Detecting local immunoglobulin E from mucosal brush biopsy of the inferior turbinates using microarray analysis

Reisacher, William R.

doi:10.1002/alr.21111

Cited by 16 publications

(10 citation statements)

References 24 publications

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“…Seven common airborne allergens and 1 food allergen were chosen for this study: White oak, Timothy grass, ragweed, cat, Alternaria , Dermatophagoides farinae , cockroach, and peanut. A food allergen was included because species‐specific IgE to food allergens have previously been identified in the nasal cavity using MBB . The actual sensitization of the participants to these allergens, based on either serum IgE testing, skin testing, or local IgE testing, was unknown prior to enrollment.…”

Section: Methodsmentioning

confidence: 99%

Total and allergen‐specific immunoglobulin E in the serum and nasal mucosa of a nonallergic population

Reisacher

2016

Int Forum Allergy Rhinol

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Section: Methodsmentioning

confidence: 99%

Total and allergen‐specific immunoglobulin E in the serum and nasal mucosa of a nonallergic population

Reisacher

2016

Int Forum Allergy Rhinol

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“…MBB testing in other regions of the oral cavity and GI tract should also be explored. Using microarray analysis of MBB samples from the inferior turbinate, species‐specific IgE to shrimp was detected . Future studies will also determine whether or not airborne allergens can be detected from oral cavity MBB testing.…”

Section: Discussionmentioning

confidence: 99%

Mucosal brush biopsy of the oral cavity to detect local, peanut‐specific immunoglobulin E

Reisacher

Cohen

2013

Int Forum Allergy Rhinol

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This study demonstrates for the first time that peanut-specific IgE can be detected using MBB in the oral cavity of patients who are symptomatic when consuming peanuts. The vestibule was a superior location compared to the dorsal tongue for oral cavity MBB, correlating very well with self-reported symptoms. Peanut-specific IgE on MBB overall did not correlate well with serum testing for peanut-specific IgE.

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“…We are prompted to speculate that the IgE repertoire of NS of young infants with cow milk, egg, or other food allergies is mostly dominated by those animal food allergens. The presence of IgE to animal foods has been already described in the nasal mucosa, 28 but this aspect remains quite open for further investigations. partly be due to the relatively small study population size, 11 to the diversity in collection and procession methods, 1 or the sensitivity of the IgE assays.…”

Section: Ige Repertoire In Ns and In Serummentioning

confidence: 95%

IgE antibody repertoire in nasal secretions of children and adults with seasonal allergic rhinitis: A molecular analysis

Arasi

Tripodi

et al. 2020

Pediatric Allergy Immunology

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Background: There is growing interest both in testing IgE in nasal secretions (NS) and in molecular diagnosis of seasonal allergic rhinitis (SAR). Yet, the reliability of nasal IgE detection with the newest molecular assays has never been assessed in a large cohort of pollen allergic patients.Objective: To investigate with microarray technology and compare the repertoires of specific IgE (sIgE) antibodies in NS and sera of a large population of children and adults with SAR.Methods: Nasal secretions were collected with an absorbent device (Merocel 2000 ® , Medtronic) and a minimal dilution procedure from 90 children and 71 adults with SAR. Total IgE (tIgE) (ImmunoCAP, Thermo Fisher Scientific (TFS)) and sIgE antibodies against 112 allergen molecules (ISAC-112, TFS) were measured in NS and serum.Results: Nasal sIgE was detectable in 68.3% of the patients. The detected nasal sIgE antibodies recognized airborne (88%), vegetable (10%), and animal food or other (<1%) allergen molecules. The prevalence and average levels of sIgE in NS and serum were highly interrelated at population level. A positive nasal sIgE antibody to a given molecule predicted the detection of the same antibody in the patient's serum with a specificity of 99.7% and a sensitivity of 40%. Conclusions:The concentration of sIgE is much lower in nasal secretions than in the serum. sIgE assays with very high analytical sensitivity and sampling methods with minimal dilution will be therefore needed to validate nasal secretions as alternative to serum in testing the sIgE repertoire.This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Detecting local immunoglobulin E from mucosal brush biopsy of the inferior turbinates using microarray analysis

Abstract: This is the first demonstration that antigen-specific IgE in saline samples can be measured using MA. The ability of MA to measure smaller amounts of IgE, with similar accuracy, may give it a potential advantage for MBB analysis in the future.

Cited by 16 publications

References 24 publications

Total and allergen‐specific immunoglobulin E in the serum and nasal mucosa of a nonallergic population

Total and allergen‐specific immunoglobulin E in the serum and nasal mucosa of a nonallergic population

Mucosal brush biopsy of the oral cavity to detect local, peanut‐specific immunoglobulin E

IgE antibody repertoire in nasal secretions of children and adults with seasonal allergic rhinitis: A molecular analysis

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