Detection and molecular characterization of Cryptosporidium spp. in captive canaries (Serinus canaria) using different diagnostic methods

Camargo, Vinícius da Silva; Santana, Bruna Nicoleti; Ferrari, Elis Domingos; Nakamura, Alex Akira; Nagata, Walter Bertequini; Nardi, Ana Rita Moraes; Meireles, Marcelo Vasconcelos

doi:10.1590/s1984-296120180010

Cited by 14 publications

(9 citation statements)

References 34 publications

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“…No pathology. Abe and Matsubara, 2015 C. avium Buderigar, Cockatiel P Melopsittacus undlates Nymphicus hollandicus China KM267556 none none unknown Zhang et al (2015) C. avium * Red-crowned parakeet, Chicken, Budgerigar P Cyanoramphus novaezealandiae, Gallus gallus , Melopsittacus undlates Czechia KU058875-78 KU058879-82 KU058883-86 none Holubová et al (2016) C. avium Canary P Serinus canaria Brazil MG832882 none none none Camargo et al (2018) C. avium * Cockatiel P , Chicken* Nymphicus hollandicus , Gallus gallus China JQ246415 JQ320301 JQ798893 none Cui et al (2018) C. avium * Mallard, Chicken, Pheasant W,A Anas platryrhynchos , Gallus gallus , Phasianus colchicus Czechia none none none none Holubová et al (2018) C. avium Red-crowned parakeet, Budgerigar P Cyanoramphus novaezealandiae, ...…”

Section: Resultsmentioning

confidence: 99%

Cryptosporidium cf. avium in an inland-bearded dragon (Pogona vitticeps) – A case report and review of the literature

Koehler¹,

Scheelings²,

Gasser³

2020

International Journal for Parasitology: Parasites and Wildlife

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Here, we report the first case of Cryptosporidium cf. avium from an inland bearded dragon ( Pogona vitticeps ) from a wildlife sanctuary in Victoria, Australia. Molecular characterisation was conducted by PCR-coupled sequencing of regions in the small subunit of nuclear RNA ( SSU ), actin and large subunit of nuclear RNA ( LSU ) genes. The sequences obtained grouped with those of C . ornithophilus and other C. avium genotypes/variants originating from reptiles or birds. We discuss this case in relation to the current state of knowledge of C. avium of birds and reptiles, considering provenance and environment (agricultural, pet industry, wildlife, zoo or wildlife park) as well as clinical context, and pathological changes associated with cryptosporidiosis in these host animals.

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Section: Resultsmentioning

confidence: 99%

Cryptosporidium cf. avium in an inland-bearded dragon (Pogona vitticeps) – A case report and review of the literature

Koehler¹,

Scheelings²,

Gasser³

2020

International Journal for Parasitology: Parasites and Wildlife

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“…affect the digestive or respiratory tract of birds; and has increasingly been reported in many species of birds such as Java sparrows (8), canaries (9), psittacines (10), companion birds (11), and several other birds (12). Among these birds, domestic pigeons may deserve more attention due to their wide distribution in cities.…”

Section: Introductionmentioning

confidence: 99%

High Prevalence of Cryptosporidium meleagridis in Domestic Pigeons (Columba livia domestica) Raises a Prospect of Zoonotic Transmission in Babylon Province, Iraq

Altamimi¹,

Al-Zubaidi

2020

Iraqi J. Vet. Med.

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Cryptosporidium is one of the most common protozoan’s parasites with remarkable infectivity of a wide range of animals, including mammals and birds. Domestic pigeons (Columba livia domestica) act as a potential reservoir for several species of Cryptosporidium because they live in close proximity to humans. This study was conducted to assess the genetic diversity of Cryptosporidium in domestic pigeons in Iraq. A total of one hundred samples obtained from feces of domestic pigeons in Babylon province were included. After being exposed to microbial examination, all fecal samples were subsequently screened by nested polymerase chain reaction (PCR) for the possible recognition of Cryptosporidium species. Microscopy tests detected only 14/100 (14%) of infection with Cryptosporidium, while molecular tests detected 21/100 (21%) of the same targeted parasite. Sequencing experiments showed a high prevalence of C. meleagridis with 13/21 (61.90%), followed by C. baileyi with 7/21 (33.33%), while only one infection was detected with C. hominis (1/21) (4.76%). No co-infection with mixed Cryptosporidium spp. was observed, and sex factor was not found to affect the infection rate. In conclusion, this study informed a high prevalence of C. meleagridis in domestic pigeons than both C. baileyi and C. hominis, respectively, signifying a higher zoonotic potential of C. meleagridis between domestic pigeons and their handlers. This finding may raise more questions with regard to the increasing infectivity of C. meleagridis in human. This is the first important screening study in Iraq that uses molecular methods for the detection of Cryptosporidium in domesticated pigeons.

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“…The PCR primers used in this study were previously known to have the ability to amplify a segment of the Cryptosporidium sp. 18S rRNA gene (Alkhaled, 2017;Ayinmode et al, 2018;Ayinmode et al, 2017;Camargo et al, 2018;Lucio et al, 2016). But our results revealed that Colpodella sp.…”

Section: Discussionmentioning

confidence: 45%

Cross-genera amplification and identification of Colpodella sp. with Cryptosporidium primers in fecal samples of zoo felids from northeast China

Hussain

Li²,

Bukhari

et al. 2023

Braz. J. Biol.

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The protozoans include many intracellular human pathogens. Accurate detection of these pathogens is necessary to treat the diseases. In clinical epidemiology, molecular identification of protozoan is considered a more reliable and rapid method for identification than microscopy. Among these protozoans, Cryptosporidium considered being one of the important water-borne zoonotic pathogens and a major cause of a diarrheal disease named cryptosporidiosis in humans, domestic animals, and wild animals. This study was aimed to identify Cryptosporidium in zoo felids (N= 56) belonging to different zoo of China, but accidentlly Colpodella was encountered in the zoo felids sample and phylogenetic data confirmed this unexpected amplification from fecal samples using two-step nested-PCR. Phylogenetic analysis revealed the fact about the specific primers used previously by many researchers and cross-genera amplification. We came to know that genetically sequenced amplicon gives more accurate identification of species. This study suggests more investigation on Colpodella which has been neglected previously but gains the attention of researchers after identified from humans and animals and has been known to correlate with neurological symptoms in patients.

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Detection and molecular characterization of Cryptosporidium spp. in captive canaries (Serinus canaria) using different diagnostic methods

Cited by 14 publications

References 34 publications

Cryptosporidium cf. avium in an inland-bearded dragon (Pogona vitticeps) – A case report and review of the literature

Cryptosporidium cf. avium in an inland-bearded dragon (Pogona vitticeps) – A case report and review of the literature

High Prevalence of Cryptosporidium meleagridis in Domestic Pigeons (Columba livia domestica) Raises a Prospect of Zoonotic Transmission in Babylon Province, Iraq

Cross-genera amplification and identification of Colpodella sp. with Cryptosporidium primers in fecal samples of zoo felids from northeast China

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