Detection and molecular characterisation of bovine Enterovirus in Brazil: four decades since the first report

Abstract

It is suggested that bovine enteroviruses (BEV) are involved in the aetiology of enteric infections, respiratory disease, reproductive disorders and infertility. In this study, bovine faecal samples collected in different Brazilian states were subjected to RNA extraction, reverse transcription-polymerase chain reaction analysis and partial sequencing of the 5′-terminal portion of BEV. One hundred and three samples were tested with an overall positivity of 14.5%. Phylogenetic analysis clustered these BEV Brazilian samples into the Enterovirus F clade. Our results bring an important update of the virus presence in Brazil and contribute to a better understanding of the distribution and characterisation of BEV in cattle.

Molecular Epidemiology of Bovine Enteroviruses and Genome Characterization of Two Novel Bovine Enterovirus Strains in Guangxi, China

“…BEV is one of the most important pathogens causing digestive, respiratory, and reproductive tract diseases in cattle, which can be readily coinfected with BEV and other viruses, causing significant economic losses in the cattle industry ( 16 , 17 ). The prevalence of BEV has been reported worldwide, with positivity rates of 67% in Thailand, 14.5% in Brazil, 78% in Spain, 22.83% in Turkey, and 21.49% in Henan Province, China ( 10 , 14 , 18 – 20 ). However, so far, there have been no reports on the prevalence of BEVs in Guangxi Province, China, which is one of the main cattle and buffalo farming regions.…”

Serological Investigation of Bovine Enterovirus in Calves in Konya Province

“…Persistent diarrhea, respiratory disease, and enteritis are common pathological signs in cattle infected with BEV. Moreover, they do not have characteristic pathological lesions distinguishing BEV infection from other pathogens that cause similar clinical signs (Candido et al, 2019). It is highly resistant to environmental conditions (Birdane and Gür, 2019).…”

“…Bovine enterovirus (BEV) is an Enterovirus in the Picornaviridae family. Picornaviruses are small, nonmembrane, positive-stranded RNA viruses (Candido et al, 2019). The Picornaviridae family consists of 63 genera and 147 species.…”

Genome Sequence of a Brazilian Bovine Enterovirus

“…BEVs are endemic in cattle worldwide but, despite its large commercial cattle herd, data about BEV infection in Brazil are scarce. After a serological screening performed in the 1970s ( 3 ), the presence of these viruses determined through reverse transcriptase (RT)-PCR was described only in 2016 ( 4 ). Since then, no other data on BEV in Brazil have been published.…”

“…In a previous report of BEV identification in Brazil ( 4 ), the strains were classified as Enterovirus F species. However, the sequences analyzed were 180 nucleotides long and were not considered for inclusion in this phylogenetic analysis.…”