimetric (KTA) and chromogenic assays, respectively. Immediately before testing, plasma and rumen samples were diluted 20-and 10,000-fold in endotoxin-free water (Otsuka distilled water, Otsuka Pharmaceutical Co., Ltd., Tokyo, Japan), respectively, and the dilutions were vortexed for 10 s. Plasma dilutions were then heated for 10 min at 80°C to inactivate interfering substances, such as protease inhibitors (Suzuki et al, 2015). The diluted rumen fluids were used for analysis without heating.…”

Section: Blood Plasma and Serum Concentrationsmentioning

confidence: 99%

Effects of butyrate supplementation on blood glucagon-like peptide-2 concentration and gastrointestinal functions of lactating dairy cows fed diets differing in starch content

Fukumori

Oba

Izumi

et al. 2020

Journal of Dairy Science

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The objective of this study was to evaluate effects of butyrate supplementation on plasma concentration of glucagon-like peptide-2 (GLP-2), apparent total-tract digestibility, and responses to a grain challenge of lactating dairy cows fed diets differing in starch content. Eight Holstein cows averaging 58.6 ± 9.96 d in milk (4 primiparous cows fitted with rumen cannula and 4 multiparous intact cows) were blocked by parity and assigned to one of two 4 × 4 Latin squares balanced for carryover effects with a 2 × 2 factorial arrangement of treatments. Treatments were dietary starch content [20.6 vs. 27.5%, respectively, for low starch (LS) and high starch (HS)] and butyrate supplementation (butyrate vs. control) with 21-d periods. Butyrate was provided as Gustor BP70 WS (Norel, S.A., Madrid, Spain), containing 70% sodium butyrate and 30% fatty acid mixture, at 2% of dietary dry matter (providing butyrate at 1.1% of dietary dry matter), and control premix contained 70% wheat bran and 30% fatty acid mixture. Feeds, orts, and fecal samples were collected from d 17 to 19 to determine apparent total-tract nutrient digestibility. Blood and rumen fluid samples were collected on d 19. The baseline of dry matter intake (DMI) was determined as average DMI from d 17 to 19 for each cow, and cows were feed-restricted at 60% of the baseline DMI on d 20, and a grain challenge was conducted by providing steam-flaked corn grain at 0.6% of body weight, on an as-fed basis, in addition to each treatment diet on d 21, and blood and ruminal fluid samples were collected. The interaction of dietary starch content by butyrate supplementation was significant for plasma GLP-2 concentration, being greater for cows fed butyrate with the HS diet than those fed the other 3 diets. Cows fed butyrate increased nbutyrate concentration in the ruminal fluid and tended to increase dry matter and organic matter digestibility compared with the control. During the grain challenge, rumen endotoxin concentration increased over time and was higher for cows fed the HS diets compared with those fed LS diets. However, response variables related to inflammation were not affected by the grain challenge. However, serum haptoglobin, lipopolysaccharidebinding protein, and serum amyloid-A concentrations were greater for cows fed butyrate with the LS diet, but not for those fed the HS diet. These results indicate that butyrate supplementation may increase plasma GLP-2 concentration for cows fed HS diets, and totaltract digestibility regardless of dietary starch content. However, butyrate supplementation did not mitigate inflammation in this study.

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Section: Blood Plasma and Serum Concentrationsmentioning

confidence: 99%

Effects of butyrate supplementation on blood glucagon-like peptide-2 concentration and gastrointestinal functions of lactating dairy cows fed diets differing in starch content

Fukumori

Oba

Izumi

et al. 2020

Journal of Dairy Science

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“…Immediately prior to testing, plasma and BALF samples were diluted 20- and 100-fold in endotoxin-free water (Otsuka distilled water, Otsuka Pharmaceutical Co., Ltd., Tokyo, Japan), respectively, and the dilutions were vortexed for 10 sec. The plasma dilutions were then heated for 10 min at 80°C in order to inactivate interfering substances, such as protease inhibitors [21]. BALF specimens were used for analysis without heating.…”

mentioning

confidence: 99%

Relationship between bronchoalveolar lavage fluid and plasma endotoxin activity in calves with bronchopneumonia

Nishi

Tsukano

Otsuka

et al. 2019

The Journal of Veterinary Medical Science

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The aim of this study was to investigate the relationship between the endotoxin activity in plasma and that in bronchoalveolar lavage fluid (BALF) in bronchopneumonia. Thirty-three calves were included in this study (17 healthy calves and 16 calves with respiratory disease). In the calves with bronchopneumonia, the median endotoxin activity in plasma (0.437 EU/m l , P <0.001) and BALF (29.45 EU/m l , P <0.001) was significantly higher than in the control calves. Plasma endotoxin activity was significantly and positively correlated with that in BALF ( r 2 =0.900, P <0.001). Based on the receiver operating characteristics curves, we propose a diagnostic cutoff point for plasma endotoxin activity (0.104 EU/m l , AUC=0.914, P <0.001, Se 81.3% and Sp 82.4%) for identification of bronchopneumonia in calves which could die within a week.

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Research on Graphic User Interface Automation Testing Technology Based on Cloud Platform

Liu

2019

J. Phys.: Conf. Ser.

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As the soul of computer, software has more and more important influence on human beings. GUI (Graphical User Interface) technology is widely used because of its friendly interface and easy operation. It is an important part of software nowadays. No matter what kind of software, from requirement analysis to detailed code design stage, we need to use excellent testing technology to lay the foundation for the smooth development of software. Software testing technology has become an important branch affecting the development of software industry. Especially in the regression test conducted in the later stage of the test, it is necessary to verify whether the previously discovered problem is solved in the new version. Automating software testing can make a large number of tests programmatically repeated. Not only does it save a lot of labor, but it also improves test efficiency and guarantees the quality of the test. This paper proposes a graphical user interface automation test technology for cloud platforms, which provides assurance for testers to easily create and flexibly maintain test code.

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Detecting endotoxin activity in bovine serum using an automated testing system

Cited by 3 publications

References 5 publications

Effects of butyrate supplementation on blood glucagon-like peptide-2 concentration and gastrointestinal functions of lactating dairy cows fed diets differing in starch content

Effects of butyrate supplementation on blood glucagon-like peptide-2 concentration and gastrointestinal functions of lactating dairy cows fed diets differing in starch content

Relationship between bronchoalveolar lavage fluid and plasma endotoxin activity in calves with bronchopneumonia

Research on Graphic User Interface Automation Testing Technology Based on Cloud Platform

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