Detecting Envelope Stress by Monitoring β-Barrel Assembly

Cho, Seung-Hyun; Szewczyk, Joanna; Pesavento, Christina; Zietek, Matylda; Banzhaf, Manuel; Roszczenko, Paula; Asmar, Abir T.; Laloux, Géraldine; Hov, Ann-Kristin; Leverrier, Pauline; Henst, Charles Van der; Vertommen, Didier; Typas, Athanasios; Collet, Jean-François

doi:10.1016/j.cell.2014.11.045

Cited by 157 publications

(299 citation statements)

References 58 publications

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“…A22 inhibits the actinlike MreB protein and provides superior activation of the Rcs phosphorelay compared with bile salts (Fig. S4B) (45,50). As expected from our previous results ( induction and did not up-regulate RicI (Fig.…”

Section: Resultssupporting

confidence: 85%

“…Because the Rcs system strongly induces the rprA promoter in E. coli (21), and other cell envelope-damaging conditions trigger RprA synthesis through RcsB (21,(43)(44)(45), we hypothesized that the bile-induced increase in RicI synthesis is indirect, resulting from the Rcs-mediated activation of RprA. To test this, we constructed single-gene deletion strains of various components of the Rcs signaling cascade and evaluated bile-induced changes in the levels of RprA and RicI levels in these mutants.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Small RNA-based feedforward loop with AND-gate logic regulates extrachromosomal DNA transfer in Salmonella

Papenfort

Espinosa

Casadesús

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Horizontal gene transfer via plasmid conjugation is a major driving force in microbial evolution but constitutes a complex process that requires synchronization with the physiological state of the host bacteria. Although several host transcription factors are known to regulate plasmid-borne transfer genes, RNA-based regulatory circuits for host-plasmid communication remain unknown. We describe a posttranscriptional mechanism whereby the Hfq-dependent small RNA, RprA, inhibits transfer of pSLT, the virulence plasmid of Salmonella enterica. RprA employs two separate seed-pairing domains to activate the mRNAs of both the sigma-factor σ S and the RicI protein, a previously uncharacterized membrane protein here shown to inhibit conjugation. Transcription of ricI requires σ S and, together, RprA and σ S orchestrate a coherent feedforward loop with AND-gate logic to tightly control the activation of RicI synthesis. RicI interacts with the conjugation apparatus protein TraV and limits plasmid transfer under membrane-damaging conditions. To our knowledge, this study reports the first small RNA-controlled feedforward loop relying on posttranscriptional activation of two independent targets and an unexpected role of the conserved RprA small RNA in controlling extrachromosomal DNA transfer.RprA | sRNA | feedforward control | plasmid conjugation | Hfq

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Section: Resultssupporting

confidence: 85%

Section: Resultsmentioning

confidence: 99%

Small RNA-based feedforward loop with AND-gate logic regulates extrachromosomal DNA transfer in Salmonella

Papenfort

Espinosa

Casadesús

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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“…The 10× concentrated medium was supplemented with 10% FCS before use. Mass spectrometry was performed as described (Cho et al, 2014).…”

Section: Embryonic Endothelial Progenitor Cell-conditioned Medium (Eementioning

confidence: 99%

Thyroid follicle development requires Smad1/Smad5- and endothelial-dependent basement membrane assembly

et al. 2016

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Thyroid follicles, the functional units of the thyroid gland, are delineated by a monolayer of thyrocytes resting on a continuous basement membrane. The developmental mechanisms of folliculogenesis, whereby follicles are formed by the reorganization of a non-structured mass of non-polarized epithelial cells, are largely unknown. Here we show that assembly of the epithelial basement membrane is crucial for folliculogenesis and is controlled by endothelial cell invasion and by BMP-Smad signaling in thyrocytes. Thyroid-specific Smad1 and Smad5 double-knockout (Smad1/5 dKO ) mice displayed growth retardation, hypothyroidism and defective follicular architecture. In Smad1/5 dKO embryonic thyroids, epithelial cells remained associated in large clusters and formed small follicles. Although similar follicular defects are found in Vegfa knockout (Vegfa KO ) thyroids, Smad1/5 dKO thyroids had normal endothelial cell density yet impaired endothelial differentiation. Interestingly, both Vegfa KO and Smad1/5 dKO thyroids displayed impaired basement membrane assembly. Furthermore, conditioned medium (CM) from embryonic endothelial progenitor cells (eEPCs) rescued the folliculogenesis defects of both Smad1/5 dKO and Vegfa KO thyroids. Laminin α1, β1 and γ1, abundantly released by eEPCs into CM, were crucial for folliculogenesis. Thus, epithelial Smad signaling and endothelial cell invasion promote folliculogenesis via assembly of the basement membrane.

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“…Intriguingly, signal sensing mediated by RcsF involves a switch in its localization. RcsF is exposed to the cell surface by the outer membrane ␤-barrel protein assembly machinery, but upon perturbation of outer membrane protein assembly or lipoprotein transport machinery, newly synthesized RcsF is no longer surface-exposed and is then able to trigger Rcs signaling from the periplasm via IgaA (7).…”

mentioning

confidence: 99%

Interaction of the RcsB Response Regulator with Auxiliary Transcription Regulators in Escherichia coli

Pannen

Fabisch

Gausling

et al. 2016

Journal of Biological Chemistry

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The Rcs phosphorelay is a two-component signal transduction system that is induced by cell envelope stress. RcsB, the response regulator of this signaling system, is a pleiotropic transcription regulator, which is involved in the control of various stress responses, cell division, motility, and biofilm formation. RcsB regulates transcription either as a homodimer or together with auxiliary regulators, such as RcsA, BglJ, and GadE in Escherichia coli. In this study, we show that RcsB in addition forms heterodimers with MatA (also known as EcpR) and with DctR. Our data suggest that the MatA-dependent transcription regulation is mediated by the MatA-RcsB heterodimer and is independent of RcsB phosphorylation. Furthermore, we analyzed the relevance of amino acid residues of the active quintet of conserved residues, and of surface-exposed residues for activity of RcsB. The data suggest that the activity of the phosphorylation-dependent dimers, such as RcsA-RcsB and RcsB-RcsB, is affected by mutation of residues in the vicinity of the phosphorylation site, suggesting that a phosphorylation-induced structural change modulates their activity. In contrast, the phosphorylation-independent heterodimers BglJ-RcsB and MatA-RcsB are affected by only very few mutations. Heterodimerization of RcsB with various auxiliary regulators and their differential dependence on phosphorylation add an additional level of control to the Rcs system that is operating at the output level.

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Detecting Envelope Stress by Monitoring β-Barrel Assembly

Cited by 157 publications

References 58 publications

Small RNA-based feedforward loop with AND-gate logic regulates extrachromosomal DNA transfer in Salmonella

Small RNA-based feedforward loop with AND-gate logic regulates extrachromosomal DNA transfer in Salmonella

Thyroid follicle development requires Smad1/Smad5- and endothelial-dependent basement membrane assembly

Interaction of the RcsB Response Regulator with Auxiliary Transcription Regulators in Escherichia coli

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