Detection and investigation of a protein factor concerned with the stimulation of disulfide reductase activity by cyclic AMP and other effectors

Kulinskii, V. I.; Kolesnichenko, L. S.

doi:10.1007/bf00802989

Cited by 5 publications

(6 citation statements)

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“…The glutathione redox system is one of the most important antioxidant systems in the body [3,6]. However, we found no publications concerning activity of this system in the skin of healthy humans and patients with dermatoses.…”

Section: Abstract: Skin; Dermis; Epidermis; Estradiol; Lipid Peroxidmentioning

confidence: 99%

Effects of sex steroid hormones on lipid peroxidation and glutathione antioxidant system in rat skin

1999

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Effects of estradiol and testosterone on the intensity of lipid peroxidation and contents of glutathione redox system components in the dermis and epidermis of rat skin were studied. Only estradiol induced considerable dose-dependent and tissue-specific biphasic antioxidant effects on the skin. Key Words: skin; dermis; epidermis; estradiol; lipid peroxidation; chemiluminescence; glutathioneLipid peroxidation (LPO) typical of all tissues and regulating cell proliferation, lipid-protein interactions, and membrane permeability [ 1] also affects cell membranes and their functions. The intensity of LPO is regulated by systems of free radical generation and antioxidant protection.LPO processes in the blood are activated in some skin diseases [4]. The pathogenesis and severity of dermatoses depend on the sex of a patient [8], which can be explained by the effects of sex hormones on the degree of lipid oxidation in the skin.The glutathione redox system is one of the most important antioxidant systems in the body [3,6]. However, we found no publications concerning activity of this system in the skin of healthy humans and patients with dermatoses. Some steroid hormones possess antioxidant properties [ 1,7], but their effects on activity of LPO and glutathione redox system in the skin are still unknown.Here we studied the effects of estradiol and testosterone on LPO and glutathione redox systems in the dermis and epidermis of intact rats. MATERIALS AND METHODSExperiments were performed on adult male and female (in the diestrus phase [5]) rats weighing 140-180 g. Each series was conducted on 6-9 animals. The rats were killed under ether anesthesia 5, 30, 60, and 90 min and 1, 2, 3, 6, 12, and 24 h after injection of hormones. Skin homogenates were prepared by the routine method [ 11 ]. The amplitude of slow flash of Fe2+-induced chemiluminescence (Hsv) and contents of thiobarbituric acid reactive substances (TBA)-reactive substances in the dermis and epidermis were determined as described previously [2,9]. The contents of reduced glutathione (GSH), glutathione reductase (GR), and glutathione peroxidase (GPx) were estimated spectrophotometrically [3]. Hormones were injected subcutaneously in doses of 1 and l0 mg/kg in 1 ml 20% ethanol. Protein concentration was measured by the method of Lowry [13]. The results were analyzed by Student's t test. RESULTSSeries I showed that the amplitude of slow flash of Fe2*-induced chemiluminescence (HsF) and the content of TBA-reactive substances in the dermis and epider-0007-4888/99/0012-1235522.00 +2000 Kluwer Academic/Plenum Publishers 1236 Bulletin of Experimental Biology and Medicine, N o-12, 1999 PHARtX4ACOLOGYAND TOXICOLOGY mis depended on the rate of free radical generation and their involvement in tissue metabolism.All parameters of LPO in the dermis of intact rats surpassed those in the epidermis; they were also higher in male rats than in females. The data suggest that the intensity of LPO in rat skin depends on the sex (i.e., on the content of sex hormones) and type of the ti...

