Detecting the Enterotoxigenicity of Staphylococcus aureus Strains

Abstract

An optimal sensitivity plate method for examining large numbers of staphylococcal strains for production of the known enterotoxins (A-E) is presented. Small volumes of relatively concentrated enterotoxin are produced by the semi-solid agar, cellophane-over-agar, or sac culture techniques. Detection of the enterotoxin in the supernatant fluid is accomplished with the optimal sensitivity plate method. In this method small plastic petri dishes (50 mm) were used for a modified Ouchterlony of high sensitivity.

Enterotoxigenic staphylococci in Sri Lanka

“…Hallander & Korlof (1967) found some evidence that enterotoxin production may be related to clinical virulence of strains. A similar view has been expressed by Robbins et al (1974). Five serologically distinct enterotoxins designated by the letters A-E are now recognized.…”

Efficacy of bovicin HC5 and nisin combination against Listeria monocytogenes and Staphylococcus aureus in fresh cheese

“…After the confirmation of S. aureus cultures identity by biochemical tests, they were transferred to tubes containing 5 mL of BHI and incubated at 37°C for 24 h. The staphylococcal enterotoxin (SE) assay was performed at the Laboratory of Staphylococcal Enterotoxins, FUNED (Belo Horizonte, MG, Brazil), using the Optimum Sensitivity Plating as described by Robbins et al (1974).…”

“…The solution was centrifuged again at 10 000 g for 20 min and after filtration, three drops of the preservative thimerosol were added. Next, the identification test of staphylococcal enterotoxin was performed by the immuno-gel diffusion method, optimum sensitive plate (OSP) (Robbins et al, 1974).…”

Frequency of nasal and wound isolates of Staphylococcus aureus associated with TSST-1 production in Jordanian population

“…Presence of TSST-1 in culture supernatants was detected by two methods: (a) immunodi¡usion optimal sensitivity plate assay (OSP) was performed according to the method of Robbin et al [14]; (b) noncompetitive enzyme-linked immunosorbent assay (ELISA).…”