Detecting the Enterotoxigenicity of
            <i>Staphylococcus aureus</i>
            Strains

Robbins, Ruth N.; Gould, Sara; Bergdoll, Merlin S.

doi:10.1128/am.28.6.946-950.1974

Cited by 112 publications

(7 citation statements)

References 8 publications

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“…To visualize the ability of producing SEA of the 109 selected cultures, OSP (Optimum Sensitivity Plate) immunodifusion method was used (ROBBINS et al, 1974). The immunodifusion medium was prepared with 8.5g of NaCl, 5.0 mL of mertiolate solution at 2% and 12.0 g of high purity agar dissolved in 1000 mL of Tris buffer 0.05M, ph 7.4.…”

Section: Methodsmentioning

confidence: 99%

Determinação Do Risco De Consumo De Leite Cru Relacionado À Intoxicação Estafilocócica

et al. 2010

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This study aimed to identify the risks of staphylococcal food poisoning due to the consumption of raw milk. Fifty-one farms in Londrina (PR) and 50 in Pelotas (RS) were analyzed, to determine the population of coagulase-positive staphylococci (UFC/ mL), as well as to verify the ability of producing Staphylococcal Enterotoxin A (SEA) by immunodifusion (OSP), the presence of the gene for the production of SEA (PCR) in the cultures, and the research of enterotoxin (SEA to SEE) in milk samples using ELISA commercial kit. Considering the 101 farms analyzed, 19 (18.8%) presented coagulase-positive staphylococci count above 105 UFC/mL. For the evaluation of the enterotoxigenic ability (SEA) by the OSP technique, six cultures coagulase-positive (5.5%) were positive to the test and identified as S. aureus. From the coagualse-negative sample, one (5.5%) was OSP positive. For the evaluation of the presence of the gene for EEA synthesis, 51 cultures of staphylococci were tested. From this total, 14 (27.45%)

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Section: Methodsmentioning

confidence: 99%

Determinação Do Risco De Consumo De Leite Cru Relacionado À Intoxicação Estafilocócica

et al. 2010

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“…Immunodiffusion assay. Gel double-immunodiffusion assays of rTSST-1 and mTSST-1 with anti-TSST-1 antibody were done basically according to the method described by Robbins et al [41], with minor modifications. Noble agar (Difco), 1.2% in 0.01 mol/L PBS, which contained 10 ppm thimerosal, was poured into plastic petri dishes.…”

Section: Methodsmentioning

confidence: 99%

Vaccination with Nontoxic Mutant Toxic Shock Syndrome Toxin 1 Protects againstStaphylococcus aureusInfection

Hu¹,

Omoe²,

Sasaki³

et al. 2003

J INFECT DIS

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To investigate whether vaccination with nontoxic mutant toxic shock syndrome toxin 1 (mTSST-1) can protect against Staphylococcus aureus infection, mice were vaccinated with mTSST-1 and challenged with viable S. aureus. Survival in the mTSST-1-vaccinated group was higher, and bacterial counts in organs were significantly lower than those of control mice. Passive transfer of mTSST-1-specific antibodies also provided protection against S. aureus-induced septic death. Interferon (IFN)-gamma production in the serum samples and spleens from vaccinated mice was significantly decreased compared with that in controls, whereas interleukin-10 titers were significantly higher in vaccinated mice. IFN-gamma and tumor necrosis factor-alpha production in vitro were significantly inhibited by serum samples from mTSST-1-immunized mice but not from control mice. These results suggest that vaccination with mTSST-1 devoid of superantigenic properties provides protection against S. aureus infection and that the protection might be mediated by TSST-1-neutralizing antibodies as well as by the down-regulation of IFN-gamma production.

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“…Serological assays. Western blot (immunoblot) analysis and gel double-diffusion assays were performed as described previously (27,50). Gel double-diffusion-grade SEA and rabbit polyclonal anti-SEA and anti-SEB antisera were obtained from Merlin Bergdoll.…”

Section: Methodsmentioning

confidence: 99%

Lack of complete correlation between emetic and T-cell-stimulatory activities of staphylococcal enterotoxins

et al. 1993

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This study examined the emetic activity of several staphylococcal enterotoxin type A and B (SEA and SEB, respectively) mutants that had either one or two amino acid residue substitutions. New sea gene mutations were constructed by site-directed mutagenesis; gene products were obtained with glycine residues at position 25, 47, 48, 81, 85, or 86 of mature SEA. Culture supernatants from Staphylococcus aureus RN4220, or derivatives containing either sea or a sea mutation, were analyzed for the ability to stimulate proliferation of murine splenocytes, as determined by incorporation of [3H]thymidine. Culture supernatants containing SEA-N25G (a SEA mutant with a substitution of glycine for the asparagine residue at position 25), SEA-F47G, or SEA-L48G did not stimulate T-cell proliferation, unlike supernatants containing the other substitution mutants. Purified preparations of SEA-N25G had weak activity and those of SEA-F47G and SEA-L48G had essentially no activity in the T-cell proliferation assay. All mutants except SEA-V85G, which was degraded by monkey stomach lavage fluid in vitro, were tested for emetic activity. SEA-C106A and two SEB mutants, SEB-D9N/N23D and SEB-F44S (previously referred to as BR-257 and BR-358, respectively), whose construction and altered immunological properties have been reported previously, were also tested in the emetic assay. Each mutant was initially administered intragastrically at doses of 75 to 100 micrograms per animal; if none of the animals responded, the dose was increased four-to fivefold. SEA-F47G, SEA-C106A, and SEB-D9N/N23D were the only mutants that did not induce vomiting at either dose tested; these three mutants had reduced immunological activity. However, there was not a perfect correlation between immunological and emetic activities; SEA-L48G and SEB-F44S retained emetic activity, although they had essentially no T-cell-stimulatory activity. These studies suggest that these two activities can be dissociated.

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Detecting the Enterotoxigenicity of Staphylococcus aureus Strains

Cited by 112 publications

References 8 publications

Determinação Do Risco De Consumo De Leite Cru Relacionado À Intoxicação Estafilocócica

Determinação Do Risco De Consumo De Leite Cru Relacionado À Intoxicação Estafilocócica

Vaccination with Nontoxic Mutant Toxic Shock Syndrome Toxin 1 Protects againstStaphylococcus aureusInfection

Lack of complete correlation between emetic and T-cell-stimulatory activities of staphylococcal enterotoxins

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