Detecting Swelling States of Red Blood Cells by “Cell–Fluid Coupling Spectroscopy”

Zensen, Carla; Fernandez, Isis E.; Eickelberg, Oliver; Feldmann, Jochen; Lohmüller, Theobald

doi:10.1002/advs.201600238

Cited by 3 publications

(1 citation statement)

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“…By utilizing a single gold nanoparticle optically trapped in water and processing video sequences of its motion in the frequency domain, it was possible to detect faint acoustic vibrations at sound power levels down to −60 dB; more than six orders of magnitude more sensitive than the human ear. [ 108 ] This approach was later expanded upon and employed to monitor the rotation frequency of flagellar bundles of single bacteria cells, [ 109 ] the swelling state of red blood cells, [ 110 ] and the fitness of aquatic nauplius larvae. [ 111 ] Further, by introducing a quasi “lock‐in” approach for particle tracking and analyzing the particle motion in the frequency domain, the sensitivity of force measurements with an optically trapped gold particle could be shifted to the fN range.…”

Section: Mainmentioning

confidence: 99%

Plasmonic Nanoagents in Biophysics and Biomedicine

Huergo

Schuknecht

Zhang

et al. 2022

Advanced Optical Materials

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the context of theranostics [1] (a portman teau of the words therapeutics and dia gnostics). Here, they have been employed for in vivo diagnostics and subsequent treatment of cancer, [2] and the protection against viral infections. [3] The possibilities for the design of nanoagents are vast. [4] Examples include, but are not limited to multifunctional nanocarriers based on organic molecules and nanoparticles, [5] metal based nanoagents, [6] carbon dots, [7] radiolabeled probes, [8] photoswitchable systems, [9] as well as hybrid bioactive materials and frameworks [10] and smart substrates that interact specifically with living cells. [11] Amongst these many intriguing examples, plasmonic nano particles and nanosystems distinguish themselves as nanoagents by three main features that arise from their optical properties: They are highly susceptible environmental sensors, they have superior applica bility as nanoscale sources of heat, and they can be moved in a controlled manner by means of light. It is the combination of these properties, that paves the way for the development of plasmonic nanoagents towards applications in biophysics and biomedicine. [12] The aim of this article is to review a seminal selection of strategies for the design and development of plasmonic nano agents to manipulate and monitor biological functions. For this, we begin with a brief overview of the physical mechanisms gov erning plasmonic nanoparticles. We continue with the evolution of plasmonic sensing from single biomolecule detection towards in vivo applications. Here, to keep the article concise, we restrict ourselves to sensing applications that do not involve surface enhanced Raman scattering (SERS) spectroscopy, [13] although we acknowledge that SERS has been widely applied in biological studies [14] and plays an important role in medical applications such as cancer imaging and treatment, as well. [15] Second, we discuss the potential of lightcontrolled plasmonic heating, for manipulating biomolecular systems in combination with sensing. Finally, we highlight examples of plasmonic nanosys tems that take advantage of a combination of sensing, tempera ture control as well as optical forces, through which plasmonic particles unfold their full potential as nanoagents. This leads us to an outlook on the future of plasmonic nanoagents as advanced optical materials in biophysics and biomedicine. MainThe optical properties of plasmonic nanoparticles are covered in great detail in numerous review articles [16] and textbooks. [17] The significant rise in implementation and applications of plasmonic nanosystems in biophysics, biochemistry, and medicine has culminated in the emergence of refined plasmonic enabling reagents, or "nanoagents". These are defined as tools that allow researchers to not only investigate, but also actively manipulate biological processes and complex biosystems, such as living cells, on the nanoscale. This development is based on a combination of sensing capabilities, photothermal control, and optical force manipul...

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