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Section: Abstract: Skin; Dermis; Epidermis; Estradiol; Lipid Peroxidmentioning

confidence: 99%

Effects of sex steroid hormones on lipid peroxidation and glutathione antioxidant system in rat skin

1999

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“…Steroid hormones can regulate the antiperoxidation defense due to their effect on the glutathione redox-system expressed in almost all tissues [4]. However, we found no published data on the effect of glucocorticoids on the state and activity of its components in the skin.…”

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confidence: 95%

Effect of glucocorticoids and gestagens on glutathione redox-system in rat skin involved in dermatitis

2000

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In the model of experimental dermatitis the effect of hydrocortisone and two gestagens (endogenous progesterone and synthetic mecygestone) on the level of glutathione redox-system components (reduced glutathione, glutathione reductase, and glutathione peroxidase) in rat dermis and epidermis is characterized by different degree of activation of this system. Key Words: sMn; dermis; epidermis; allergic dermatitis; hydrocortisone; progesterone; mecygestone; gestagens; glutathione Long-term and intensive hormonal therapy of skin diseases even with local drugs is often accompanied by complications and relapses after discontinuation [6], which can be due to insufficient knowledge about the effects produced by hormones on the skin.The antiinflammatory effect of glucocorticoids is probably related to their capacity to inhibit lipid peroxidation (LPO); which is maintained at a certain level due to the action of prooxidant and antioxidant systems. Hydrocortisone inhibits LPO in tissues due to antioxidant activity [ 1,5]; however, the mechanisms of this effect are poorly understood.Steroid hormones can regulate the antiperoxidation defense due to their effect on the glutathione redox-system expressed in almost all tissues [4]. However, we found no published data on the effect of glucocorticoids on the state and activity of its components in the skin. Apart from the antioxidant effect similar to that of hydrocortisone [8], progesterone also produces an antiproliferative effect in dermatoses.Our aim was to compare the effects of hydrocortisone and two gestagens (endogenous progesterone and synthetic mecygestone) on the content of reduced glutathione (GSH) and activity of glutathione reductase (GR) and glutathione peroxidase (GPx) in rat dermis and epidermis in experimental dermatitis.

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“…Glutathione is an active substance involved in many redox processes [2,3,6,7]. Its levels are maximum in the kidneys, liver, brain, and peripheral blood erythrocytes [4].…”

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confidence: 99%

“…Its levels are maximum in the kidneys, liver, brain, and peripheral blood erythrocytes [4]. Glutathione protects the membrane and cytosol proteins containing thiol groups, and hemoglobin from oxidants, thus maintaining the structural stability of the red blood cells [3,8]. Glutathione reduction process is regulated, on the one hand, by glutathione reductase (GT) supplying NADPH to the cells, and on the other, is maintained by glutathione peroxidase (GP) neutralizing H 2 O 2 formed in redox reactions.…”

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confidence: 99%

Morphofunctional Characteristics of the Glutathione Cycle in Peripheral Blood Erythrocytes of Pregnant Women with a History of Cytomegalovirus Infection Exacerbation during Gestation

2014

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We studied factors influencing peripheral blood erythrocytes of pregnant women with exacerbation of cytomegalovirus infection during gestation. Cytomegalovirus infection was associated with a decrease in the content of reduced glutathione in erythrocytes and glutathione peroxidase, which leads to H2O2 accumulation in the blood. Exacerbation of cytomegalovirus infection was associated with reduction of glutathione reductase and SOD activities. Erythrocyte antioxidant function and the peripheral blood discocyte count decreased. Accumulation of degenerative erythrocytes is fraught with the risk of anemia in pregnant women.

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Detection and investigation of a protein factor concerned with the stimulation of disulfide reductase activity by cyclic AMP and other effectors

Cited by 5 publications

References 9 publications

Effects of sex steroid hormones on lipid peroxidation and glutathione antioxidant system in rat skin

Effects of sex steroid hormones on lipid peroxidation and glutathione antioxidant system in rat skin

Effect of glucocorticoids and gestagens on glutathione redox-system in rat skin involved in dermatitis

Morphofunctional Characteristics of the Glutathione Cycle in Peripheral Blood Erythrocytes of Pregnant Women with a History of Cytomegalovirus Infection Exacerbation during Gestation

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