Detection and discrimination of Mycobacterium tuberculosis complex

Issa, Rahizan; Hassan, Nurul Akma Mohd; Abdul, Hatijah; Hashim, Siti Hasmah; Seradja, Valentinus H.; Sani, Athirah Abdul

doi:10.1016/j.diagmicrobio.2011.09.021

Cited by 5 publications

(3 citation statements)

References 18 publications

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“…S1b). The amplification efficiency of the assay showed reliable and better quantification of the target gene compared with the previous study (Issa et al , 2012).…”

Section: Resultsmentioning

confidence: 58%

High resolution melting analysis for the differentiation of Mycobacterium species

Issa

Abdul

Hashim

et al. 2014

Journal of Medical Microbiology

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A quantitative real-time PCR (qPCR) followed by high resolution melting (HRM) analysis was developed for the differentiation of Mycobacterium species. Rapid differentiation of Mycobacterium species is necessary for the effective diagnosis and management of tuberculosis. In this study, the 16S rRNA gene was tested as the target since this has been identified as a suitable target for the identification of mycobacteria species. During the temperature gradient and primer optimization process, the melting peak (Tm) analysis was determined at a concentration of 50 ng DNA template and 0.3, 0.4 and 0.5 mM primer. The qPCR assay for the detection of other mycobacterial species was done at the Tm and primer concentration of 62 6C and 0.4 mM, respectively. The HRM analysis generated cluster patterns that were specific and sensitive to distinguished small sequence differences of the Mycobacterium species. This study suggests that the 16S rRNA-based real-time PCR followed by HRM analysis produced unique cluster patterns for species of Mycobacterium and could differentiate the closely related mycobacteria species.

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“…S1b). The amplification efficiency of the assay showed reliable and better quantification of the target gene compared with the previous study (Issa et al , 2012).…”

Section: Resultsmentioning

confidence: 58%

High resolution melting analysis for the differentiation of Mycobacterium species

Issa

Abdul

Hashim

et al. 2014

Journal of Medical Microbiology

Self Cite

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“…Mycobacterium tuberculosis complex group (MTBC) usually enters the body through hangnail wounds, small wounds, impetigo, or furuncles (Hill and Sanders, 2017;Bespiatykh et al, 2021;Kanabalan et al, 2021). Its pathogenesis is similar to that of some other epidemic diseases (Issa et al, 2012). Over 2-4 weeks, Mycobacterium enters the body and slowly develops into a tuberculous chancre.…”

Section: Introductionmentioning

confidence: 65%

Application of metagenomic next-generation sequencing in cutaneous tuberculosis

Kang

Kong

et al. 2022

Front. Cell. Infect. Microbiol.

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Tuberculous infection in a skin wound is a rare but well-known condition. This study describes a child infected with tuberculosis after being wounded. Because of swelling and pain in his wrist tissue, he was admitted to the Affiliated Hospital of Jining Medical University of Shandong Province on 16 October 2021. His medical history only included a wound. He was discharged after debridement. The laboratory data were normal. Two months after surgery, his wound was still swollen and painful. Secretions from the wound were sent for metagenomic next-generation sequencing (mNGS), which revealed three reads related to the Mycobacterium tuberculosis complex group (MTBC). A diagnosis of cutaneous tuberculosis (TB) was made. The wound disappeared after anti-TB drugs were administered. This case demonstrates that, while TB presenting as a severe cutaneous wound is rare, it should be considered in the clinical diagnosis. Clinicians should also pay attention to extrapulmonary infection with MTBC in patients, particularly in some long-suffering patients, and identify the specific pathogen as soon as possible. mNGS could help to identify pathogens and facilitate early treatment, thereby improving the prognosis.

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“…According to the World Health Organization (WHO), 8.5-9.2 million new TB cases were estimated to have occurred in 2010 [1], and 1.2–1.5 million deaths were caused by species of the Mycobacterium tuberculosis complex (MTBC) that includes M. tuberculosis , M. bovis, M. bovis BCG (vaccine strain), M. africanum, M. microti, M. canettii, and M. pinnipedii, which produces TB in humans and some animal hosts [2,3]. Part of the infected population will develop active TB, whereas the majority of cases (approximately 90%) progress to a non-infectious disease or latent TB, where mycobacteria survive in a dormant state inside immune cells [4].…”

Section: Introductionmentioning

confidence: 99%

In silico identification and characterization of the ion transport specificity for P-type ATPases in the Mycobacterium tuberculosis complex

et al. 2012

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BackgroundP-type ATPases hydrolyze ATP and release energy that is used in the transport of ions against electrochemical gradients across plasma membranes, making these proteins essential for cell viability. Currently, the distribution and function of these ion transporters in mycobacteria are poorly understood.ResultsIn this study, probabilistic profiles were constructed based on hidden Markov models to identify and classify P-type ATPases in the Mycobacterium tuberculosis complex (MTBC) according to the type of ion transported across the plasma membrane. Topology, hydrophobicity profiles and conserved motifs were analyzed to correlate amino acid sequences of P-type ATPases and ion transport specificity. Twelve candidate P-type ATPases annotated in the M. tuberculosis H37Rv proteome were identified in all members of the MTBC, and probabilistic profiles classified them into one of the following three groups: heavy metal cation transporters, alkaline and alkaline earth metal cation transporters, and the beta subunit of a prokaryotic potassium pump. Interestingly, counterparts of the non-catalytic beta subunits of Hydrogen/Potassium and Sodium/Potassium P-type ATPases were not found.ConclusionsThe high content of heavy metal transporters found in the MTBC suggests that they could play an important role in the ability of M. tuberculosis to survive inside macrophages, where tubercle bacilli face high levels of toxic metals. Finally, the results obtained in this work provide a starting point for experimental studies that may elucidate the ion specificity of the MTBC P-type ATPases and their role in mycobacterial infections.

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Detection and discrimination of Mycobacterium tuberculosis complex

Cited by 5 publications

References 18 publications

High resolution melting analysis for the differentiation of Mycobacterium species

High resolution melting analysis for the differentiation of Mycobacterium species

Application of metagenomic next-generation sequencing in cutaneous tuberculosis

In silico identification and characterization of the ion transport specificity for P-type ATPases in the Mycobacterium tuberculosis complex

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