Detection and enumeration of airborne biocontaminants

Stetzenbach, Linda D.; Buttner, Mark P.; Cruz, Patricia

doi:10.1016/j.copbio.2004.04.009

Cited by 219 publications

(149 citation statements)

References 28 publications

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“…Quantitative PCR Because the quantity of an etiologic agent in aerosols is also important when assessing health risks to humans and the environment, there is a need for quantitative PCR methodology (Stetzenbach et al 2004). Real-time PCR (RT-PCR) is a quantitative PCR method that employs fluorescent dyes or probes to quantify the number of copies of a target DNA sequence in a sample.…”

Section: Microarraymentioning

confidence: 99%

Qualitative and quantitative methodologies for determination of airborne microorganisms at concentrated animal-feeding operations

Dungan

Leytem

2009

World J Microbiol Biotechnol

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The generation of airborne microorganisms from concentrated animal-feeding operations (CAFOs) is a concern from a human and animal health perspective. To better understand the airborne microorganisms found in these environments, a number of collection and analytical techniques have been utilized and will be discussed in this review. The most commonly used bioaerosol collection method is the liquid impingement format, which is suitable with a number of culture-based and non-culture molecularbased approaches, such as polymerase chain reaction. However, the vast majority of airborne microorganism studies conducted at CAFOs utilize culture-based analyses. Because of the limitations often associated with culturebased analyses, we focused our discussion on the application of molecular-based techniques to identify and/or quantify microorganisms, as they have promising application in bioaerosol research. The ability to rapidly characterize airborne microorganisms will help to ensure protection of public and environmental health.

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Section: Microarraymentioning

confidence: 99%

Qualitative and quantitative methodologies for determination of airborne microorganisms at concentrated animal-feeding operations

Dungan

Leytem

2009

World J Microbiol Biotechnol

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“…Along with living or dead fungi and bacteria (both pathogenic and non-pathogenic), bioaerosol may also consist of peptidoglycans, endotoxins, mycotoxins, pollens etc. (Douwes et al, 2003;Stetzenbach et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of Size-Segregated Bioaerosols at Different Sites in Delhi

Lal

Ghosh

Srivastava

et al. 2017

Aerosol Air Qual. Res.

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Ambient levels of culturable bioaerosol were measured at four different sites of Delhi, India in six size ranges (> 7.0 µm, 7.0-4.7 µm, 4.7-3.3 µm, 3.3-2.1 µm, 2.1-1.1 µm, < 1.1 µm). The study also accounted the seasonal variation (monsoon, post monsoon, winter and pre-monsoon) of the air microbes. The sampling was carried out for three different fractions of bioaerosols viz. fungi, gram positive and negative bacteria during August 2010 to April 2011 using a six-stage viable cascade impactor sampler. Unlike gram positive and negative bacteria, the concentration of fungal bioaerosol found in different stages at each site seems to follow a typical pattern in all four season. The typical pattern of concentration depicts that majority of the fungal species found in the diameter range of 3.3-2.1 µm, which coincides with the penetration range in the secondary bronchi of the lungs in the human body. This reveals that majority of the immunotoxic and allergic fungi found at this stage are mostly prone to affect the secondary bronchi in human lungs when inhaled. At all four sites maximum fungal concentration (1740.5-3224.7 CFU m -3 ), gram-positive bacterial concentration (2790.6-9428.3 CFU m -3 ) and gram-negative bacterial concentration (1990.3-7609 CFU m -3 ) were found in post monsoon season. In the majority of the sites, minimum concentrations were found in monsoon period which probably may be due to rain wash during the sampling. For all the three bioaerosol fractions no particular relationship pattern was found to exist between their respective concentrations with temperature and relative humidity (RH). However, higher range of variation was observed at higher concentration levels and lower range of variation at low concentration levels for all the three bioaerosol fractions. Most of the fungal bioaerosol identified such as Penicillium sp., Alternaria sp. and Aspergillus sp. are associated with immunotoxic and allergic diseases.

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“…The last measurement point (location 5) considerably differed from other ones in this respect. Microorganisms, including E. coli, can be transferred over long distances (Cox & Wathes, 1995, Radon, 2007, Ritz et al, 2006, Stetzenbach et al, 2004. It is important that such objects were specially secured and fenced with screens preventing the entry of contaminated air to the surrounding areas.…”

Section: Resultsmentioning

confidence: 99%

Microbiological Air Rating in a Variety of Objects During Treatment of the Post-Slaughter Poultry Wastes Part I. Escherichia Coli

Mahdi-Oraibi¹,

Cybulska²

2016

J. Ecol. Eng.

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The presence of Escherichia coli in the air of facilities involved in management and composting of post-slaughter poultry wastes in selected plants of West Western Pomerania region was studied. Measurements were made on four dates in a variety of weather conditions during the year. The study was conducted at 5 objects that differ in the type of waste and the degree of preparation for composting. These were: chemical treatment and preliminary processing plant, liquid wastes reservoir, platform for preparation of materials for composting, storage of biological sediments, and composting facility. Measurement of bacteria count was carried out in accordance with the applicable procedures on selective chromogenic TBX medium. The assays revealed the presence of E. coli at all test objects, but not always on all measurement dates. It has been shown that the presence of E. coli was from 20 to 3047 CFU•m -3 of air, although the largest quantities were most frequently detected in the air of the building for post-slaughter waste pre-treatment in chemical treatment plant.

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Detection and enumeration of airborne biocontaminants

Cited by 219 publications

References 28 publications

Qualitative and quantitative methodologies for determination of airborne microorganisms at concentrated animal-feeding operations

Qualitative and quantitative methodologies for determination of airborne microorganisms at concentrated animal-feeding operations

Identification and Characterization of Size-Segregated Bioaerosols at Different Sites in Delhi

Microbiological Air Rating in a Variety of Objects During Treatment of the Post-Slaughter Poultry Wastes Part I. Escherichia Coli

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