Detection and Genotyping of Coxiella burnetii and Coxiella-Like Bacteria in Horses in South Korea

Seo, Min-Goo; Lee, Seung-Hun; VanBik, Dorene; Ouh, In-Ohk; Yun, Sung Ho; Choi, Eunsang; Park, Yong Soo; Lee, Sang-Eun; Kim, Jong Wan; Cho, Gil‐Jae; Kwon, Oh-Deog; Kwak, Dongmi

doi:10.1371/journal.pone.0156710

Cited by 45 publications

(59 citation statements)

References 26 publications

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“…We previously investigated equine infections in Korea: the seroprevalence of C . burnetii was 1.3% (11/816) and the presence rate of CLE was 0.7% (6/816) [12]. Therefore, another aim of this study was to assess the differences between the C .…”

Section: Discussionmentioning

confidence: 99%

“…Over the last few decades, climate changes due to global warming have transformed the Korean climate from temperate to subtropical [12]. This may also explain the spread of Coxiella in vector ticks in Korea.…”

Section: Discussionmentioning

confidence: 99%

“…Most Korean areas are mountainous, and the warm summer season provides suitable conditions for the growth of ticks. From spring to autumn, ticks inhabit the entire country, and their periods of activity are extended [12]. Therefore, the seasonal occurrence frequency of ticks is variable, and the frequency of tick-borne diseases can vary as well.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, in Korea, C . burnetii and CLE were detected in horses [12]. Therefore, the aim of this study was to use nested PCR (nPCR) and genotyping to investigate the occurrence of C .…”

Section: Introductionmentioning

confidence: 99%

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Molecular Detection and Genotyping of Coxiella-Like Endosymbionts in Ticks that Infest Horses in South Korea

et al. 2016

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Members of the genus Coxiella can be transmitted from ticks to humans during contact with animals; Coxiella may thus spread from the infected horses or ticks to humans. In this study, the presence of Coxiella burnetii and Coxiella-like endosymbionts (CLE) in ticks found on infested horses was determined using PCR and genotyping. A total of 213 ticks were randomly collected from 51 horses (4–5 ticks per horse) raised on Jeju Island, Korea, between 2009 and 2013. All ticks were morphologically identified as adult Haemaphysalis longicornis, a predominant tick species widespread in Korea. Based on the results of nested PCR and 16S rRNA sequencing, CLE were detected in 121 (52.4%, 95% CI: 45.9–58.8) ticks. CLE 16S rRNA sequences from 9 randomly selected ticks were 100% identical. Phylogenetic analysis showed that these 9 sequences were highly similar (97.9–100%) to the sequences of clade B species, like the CLE previously described to be found in Haemaphysalis spp. This study showed that CLE are prevalent in ticks that infest horses reared on Jeju Island, and this is, to the best of our knowledge, the first study to describe CLE occurrence in ticks infesting animals reared in Korea. Because of the high prevalence of CLE in ticks found on horses, CLE transmission from ticks to other animals and humans remains a possibility. This warrants a detailed study of other hosts and regions. Considering the zoonotic potential of Coxiella, further strategic surveillance of Coxiella transmission is necessary.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Recently, in Korea, C . burnetii and CLE were detected in horses [12]. Therefore, the aim of this study was to use nested PCR (nPCR) and genotyping to investigate the occurrence of C .…”

Section: Introductionmentioning

confidence: 99%

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Molecular Detection and Genotyping of Coxiella-Like Endosymbionts in Ticks that Infest Horses in South Korea

et al. 2016

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“…Other Coxiella species have been reported as tick endosymbionts, that are transovarially inherited, and lack virulent characteristics Raele et al, 2015;Seo et al, 2016) and have also been reported in mammals (Angelakis et al, 2016;Seo et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Molecular investigation into the presence of a Coxiella sp. in Rhipicephalus sanguineus ticks in Australia

Oskam

Gofton

Greay

et al. 2017

Veterinary Microbiology

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Molecular investigation into the presence of a Coxiella sp.in Rhipicephalus sanguineus ticks in Australia.Veterinary Microbiology http://dx.doi.org/10. 1016/j.vetmic.2017.01.021 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. turanicus ticks overseas. This study illustrates the value of using genus specific PCR assays to detect previously unreported bacterial species. Furthermore, the presence of an additional Coxiella sp. in Australia requires further investigation into its potential for contributing to serological crossreactions during Q fever testing.

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Characterization of microbiota diversity of field‐collected Haemaphysalis longicornis (Acari: Ixodidae) with regard to sex and blood meals

Zhang

Huang

et al. 2018

J Basic Microbiol

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Haemaphysalis longicornis is a prominent tick species in China, and the major vector of an emerging tick‐borne disease: severe fever with thrombocytopenia syndrome (SFTS). Microbiome diversity of ticks is influenced by several factors. In this study, we investigated microbiome diversity in field‐collected female and male H. longicornis ticks and compared the microbial composition of fed and unfed ticks and of those feeding on different hosts using barcode sequencing of V3–V4 region of 16S RNA gene. Regardless of sex, host, and feeding status; the highest abundance among all samples was found for the genus Coxiella. The relative numbers of Coxiella sequences decreased with the length of the blood feeding, whereas the numbers of Staphylococcus and Corynebacterium increased gradually. The dominance of Coxiella across all samples indicates that it is an obligate symbiont of H. longicornis. Overall, higher microbiome richness was detected in male ticks than in female ticks. Fed ticks showed a more diverse microbe composition than unfed ticks, and ticks fed on goats exhibited the highest diversity. These findings of this study can serve as a basis for future studies of microbiota biology and interactions between the microbes and pathogens of H. longicornis.

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Detection and Genotyping of Coxiella burnetii and Coxiella-Like Bacteria in Horses in South Korea

Cited by 45 publications

References 26 publications

Molecular Detection and Genotyping of Coxiella-Like Endosymbionts in Ticks that Infest Horses in South Korea

Molecular Detection and Genotyping of Coxiella-Like Endosymbionts in Ticks that Infest Horses in South Korea

Molecular investigation into the presence of a Coxiella sp. in Rhipicephalus sanguineus ticks in Australia

Characterization of microbiota diversity of field‐collected Haemaphysalis longicornis (Acari: Ixodidae) with regard to sex and blood meals

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