Detection and differentiation of human papillomavirus genotypes HPV-6 and HPV-11 by FRET-based real-time PCR

Kocjan, Boštjan J.; Seme, Katja; Poljak, Mario

doi:10.1016/j.jviromet.2008.07.014

Cited by 37 publications

(21 citation statements)

References 33 publications

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“…The data about the disease extension, surgical procedures, HPV type causing RRP determined by RT-PCR [35], histopathological characteristics of the papillomas, and previous adjuvant treatment were obtained from the medical documentation.…”

Section: Methodsmentioning

confidence: 99%

Human papilloma virus vaccination in patients with an aggressive course of recurrent respiratory papillomatosis

Boltežar

Matičič

Šereg-Bahar

et al. 2014

Eur Arch Otorhinolaryngol

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In the case of an aggressive course of recurrent respiratory papillomatosis (RRP), adjuvant therapy can be used besides surgery. The aim of the study was to investigate the influence of vaccination with a quadrivalent vaccine against human papilloma viruses (HPV) types 6, 11, 16 and 18 on the course of RRP. Eleven subjects aged 13-46 years with a rapid growth of laryngeal papillomas were included in the study. They were vaccinated with three doses of the quadrivalent prophylactic HPV vaccine (Silgard(®), MSD) and followed up for 12-52 months. The intervals between the successive surgical procedures, the extension of the disease (Derkay score) at each surgery, and the number of surgical procedures per year before vaccination and after its completion were compared. The mean interval between the surgical procedures was 271.2 days before the vaccination and 537.4 days after it (p = 0.034). The mean number of surgeries per year was 2.16 before the vaccination and 0.93 after it (p = 0.022). The Derkay score did not change significantly after vaccination. Complete remission of the disease was observed in one patient, partial response to the vaccination was observed in seven patients and no response was observed in three patients. In conclusion, vaccination with the quadrivalent HPV vaccine can favorably influence the course of RRP in patients with the rapid growth of the papillomas. It significantly prolongs the intervals between the surgical procedures and reduces the number of procedures needed in the majority of patients. The present investigation can serve as a pilot study for further research. For a final conclusion a longer follow-up and studies on more patients are necessary.

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Section: Methodsmentioning

confidence: 99%

Human papilloma virus vaccination in patients with an aggressive course of recurrent respiratory papillomatosis

Boltežar

Matičič

Šereg-Bahar

et al. 2014

Eur Arch Otorhinolaryngol

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“…Thus, it has been used to quantify viral load and differentiate viral genotypes, such as European and American strains of porcine reproductive and respiratory syndrome virus (Egli et al, 2001), vaccine and field isolates of avian reovirus (Ke et al, 2006), human papillomavirus genotypes HPV-6 and HPV-11 (Kocjan et al, 2008), tomato yellow leaf curl virus and tomato yellow leaf curl Sardinia virus (Papayiannis et al, 2010), and lyssavirus genotypes 1, 5, and 6 (Wakeley et al, 2005). Current studies have reported that the S gene of the current Chinese PEDV field strain CH/FJND-3 has the lowest identity with that of PEDV strain CV777 and has a 15-bp insertion and a 6-bp deletion compared with CV777 .…”

Section: Discussionmentioning

confidence: 99%

Development of a multiplex TaqMan probe-based real-time PCR for discrimination of variant and classical porcine epidemic diarrhea virus

Zhao

Bai

Jiang

et al. 2014

Journal of Virological Methods

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“…To date, RT-PCR is the only technique capable of identifying closely related HPV strains. The group of Poljack has demonstrated a beautiful use of fluorescence resonance energy transfer (FRET) coupled with RT-PCR to achieve the identification of 19 different HPV strains that are important because of their oncogenic risk [45]. They successfully identified several HPV strains mixed in the same sample, with a presence as low as 25-43 DNA strands per strain.…”

Section: Optical Biosensorsmentioning

confidence: 99%

Trends in Biosensors for HPV: Identification and Diagnosis

et al. 2015

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The conventional methodologies used for the detection of human papillomavirus (HPV) present actually robust and reproducible advantages. However, at the same time, they involve complex protocols that sometimes are difficult to popularize. Over the first half of XX century, the adequate treatment of complex and delicate processes from a simple instrumental base seemed a fundamental and intrinsic contradiction. However, interdisciplinary trends have allowed the manipulation of tissues, proteins, and nucleic acids through innovative increasingly smaller devices. The proper diagnosis of HPV has seen great advances since biosensor researchers are employing its virus strains as models to study the interactions between the biorecognition element and the transducer. Additionally, all recent improvements and trends that material sciences, biotechnology, and data processing scientists excel for biosensors can be applied for the HPV detection platforms. In this review, we highlight the recent trends on materials, nanomaterials, and transducers for the specific detection and differentiation of HPV strains. The most influential methods for the detection and identification of these papillomaviruses include optical, electrochemical, and piezoelectric transducers; we will visit their sensibility and advantages. Additionally, we highlight the factors that contributed to the increasing importance of these biodevices as potential substitutes to conventional diagnostic methods.

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Detection and differentiation of human papillomavirus genotypes HPV-6 and HPV-11 by FRET-based real-time PCR

Cited by 37 publications

References 33 publications

Human papilloma virus vaccination in patients with an aggressive course of recurrent respiratory papillomatosis

Human papilloma virus vaccination in patients with an aggressive course of recurrent respiratory papillomatosis

Development of a multiplex TaqMan probe-based real-time PCR for discrimination of variant and classical porcine epidemic diarrhea virus

Trends in Biosensors for HPV: Identification and Diagnosis

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