Detection and molecular characterization of porcine kobuvirus in piglets in 2009–2013 in northern Thailand

Chuchaona, Watchaporn; Khamrin, Pattara; Yodmeeklin, Arpaporn; Kongkaew, Aphisek; Vachirachewin, Ratchaya; Ushijima, Hiroshi; Maneekarn, Niwat

doi:10.1007/s11250-017-1298-4

Cited by 12 publications

(8 citation statements)

References 28 publications

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“…However, only 4% of the diarrhoeic pigs were not co-infected with other pathogens. Recent studies have found the prevalence of PKV-1 to be similar in diarrhoeic and healthy pigs [58][59][60][61], although, two of the studies showed that diarrheic suckling piglets was the most frequently infected group but the results were not statistically significantly different from the healthy piglets [58,59]. Altogether, these finding indicated that PKV-1 is not a significant primary pathogen in natural cases of diarrhoea in young piglets.…”

Section: Discussionmentioning

confidence: 88%

No evidence of enteric viral involvement in the new neonatal porcine diarrhoea syndrome in Danish pigs

et al. 2017

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BackgroundThe aim of this study was to investigate whether the syndrome New Neonatal Porcine Diarrhoea Syndrome (NNPDS) is associated with a viral aetiology. Four well-managed herds experiencing neonatal diarrhoea and suspected to be affected by NNPDS were included in a case-control set up. A total of 989 piglets were clinically examined on a daily basis. Samples from diarrhoeic and non-diarrhoeic piglets at the age of three to seven days were selected for extensive virological examination using specific real time polymerase chain reactions (qPCRs) and general virus detection methods.ResultsA total of 91.7% of the animals tested positive by reverse transcription qPCR (RT-qPCR) for porcine kobuvirus 1 (PKV-1) while 9% and 3% were found to be positive for rotavirus A and porcine teschovirus (PTV), respectively. The overall prevalence of porcine astrovirus (PAstV) was 75% with 69.8% of the PAstV positive pigs infected with PAstV type 3. No animals tested positive for rotavirus C, coronavirus (TGEV, PEDV and PRCV), sapovirus, enterovirus, parechovirus, saffoldvirus, cosavirus, klassevirus or porcine circovirus type 2 (PCV2). Microarray analyses performed on a total of 18 animals were all negative, as were eight animals examined by Transmission Electron Microscopy (TEM). Using Next Generation de novo sequencing (de novo NGS) on pools of samples from case animals within all herds, PKV-1 was detected in four herds and rotavirus A, rotavirus C and PTV were detected in one herd each.ConclusionsOur detailed analyses of piglets from NNPDS-affected herds demonstrated that viruses did not pose a significant contribution to NNPDS. However, further investigations are needed to investigate if a systemic virus infection plays a role in the pathogenesis of NNPDS.

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Section: Discussionmentioning

confidence: 88%

No evidence of enteric viral involvement in the new neonatal porcine diarrhoea syndrome in Danish pigs

et al. 2017

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“…Porcine Kobuviruses , infecting only pigs, are generally closely related. However, after the discovery of porcine-like Bovine Kobuvirus in pigs, the interspecies transmission of Kobuviruses cannot be excluded ( Chuchaona et al, 2017 ). Porcine Kobuvirus infection in Serbia has never been investigated, though the very high prevalence in the neighboring countries suggests the high likelihood of the presence in Serbia too.…”

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confidence: 99%

Molecular detection and characterization of Porcine Kobuvirus in domestic pigs and wild boars in Serbia

Milićević¹,

Kureljušić²,

Maksimović-Zorić³

et al. 2020

Research in Veterinary Science

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Porcine Kobuvirus (PKV) infection is very common in pigs throughout the world. Since it has never been investigated in Serbia, to contribute to the knowledge of Porcine Kobuvirus , its role, and distribution, we tested 200 samples from domestic pigs and wild boars. From domestic pigs, 10 fecal, 22 spleen and 68 serum samples, and 100 spleen samples from wild boars were tested. The virus prevalence determined by real-time RT-PCR in domestic pigs was 22% and in wild boars 6%. The phylogenetic analysis of 3D region revealed that Serbian strains are closest related to the Hungarian strain from wild boar from 2011. This is the first report on PKV in Serbia in domestic pigs and wild boars, implying its wide circulation. Although the infection could not be directly related to any clinical manifestation, the frequency of virus found in feces suggests viral affinity to the gastrointestinal tract. However, due to the rather ubiquitous presence of PKV, the clinical and pathological assessment have to be considered when PKV infection is diagnosed.

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“…Porcine viral diarrhea disease seriously endangers the development of the pig industry, and leads to signi cant economic losses for pig farmers worldwide [7]. Clinically, the complexity of the disease has increased.…”

Section: Discussionmentioning

confidence: 99%

The Multiple Infections of Porcine Diarrhea Viruses in Local Area Based on A Luminex xTAG Multiplex Detection Method

Liu

Shi

et al. 2020

Preprint

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Background Porcine viral diarrhea can cause great damage to the pig industry. However, multiple infections have contributed to the poor control of diarrhea, which has also resulted in great difficulties in determining the main pathogenic factors. Methods A Luminex xTAG multiplex detection method was developed for the detection of 11 viral diarrhea pathogens, which allows for the simultaneous qualitative and quantitative detection of viral diarrhea pathogens in clinical samples. A total of 518 porcine stool specimens were collected from 9 pig herds in Shanghai, China from 2015 to 2017, and the pathogen spectrums and co-infections were analyzed. Results The minimum detection rate of the Luminex xTAG multiplex detection method was at least 10 times higher than the traditional PCR method. Of the 518 diarrhea samples, PEDV was found in 17.57% (91/518), PKoV in 40.35% (209/518), PAstV in 26.64% (138/518), PSV in 15.06% (78/518), PoSaV in 13.13% (68/518), PTV in 5.21% (27/518), PDCoV in 4.83% (25/518), PoRV in 3.28% (17/518), TGEV in 3.09% (16/518), PToV in 1.93% (10/518), and BVDV in 1.74% (9/518), respectively. Furthermore, multiple infections were commonly seen, with positive rate of 35.14%. Infection pattern of the viral diarrheal pathogens in a specific farm was changing, and different farms had the various diarrhea infection patterns. A longitudinal investigation showed that PEDV was still the key pathogen which was closely related to the death of diarrhea piglets. Other pathogens might play synergistic roles in the pathogenesis of diarrhea disease. Conclusions Here we provided a Luminex xTAG multiple detection method for viral diarrhea pathogen infection in clinical, which was more sensitive and specific than general multiplex PCR method. Furthermore, the surveillance confirmed high infection rate of PKoV, but PEDV was still the key pathogen and multiple pathogens synergistically complicated the infection status in southern China, suggesting that controlling porcine diarrhea might be more complex than previously thought. A better understanding of viruses that cause diarrhea in piglets will aid in better preventing and controlling epidemics of viral porcine diarrhea.

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Detection and molecular characterization of porcine kobuvirus in piglets in 2009–2013 in northern Thailand

Cited by 12 publications

References 28 publications

No evidence of enteric viral involvement in the new neonatal porcine diarrhoea syndrome in Danish pigs

No evidence of enteric viral involvement in the new neonatal porcine diarrhoea syndrome in Danish pigs

Molecular detection and characterization of Porcine Kobuvirus in domestic pigs and wild boars in Serbia

The Multiple Infections of Porcine Diarrhea Viruses in Local Area Based on A Luminex xTAG Multiplex Detection Method

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