Detection and Characterization of Yersinia enterocolitica from Pigs and Cattle

Bonardi, S.; Paris, A.; Bacci, C.; D’Incau, Mario; Ferroni, Laura; Brindani, F.

doi:10.1007/s11259-007-0034-3

Cited by 5 publications

(4 citation statements)

References 7 publications

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“…Y. enterocolitica was detected on 3 (6.1%) of the 49 farms, with a prevalence of 1.6% of fecal samples from cattle, consistent with findings in previous studies. Prevalence can range from 0% as reported by Bailey et al (3) and Bucher et al (9), to 6.3 and 4.5% as reported in two British studies (39,42), and up to 14.3% (7). In the present study, Y. enterocolitica was detected in cattle but at a very low prevalence.…”

Section: Discussionsupporting

confidence: 58%

Occurrence of Zoonotic Clostridia and Yersinia in Healthy Cattle

Schmid¹,

Messelhäußer²,

Hörmansdorfer³

et al. 2013

Journal of Food Protection

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Zoonotic pathogens are a frequent cause of disease worldwide. This study was designed to determine the occurrence of Clostridium difficile, Clostridium botulinum, and Yersinia enterocolitica in cattle in southern Bavaria, Germany. The study population included 49 farms; 34 were dairy farms (30 also fattening beef cattle) and 15 were solely beef cattle farms. Fecal and dust samples were collected from summer 2011 to summer 2012 and analyzed using a combination of enrichment procedures and real-time PCR. For the detection of C. difficile, samples were screened for the presence of the tpi gene and toxin genes tcdA, tcdB, and cdtA. Samples also were screened for genes for C. botulinum toxins A through F and for the ail gene of Y. enterocolitica. Of 506 samples, C. difficile genes were found in 29 samples (5.7%): 25 samples from dairy farms and 4 samples from beef cattle farms. Toxin genes were identified in 17 samples, with toxigenic profiles of A(+)B(+)CDT(-), A(+)B(-)CDT(+), and A(+)B(+)CDT(+). C. botulinum toxin genes were not detected in fecal samples from cattle, but the gene for toxin B was detected in 1 (0.8%) of 125 dust samples. Y. enterocolitica genes were found in 6 (1.6%) of 382 fecal samples from three dairy farms and one beef cattle farm. This study revealed that C. difficile and Y. enterocolitica are rare on cattle farms in Bavaria, Germany. In contrast to results of previous studies, C. botulinum was not detected in fecal samples but was found very rarely in dust samples from the cattle environment.

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Section: Discussionsupporting

confidence: 58%

Occurrence of Zoonotic Clostridia and Yersinia in Healthy Cattle

Schmid¹,

Messelhäußer²,

Hörmansdorfer³

et al. 2013

Journal of Food Protection

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“…Only pork was found to be contaminated by pathogenic Y. enterocolitica 4/O:3 and 2/O:9, while all isolates from beef belonged to non-pathogenic biotype 1A. Pathogenic Y. enterocolitica bioserovars (3/O:5,27 and 3/O:9) were identified in cattle [32,33]. Since pathogenic Y. enterocolitica (2/O:5,27) was found in bulk milk at dairy farms, and improperly treated pasteurized milk, contaminated with Y. enterocolitica, was reported to be the source of yersiniosis outbreak, beef and cattle could be involved in the epidemiology of yersinosis [34,35].…”

Section: Discussionmentioning

confidence: 97%

Virulence Determinants and Genetic Diversity of Yersinia Species Isolated from Retail Meat

et al. 2021

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Yersinia enterocolitica is an important foodborne pathogen, and the determination of its virulence factors and genetic diversity within the food chain could help understand the epidemiology of yersiniosis. The aim of the present study was to detect the prevalence, and characterize the virulence determinants and genetic diversity, of Yersinia species isolated from meat. A total of 330 samples of retailed beef (n = 150) and pork (n = 180) in Latvia were investigated with culture and molecular methods. Whole genome sequencing (WGS) was applied for the detection of virulence and genetic diversity. The antimicrobial resistance of pathogenic Y. enterocolitica isolates was detected in accordance with EUCAST. Yersinia species were isolated from 24% (79/330) of meats, and the prevalence of Y. enterocolitica in pork (24%, 44/180) was significantly higher (p < 0.05) than in beef (13%, 19/150). Y. enterocolitica pathogenic bioserovars 2/O:9 and 4/O:3 were isolated from pork samples (3%, 6/180). Only resistance to ampicillin was confirmed in Y. enterocolitica 4/O:3 and 2/O:9 isolates, but not in other antimicrobials. Major virulence determinants, including ail, inv, virF, ystA and myfA, were confirmed with WGS in Y. enterocolitica 2/O:9 and 4/O:3. MLST typing revealed 15 STs (sequence types) of Y. enterocolitica with ST12 and ST18, which were associated with pathogenic bioserovars. For Y. enterocolitica 1A, Y. kristensenii, Y. intermedia and Y. frederiksenii, novel STs were registered (ST680-688). The presence of virulence genes and genetic characteristics of certain Y. enterocolitica STs confirm the common knowledge that pork could be an important source of pathogenic Yersinia.

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“…enterocolitica O:9 demonstrate cross reactivity of its smooth liposaccharide with that of Brucella , which masks the diagnosis of brucellosis caused by the latter bacterium [ 59 ]. Its indirect route of transmission is mainly oral ingestion of contaminated foods of animal and plant origins (e.g., pork, beef, lamb, unpasteurized milk, raw milk, vegetables, ice creams and seafoods) as well as contaminated water [ 60 , 61 ].…”

Section: Microbial Composition Of Cattle Manurementioning

confidence: 99%

An Overview of the Control of Bacterial Pathogens in Cattle Manure

Manyi-Loh

Mamphweli

Meyer

et al. 2016

IJERPH

207

106

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Cattle manure harbors microbial constituents that make it a potential source of pollution in the environment and infections in humans. Knowledge of, and microbial assessment of, manure is crucial in a bid to prevent public health and environmental hazards through the development of better management practices and policies that should govern manure handling. Physical, chemical and biological methods to reduce pathogen population in manure do exist, but are faced with challenges such as cost, odor pollution, green house gas emission, etc. Consequently, anaerobic digestion of animal manure is currently one of the most widely used treatment method that can help to salvage the above-mentioned adverse effects and in addition, produces biogas that can serve as an alternative/complementary source of energy. However, this method has to be monitored closely as it could be fraught with challenges during operation, caused by the inherent characteristics of the manure. In addition, to further reduce bacterial pathogens to a significant level, anaerobic digestion can be combined with other methods such as thermal, aerobic and physical methods. In this paper, we review the bacterial composition of cattle manure as well as methods engaged in the control of pathogenic microbes present in manure and recommendations that need to be respected and implemented in order to prevent microbial contamination of the environment, animals and humans.

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Detection and Characterization of Yersinia enterocolitica from Pigs and Cattle

Cited by 5 publications

References 7 publications

Occurrence of Zoonotic Clostridia and Yersinia in Healthy Cattle

Occurrence of Zoonotic Clostridia and Yersinia in Healthy Cattle

Virulence Determinants and Genetic Diversity of Yersinia Species Isolated from Retail Meat

An Overview of the Control of Bacterial Pathogens in Cattle Manure

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