Detection and Frequency of Lily Viruses in Argentina

Abstract

In a survey of lily growing fields in various regions of Argentina, three viruses, Lily symptomless virus (LSV), Lily mottle virus (LMoV), and Cucumber mosaic virus (CMV), were found in Longiflorum, Asiatic, Oriental, Longiflorum × Asiatic (LA), and Oriental × Trumpet (OT) hybrids. The areas surveyed were between latitude 26° 56′ S and 43° 03′ S, and longitude 65° 21′ W and 71° 29′ W. Virus detection was performed by double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) using polyclonal antiserum. In infected samples, viruses detected in decreasing order were LSV (60.5%), LMoV (51.0%), and CMV (28.7%) present in single or mixed infections. Virus infection varied among tested hybrids from 36.0% (Oriental Montecristo) to 94.7% (Lilium longiflorum Avita) in 2006 and from 38.9% (OT Yelloween) to 82.1% (LO Triumphator) in 2007, with an overall incidence of 64.1 and 70.7% in 2006 and 2007, respectively. A variation in virus incidence among localities was also observed. The highest virus incidence (89.6 and 87.6% in 2006 and 2007, respectively) was observed in Bahía Blanca (38° 44′ S, 62° 16′ W). The lowest virus incidences, detected in Trevellin (43° 03′ S, 71° 29′ W) and in Malargüe (35° 28′ S, 69° 35′ W), were 47.4 and 48.6% in 2006 and 2007, respectively. Moreover, a different distribution of each virus was observed between localities. The high occurrence of viruses infecting lily crops in Argentina could be due to both the use of infected bulbs for propagation and the lack of preventive virus vector control measures.

Occurrence and molecular analysis of quarantine virus in lily cultivation areas in Brazil

“…The results show that LSV single infections were less frequent (21%) than mixed infections with LMoV and CMV. The simultaneous infection by these viruses in lilies was also reported in Argentina, India, and Japan (Niimi et al, 2003;Sharma et al, 2005;Chinestra et al, 2010).…”

“…Only CMV could be transmitted from lily to experimental hosts through mechanical inoculation, which was confirmed by Elisa and RT-PCR. Because CMV infects lily and is widespread in the commercial cultivation of this plant (Chinestra et al, 2010;Nesi et al, 2011), assays were previously carried out to detect CMV in single, dual and triple infections with LSV and LMoV in cultivated lilies. The presence of mixed infection by these viruses intensifies symptoms (Zheng et al, 2003;Sharma et al, 2005) and increases economic losses (Zheng et al, 2003); therefore, CMV should be considered when diagnosing infection on imported bulbs.…”

“…In addition to sharing the same ancestor, both viruses display a narrow natural host range, with LSV infecting Lilium spp. (Niimi et al, 2003;Zheng et al, 2003;Chinestra et al, 2010), Tulipa sp. (Nesi et al, 2011), Alstroemeria sp.…”

“…Most studies on CMV, LMoV, and LSV in lilies addressed their global spread (Zheng et al, 2003;Sharma et al, 2005;Nesi et al, 2011;Chinestra et al, 2010), detection (Niimi et al, 2003;Nesi et al, 2011), and phylogeny (Yamaji et al, 2001;Masuta et al, 2002;Singh et al, 2008). Few reports on recombinations among LSV isolates (Singh et al, 2008) and taxonomic definitions concerning LMoV or TBV in lilies (Derks et al, 1994;Yamaji et al, 2001) are available in the literature, but no recombination event has been described for this virus.…”

Elimination of Lily Symptomless Virus by In Vitro Scaling and Reinfection Rates under Various Culture Conditions in Korean Native Lilies

“…바이러스 감염 정도는 항체를 이용한 direct tissue blotting immunobinding assay(DTBIA)와 enzyme-linked immuno-sorbent assay(ELISA)에 의해 진단할 수 있으며 , 조기 발견과 정확한 진단에는 reverse transcription polymerase chain reaction(RT-PCR) 분석을 이용할 수 있다 (Joung et al, 1996;Kim et al, 2001). 그러나 대부분의 연 구가 국내 재배면적이 월등한 수입 품종에 집중되어 있고 (Chinestra et al, 2010;Seo et al, 1999;Woo et al, 2005) (Allen and Anderson, 1980;Blom-Barnhoorn et al, 1986;Chang and Kim, 1996;Horst, 1988;Kassanis, 1957;Lawson, 1990). Table 2.…”