Elimination of Lily Symptomless Virus by In Vitro Scaling and Reinfection Rates under Various Culture Conditions in Korean Native Lilies

Kim, Min Hui; Park, In Sook; Park, Kyeung Il; Oh, Wook; Kim, Kiu Weon

doi:10.7235/hort.2015.15091

Cited by 3 publications

(4 citation statements)

References 17 publications

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“…This is due to the stability, high productivity, and easy protein purification [28,82]. Consequently, the ID-ELISA method and IC-RT-PCR assays have been developed for efficient lily virus detection [104]. These methods were recommended for further control, surveys, and also to prevent lily infection by most of the viruses that occur in Lanzhou/Gansu Province and NingXia, the most important lily production areas in China [21,98].…”

Section: Generation and Purification Of Recombinant Coat Proteinsmentioning

confidence: 99%

Advancements in Lily Viruses Management: Challenges and Solutions in Elimination and Detection

Gong

Dusengemungu

et al. 2023

Horticulturae

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Lilies are important crops that are commonly used as cut flowers (Lilium spp.) and edible bulb crops (Lilium davidii var. unicolor). However, virus infections can significantly impact the quantity and quality of lily production. Various methods have been developed to eliminate viruses in lilies, including in vitro culture and virus detection techniques. Meristem culture is the most effective method, which can be combined with other techniques such as thermotherapy and chemotherapy. Nonetheless, virus elimination is affected by several factors, including cultivar, explants used, virus type, and duration of treatments. Efficient diagnostic methods, such as serological and molecular techniques, have been developed to detect viral infections in lilies, including enzyme-linked immunosorbent assay (ELISA) and real-time reverse transcriptase polymerase chain reaction (real-time RT-PCR). However, cross-contamination and multiple-virus contamination can lead to unreliable results, and more sophisticated protocols and systems have been developed to address these issues. The objective of this review is to provide a comprehensive overview of the development of lily virus eradication, detection strategies, challenges, and solutions associated with these procedures, and how more sophisticated approaches such as multiplex RT-PCR, indirect ELISA (ID-ELISA), immunocapture RT-PCR (IC-RT-PCR), and immunochromatographic test strips (ICSs) can alleviate some of these setbacks.

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Section: Generation and Purification Of Recombinant Coat Proteinsmentioning

confidence: 99%

Advancements in Lily Viruses Management: Challenges and Solutions in Elimination and Detection

Gong

Dusengemungu

et al. 2023

Horticulturae

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“…Other lily-infecting viruses have been reported to occur only in some regions. In Korea, the incidence of LSV has reached an infection rate of 45.0% ( Kim et al, 2015 ). In China, LSV, CMV, and LMoV are also widely distributed in major lily-production regions including Gansu, Ningxia, Qinghai, Jiangsu, and Yunnan provinces.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and phylogenetic characteristics of viruses in lily plants in Beijing

et al. 2023

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Lily (Lilium) is an important bulbous perennial herb that is frequently infected by one or more viruses. To investigate the diversity of lily viruses, lilies with virus-like symptoms in Beijing were collected to perform small RNA deep sequencing. Then, the 12 complete and six nearly full-length viral genomes, including six known viruses and two novel viruses were determined. Based on sequence and phylogenetic analyses, two novel viruses were considered to be members of the genera Alphaendornavirus (Endornaviridae) and Polerovirus (Solemoviridae). These two novel viruses were provisionally named lily-associated alphaendornavirus 1 (LaEV-1) and lily-associated polerovirus 1 (LaPV-1). Based on sequence, phylogenetic and recombination analyses, strawberry latent ringspot virus (SLRSV) in the genus Stralarivirus (Secoviridae) was identified for the first time in China, and shown to exhibit the highest nucleotide (nt) diversity among the available full-length SLRSV genome sequences, with the highest identities of 79.5% for RNA1 and 80.9% for RNA2. Interestingly, the protease cofactor region in RNA1 was 752 aa in length, whereas those of the other 27 characterized isolates ranged from 700–719 aa in length. The genome sequences of lily virus A (Potyvirus), lily virus X (Potexvirus), and plantago asiatica mosaic virus (Potexvirus) exhibited varying degrees of sequence diversity at the nucleotide level compared with their corresponding characterized isolates. In addition, plantago asiatica mosaic virus (PlAMV) tended to cluster on a host species-basis. One identified lily mottle virus (Potyvirus) isolate was detected as a recombinant, and which clustered in a different group with four other isolates. Seven identified lily symptomless virus (Carlavirus) isolates, including one recombinant, were clustered into three clades. Our results revealed the genetic diversity of lily-infecting viruses, and sequence insertion, host species and recombination are factors that likely contribute to this diversity. Collectively, our results provide useful information regarding the control of viral disease in lily.

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“…Lilies native to Korea are part of the original species of Asiatic hybrids. Owing to their unique ornamental value, they can be used as cut flowers or for differentiation and gardening (Kim et al, 2015;RDA, 2021). Earlier, native lilies were propagated by seed propagation and scaling.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the systematic production and distribution of high-quality bulbs is absent in Korea (Kim et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Effects of the Propagation Method and Planting Season of Bulblets on Shoot Emergence and Bulb Enlargement in Korean Native Lilies in Sinomartagon Section

Kim,

Kim

et al. 2022

HST

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This study investigated the effects of the planting season on shoot emergence and the enlargement of bulblets via three propagation methods in Korean native lilies belonging to the Sinomartagon section. One-year-old bulblets of Lilium amabile, L. cernuum, L. lancifolium, and L. leichtlinii var. maximowiczii were obtained through tissue culture, scaling, and seed propagation steps. They were divided into two groups based on the species and propagation method and planted in an open field either immediately in autumn or in spring after wet cold storage. Overall, spring planting increased the shoot emergence rate, delayed the shoot emergence date, and increased bulb fresh weight (BFW) and the bulb enlargement rate (BER) at harvest compared to autumn planting. In terms of the propagation method used to obtain bulblets, BERs were higher in the following order: seed propagation > scaling > tissue culture. Bulblet fresh weights just before planting and BFW at harvest showed an almost proportional relationship regardless of the species, whereas the slope decreased as the bulblet fresh weight exceeded 1.5 g. In terms of species, genetically close L. amabile and L. lancifolium had high BERs, and L. cernuum had a relatively low BER. In conclusion, with regard to bulb production of four Korean native species belonging to the Sinomartagon section, regardless of the propagation method used to obtain bulblets, wet cold storage without planting the bulblets in autumn and subsequently planting them in an open field or greenhouse in the spring for intensive management would be the key to bulb production.

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Elimination of Lily Symptomless Virus by In Vitro Scaling and Reinfection Rates under Various Culture Conditions in Korean Native Lilies

Cited by 3 publications

References 17 publications

Advancements in Lily Viruses Management: Challenges and Solutions in Elimination and Detection

Advancements in Lily Viruses Management: Challenges and Solutions in Elimination and Detection

Genetic diversity and phylogenetic characteristics of viruses in lily plants in Beijing

Effects of the Propagation Method and Planting Season of Bulblets on Shoot Emergence and Bulb Enlargement in Korean Native Lilies in Sinomartagon Section

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