Detection and analysis of nucleic acid in various biological samples of COVID-19 patients

Wu, Jianguo; JiaSheng, Liu; Li, Shijun; Peng, Zhiyang; Xiao, Zhe; Wang, Xufeng; Yan, R.H.; Luo, Jin-Yan

doi:10.1016/j.tmaid.2020.101673

Cited by 184 publications

(191 citation statements)

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“…Interestingly, the percentage of positive anal swabs in the cured patients was much higher than the positive pharyngeal swabs. PCR positivity of anal swabs was reported in several studies, which has led to a discussion on the possibility of fecal-oral transmission [12,13]. The reason for PCR positive anal swabs may be that the virus enters the digestive tract from the patient's mouth.…”

Section: Discussionmentioning

confidence: 98%

No evidence of Re-infection or Human-to-Human Transmission in Cured COVID-19 Patients, a Retrospective Cohort Study

Liu

Zhao

et al. 2020

Preprint

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Objective: To clarify the clinical features of cured patients with coronavirus disease (COVID-19) and the relevance of IgM and IgG testing.Methods: A total of 187 cured COVID-19 patients with antibody test were followed up every two weeks at Guangzhou Eighth People's hospital. Assessment for general condition, symptoms, epidemiological contact history, polymerase chain reaction (PCR) assay, and antibody tests were performed and recorded. Information from Guangzhou CDC was also screened.Results: There were 154 (82.4%) patients with positive results for IgG and 35 (18.7%) patients with positive results for IgM. PCR assay was positive in 10 (5.3%) patients. Neither IgG nor IgM results showed a relationship with PCR test results (all P > 0.05). No re-infection was found in the cured patients. Among people who were in close contact with the cured patients, no one was diagnosed with COVID-19 as reported both by the cured patients and the Guangzhou CDC. Factors associated with appearance of IgG comprised hospitalization days (OR: 1.07, 95%CI: 1.02-1.13, P = 0.004) and antibiotics treatment (OR: 2.78, 95%CI: 1.10-7.01, P = 0.031) .Conclusion: In our study, neither re-infection nor human-to-human transmission was found in cured patients with COVID-19. Additionally, neither IgG nor IgM can be used to replace the PCR test in cured patients.

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Section: Discussionmentioning

confidence: 98%

No evidence of Re-infection or Human-to-Human Transmission in Cured COVID-19 Patients, a Retrospective Cohort Study

Liu

Zhao

et al. 2020

Preprint

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“…Two consecutive negative RNA tests is recognized as one of the discharge criteria [9]. According to the report of Wu et al, the positive rate of the SARS-CoV-2 RT-PCT test of nasopharyngeal swab, sputum, blood, feces and anal swabs is 38.13%, 48.68%, 3.03%, 9.83% and 10.00%, respectively [21]. Xiao et al reported that 21.4% of patients presented with a positive virus RNA test again after two consecutive negative tests.…”

Section: Discussionmentioning

confidence: 99%

Clinical Characteristics of COVID-19 Patients with Recurrent PCR Positivity After Hospital Discharge

Wang

Tian

2020

Preprint

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Objective: This study aimed to investigate the clinical characteristics of COVID-19 patients with recurrent SARS-CoV-2 positivity after hospital discharge. Methods: This retrospective study included COVID-19 patients who were readmitted for recurrence of positive SARS-CoV-2 RNA. Univariate and multivariate analyses were performed to assess the risk factors associated to the duration of recurrent RNA positivity.Results: Among the 287 discharged COVID-19 patients, 33 (11.5%) patients with recurrent PCR positivity were included. Among these patients, 21 (63.7%) patients were female, their mean age was 48.7 (±19.7) years old. 22 (66.7%) patients were asymptomatic. The following clinical features were presented in other patients: cough, fatigue, sore throat, fever and expectoration. The chest CT findings revealed that 8 (24.2%) patients were characterized by deterioration compared to the previous results. The median duration of recurrent RNA positivity was 9.0 days (IQR, 6.0, 15.0). We found that increased serum SARS-CoV-2-specific IgG antibody titer, elevated serum creatinine level, and female gender were the risk factors for the prolonged duration of recurrent RNA positivity.Conclusion: SARS-CoV-2 turned positive in a minority of discharged patients with COVID-19. Most patients experienced mild clinical course. Increased IgG antibody titer, creatinine and female gender were correlated to the prolonged RNA clearance time.

