Detection and Analysis of the Oral Flora in Patients with Recurrent Aphthous Stomatitis

Abstract

The present study detected differences in the oral mucosal flora in healthy people and patients diagnosed with recurrent aphthous stomatitis (RAS) using the 16S ribosomal RNA high-throughput sequencing (rRNA-seq). All samples were collected from the lower lip mucosa of 100 healthy individuals and 100 patients with RAS. After the extraction, DNA was subjected to PCR amplification for the bacterial 16S rRNA gene, before subjecting to high-throughput sequencing, and matched to a database. Most bacterial species and most unique bacteria were from the healthy control group, and the amount of operational taxonomic units (OTUs) calculated was similar in the ulcer and nonulcer sites. Firmicutes, Proteobacteria, and Bacteroidetes were most abundant in the healthy group and in ulcer, nonulcer, and healed ulcer sites at the phylum level. Meanwhile, the number of Prevotella was significantly elevated in ulcer sites ( P < 0.05 ). Healthy people had more species of bacteria inhabiting their oral mucosa than did RAS patients, and patients with ulcers had the lowest abundance of bacterial species. We suggest that the number of Prevotella is associated with RAS.

A local dark tea – Liubao tea – extract exhibits remarkable performance in oral tissue regeneration, inflammation relief and oral microbiota reconstruction

“…75 Previous studies have shown that Proteobacteria exhibited a rising trend in the area of the mouth mucosa ulcer. 76 Also, it increased during the onset and progression of periodontitis, while the abundance of Firmicutes and Actinobacteria decreased. 77,78 Firmicutes are the primary beneficial bacteria in the saliva and buccal mucosa, 79 which is a huge bacterial phylum encompassing over 250 genera, including Lactobacillus, Faecalibacterium, Eubacterium, Roseburia and Anaerostipes.…”

Comparison of the main pathogenic microorganisms of various common oral diseases in children and adults

“…Some subpopulations of Prevotella, Neisseria, and Streptococcus, such as Prevotella melaninogenicus , Neisseria lactamica , Neisseria mucosa , Neisseria saprophaga , Neisseria subflava , Streptococcus viridans , S. mutans , and S. aureus , may be related to the occurrence of RAU 108 . The significant increase in the number of Prevotella at the ulcer site more effectively indicates that Prevotella is closely related to RAU 109 . For Helicobacter pylori , there is still no direct evidence to prove that it is related to the occurrence and development of RAU 110 .…”

“…108 The significant increase in the number of Prevotella at the ulcer site more effectively indicates that Prevotella is closely related to RAU. 109 For Helicobacter pylori , there is still no direct evidence to prove that it is related to the occurrence and development of RAU. 110 There is less research on viruses.…”

Anti-inflammatory and tissue repair effect of cinnamaldehyde and nano cinnamaldehyde on gingival fibroblasts and macrophages

“…One of the most prevalent conditions affecting the oral mucosa is recurrent aphthous stomatitis.Three kinds of the condition are distinguished clinically: minor, major, and herpetic form [ 1 ]. Minor aphthous lesions are less than 5 mm in size, circular, have a definite border, are painful, and heal without leaving scars in ten to fourteen days [ 2 ].…”