Detection and measurement of fetomaternal haemorrhage: serum alpha-fetoprotein and the Kleihauer technique.

Lachman, E; Hingley, Susan M.; Bates, Gillian; Ward, A M; Stewart, Christopher; Duncan, Sheila L. B.

doi:10.1136/bmj.1.6073.1377

Cited by 40 publications

(23 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Currently, the Kleihauer Betke (KB) test is the most commonly used test to quantify fetomaternal hemorrhage (Lachman et al , 1977; Lenke et al , 1985). This acid elution test identifies fetal cells in the maternal circulation by the relative resistance of cells containing hemoglobin F to acid treatment.…”

Section: Introductionmentioning

confidence: 99%

Circulating cell‐free DNA levels increase variably following chorionic villus sampling

et al. 2010

View full text Add to dashboard Cite

Objective Cell-free fetal DNA (cffDNA) in maternal plasma results from degradation of fetal and/or placental cells. Our objective was to determine if chorionic villus sampling (CVS) causes increased release of fetal and/or maternal DNA. Methods Fifty-two pregnant women were recruited prior to CVS, performed for clinical indications, at 10 5/7 to 13 2/7 weeks. Maternal blood was collected before and within 15 minutes after CVS. cffDNA was extracted from plasma. Real-time polymerase chain reaction (PCR) amplification of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and the Y chromosome sequence DYS1 were used as measures of total and fetal DNA, respectively. All samples were analyzed in triplicate without knowledge of fetal gender. Results Sensitivity of DYS1 detection in male fetuses was 100% (n=30); specificity in female fetuses was 100% (n=22). While a majority of women had >50% post-procedure increases in both fetal and total DNA, some showed post-procedure decreases. However, overall median proportional increases were not statistically significant. Gestational age (GA), placental location, and individual CVS operator did not correlate with changes in DNA levels. Conclusions While there were no statistically significant overall changes in DNA levels after CVS, as-yet undiscovered variables may influence the extent of post-procedure release of cell-free DNA in the circulation of pregnant women.

show abstract

Section: Introductionmentioning

confidence: 99%

Circulating cell‐free DNA levels increase variably following chorionic villus sampling

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Polyacrylamide gel electrophoresis has revealed two common TC isotypes, M and X, and two rare variants, S and F (3,4 ), that may influence the cellular availability of vitamin B 12 (5,6 ). The phenotypic variability is a multifactorial phenomenon that probably includes cell-type-specific processing of translated TC (5 ), but the substitution of proline (P) for arginine (R) at codon 259 of the TC gene is the major determinant of the TC variability, at least in Caucasians (5,7 ), and affects TC concentrations Clinical Chemistry 49, No.…”

mentioning

confidence: 99%

Cell-free Fetal DNA in Maternal Circulation after Amniocentesis

et al. 2003

View full text Add to dashboard Cite

“…In five studies, FMH after FBS procedures was assessed by simultaneous combination of the Kleihauer technique and maternal ·-fetoprotein levels [3][4][5][6]13]. Some authors [5,6] have suggested that maternal ·-fetoprotein determination was more sensitive than the Kleihauer technique in the diagnostic of FMH following amniocentesis while other authors [3,4] have regarded these as two equivalent techniques. Similarly, the results of a previous study [13] have demonstrated that both techniques were comparable in the detection of FMH after cordocentesis.…”

Section: Discussionmentioning

confidence: 99%

“…Positive and negative controls were introduced for each test. A significant hemorrhage was considered to have occurred after cordocentesis if the difference in fetal bleeding before and after the procedure was 0.25 ml [5] or more. Using the number of fetal erythrocytes, intra-and interobserver variability was found to be 8.1 and 31%, respectively.…”

Section: Methodsmentioning

confidence: 99%

Detection and Measurement of Fetomaternal Hemorrhage following Diagnostic Cordocentesis

Chitrit

Caubel²,

Lusina³

et al. 1998

Fetal Diagn Ther

View full text Add to dashboard Cite

Objective: The aim of this study was to investigate the effect of cordocentesis on fetomaternal hemorrhage (FMH). Methods: One hundred and three diagnostic cordocenteses, without any other associated procedure, were performed at 23–40 weeks’ gestation. FMH was detected using the Kleihauer-Betke staining of maternal blood taken immediately before and after cordocentesis. Results: Significant FMH occurred after 40 (38.8%) of the 103 procedures. An increased risk of fetal bleeding was associated with both an anterior placenta (odds ratio (OR) 5.89; 95% confidence interval (CI) 2.27–15.3; p < 0.001) and a transplacental cordocentesis (OR 37.0; 95% CI 2.15–636; p < 0.001). The volume of FMH was greater after cordocentesis with an anterior placenta (90th percentile 6.20 ml) than after cordocentesis with a lateral (90th percentile 4.58 ml) or posterior placenta (90th percentile 1.35 ml) (p < 0.001). After fetal blood sampling, significant FMH occurred more frequently with a procedure duration of 3 min or more (OR 4.45; 95% CI 1.70–11.7; p = 0.002) and with two or more needle insertions (OR 4.65; 95% CI 1.80–12.1; p = 0.001). Conclusion: FMH following cordocentesis may be related to placental injuries. This event is influenced by placental location, procedure duration and the number of needle insertions.

show abstract

Detection and measurement of fetomaternal haemorrhage: serum alpha-fetoprotein and the Kleihauer technique.

Cited by 40 publications

References 4 publications

Circulating cell‐free DNA levels increase variably following chorionic villus sampling

Circulating cell‐free DNA levels increase variably following chorionic villus sampling

Cell-free Fetal DNA in Maternal Circulation after Amniocentesis

Detection and Measurement of Fetomaternal Hemorrhage following Diagnostic Cordocentesis

Contact Info

Product

Resources

About