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“…were collected simultaneously in our study. Some studies reported that sputum specimen has a higher viral load and should be collected if possible [23]. A recent study reported that SARS-CoV-2 can be detected in the self-collected saliva of 91.7% (11/12) of patients and live virus was detected in saliva, suggesting that saliva is a promising noninvasive specimen for diagnosis in patients with 2019-nCoV infection [24].…”

Section: Discussionmentioning

confidence: 99%

Optimized Laboratory Detection Strategy for COVID-19 Patients Reduces the Rate of Missed Diagnosis

Chang

Shi

et al. 2020

Preprint

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Background: Novel coronavirus pneumonia (NCP) is an emerging, highly contagious community acquired pneumonia (CAP) caused by severe acute SARS-CoV-2. Nucleic acid test currently played a crucial role in diagnosis of suspected COVID-19 patients. However, a high false-negative rate of this “gold standard” test has been reported and posed a major setback in blocking the spread of the virus. We here aim to describe an optimized laboratory detection strategy to reduce the false negative rate. Methods: Suspected NCP patients were asked to collect both coughed up specimen and pharyngeal swab. Samples from the same patient were mixed and tested at a single pool. SARS-CoV-2 was then detected by real-time RT-PCR using two different detection kits. Only if both results were negative was the test reported as negative. The patients will be excluded after two consecutive negative tests at 24 hour intervals. We also used multiplex PCR to detect 13 common respiratory tract pathogens (RTP). Results: Using this strategy, we confirmed 85 SARS-CoV-2 infections from 181 suspected patients, and 94.12% of patients were positive in the first test. The 96 excluded patients were followed up, and no additional NCP was found. We also found that 31.25% patients in 96 non-NCP patients were infected with at least one RTP that may cause CAP. Conclusion: Our studies suggest that dual reagents screening with pooled coughed up specimen and pharyngeal swab samples reduced the false negative rate of nucleic acid testing. During the epidemic of NCP in Anhui province, there was a certain proportion of infection and co-infection of other common pathogens of CAP. In comparison with SARS-CoV-2 detection alone, combining multiple pathogen detection reduces the rate of miss diagnosis.

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Detection and analysis of nucleic acid in various biological samples of COVID-19 patients

Cited by 184 publications

References 1 publication

No evidence of Re-infection or Human-to-Human Transmission in Cured COVID-19 Patients, a Retrospective Cohort Study

No evidence of Re-infection or Human-to-Human Transmission in Cured COVID-19 Patients, a Retrospective Cohort Study

Clinical Characteristics of COVID-19 Patients with Recurrent PCR Positivity After Hospital Discharge

Optimized Laboratory Detection Strategy for COVID-19 Patients Reduces the Rate of Missed Diagnosis

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Detection and analysis of nucleic acid in various biological samples of COVID-19 patients

Cited by 184 publications

References 1 publication

No evidence of Re-infection or&nbsp;Human-to-Human Transmission&nbsp;in Cured COVID-19 Patients, a Retrospective Cohort Study

No evidence of Re-infection or&nbsp;Human-to-Human Transmission&nbsp;in Cured COVID-19 Patients, a Retrospective Cohort Study

Clinical Characteristics of COVID-19 Patients with Recurrent PCR Positivity After Hospital Discharge

Optimized Laboratory Detection Strategy for COVID-19 Patients Reduces the Rate of Missed Diagnosis

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Product

Resources

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No evidence of Re-infection or Human-to-Human Transmission in Cured COVID-19 Patients, a Retrospective Cohort Study

No evidence of Re-infection or Human-to-Human Transmission in Cured COVID-19 Patients, a Retrospective Cohort Study