Detection and genotyping of HPV in urine samples from Chilean women attending primary health care centers

Abstract

Cervical cancer is the second most common malignant neoplasm in women worldwide representing approximately 10% of all types of cancers. Triage of women through cervical cytology has been an important strategy for the surveillance and control of new cases of cervical cancer. However, in many regions around the world cervical cytology has a low coverage compared to developed countries. The molecular detection of HPV is the most effective method to increase the screening sensitivity of women at risk of developing cervical cancer. There are very few studies about the efficacy of urine testing for detection of HPV in women followed up in primary health care centers. Consequently, the efficacy of using urine HPV screening in these populations has not been addressed yet. Here, we compared the detection of HPV in simultaneous urine and cervical samples of women followed up in primary health care centers. Urine and cervical samples were analyzed in 543 women attending at primary health care centers. HPV was detected by real time PCR, and HPV typing performed by PCR-RLB. A general HPV concordance of 86.2% (κ = 0.72) was determined between urine and cervical samples. The concordance for HPV-16 and 18 was almost perfect (κ = 0.82) and strong (κ = 0.77), respectively. The sensitivity and specificity for all HPV genotypes in urine using cervical samples as reference were 82.1 and 93.7%, respectively. The results showed that urine is a good alternative as clinical sample for HPV screening in women attending primary health care centers. Therefore, urine should be used as an alternative sample for increasing triage coverage either in refractory women participating in Pap surveillance programs or when cervical samples are not available.

The preference of women living with HIV for the HPV self-sampling of urine at a rural HIV clinic in Uganda

“… 13 , 14 However, new literature suggests that urine sampling is potentially a more favourable and reliable method than HPV self-sampling using a vaginal swab, as it is less invasive and thus may increase CC screening coverage even more. 15 , 16 , 17 , 18 , 19 The urine HPV self-sampling approach works well within the clinic, with a good storage facility of −21 °C to maintain the viability and quality of the DNA sample. 15 , 16 , 17 , 18 , 19 However, no study has been done in Uganda to assess the preference for HPV self-sampling using urine in a rural setting.…”

Diagnostic Test Accuracy of First-Void Urine Human Papillomaviruses for Presence Cervical HPV in Women: Systematic Review and Meta-Analysis

“…Identifying and selecting studies is summarized in Figure 1. Of the 5054 potential records, 15 studies (3675 women recruited, 3412 women analysed) were included in the meta-analysis [3,16,17,[31][32][33][34][35][36][37][38][39][40][41][42].…”

“…Cervical cancer presents the fourth-most cause of cancer deaths in women worldwide [2]. HPV is detected in almost all cervical cancer biopsies with more than 90% presence in high-grade squamous intraepithelial lesions (HSIL) [3]. More than 200 genotypes of HPV have been identified to date [4].…”

“…Variables were retained in the regression model if p < 0.05 2. Relative diagnostic odds ratio 3. Constant coefficient 4.…”

The simultaneous detection of the squamous cell carcinoma antigen and cancer antigen 125 in the cervical cancer serum using nano-Ag polydopamine nanospheres in an SERS-based lateral flow immunoassay

“…1 Also, developing countries bear the major portion of the disease burden. 2 The current detection methods for the initial assessment of cervical cancer include the thinprep cytology test (TCT) and human papillomavirus (HPV) test and have low specicity and sensitivity. Additionally, colposcopy and cervical biopsy are the invasive tests, which make them difficult for patients to accept.…”