Detection and molecular diversity of Giardia duodenalis and Cryptosporidium spp. in sheltered dogs and cats in Northern Spain

Gil, Horacio; Cano, Lourdes; Lucio, Aida de; Bailo, Begoña; Mingo, Marta Hernández de; Cardona, Guillermo A.; Fernández-Basterra, José A.; Aramburu-Aguirre, Juan; López-Molina, Nuria; Carmena, David

doi:10.1016/j.meegid.2017.02.013

Cited by 85 publications

(86 citation statements)

References 52 publications

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“…Sequence typing was successful in only 6/13 canine samples, although an effort was made to type at four different marker genes. Expectedly, the most successful PCR method in the present study was at the multicopy SSUrDNA gene locus compared to the typing attempts at the singly gene copy loci tpi, bg and gdh as reported previously (Adell‐Aledon et al, ; Gil et al, ). Low parasite numbers in the analysed faecal samples likely contributed to this low typing success.…”

Section: Discussionsupporting

confidence: 63%

Giardia duodenalis in small animals and their owners in Germany: A pilot study

Rehbein

Klotz

Ignatius

et al. 2018

Zoonoses and Public Health

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Giardia duodenalis is a relevant gastrointestinal protozoan pathogen of humans and animals. This species complex consists of eight genetically different assemblages. Assemblages A and B are pathogenic to humans and pets, thus confer zoonotic potential. The risk of zoonotic transmission has been controversially discussed. The aim of this monocentric cross‐sectional pilot study was to investigate G. duodenalis assemblages in humans and pets living in common households in Berlin/Brandenburg (Germany). Samples from dogs, cats and humans sharing the same households were screened for Giardia infection by antigen‐detecting assays. All human samples were additionally analysed by a Giardia‐specific qPCR. Cyst quantification and sequences of different gene loci (triosephosphate isomerase (tpi), glutamate dehydrogenase (gdh), β‐giardin (bg) and for dogs SSUrDNA) were analysed. A total of 38 households (31 households with dogs and seven with cats) with 69 human individuals participated in the study. Initial antigen‐detecting assays revealed Giardia‐positive results for 13 (39%) canine, one (14%) feline and one human sample. Reanalysis of the human samples by qPCR revealed two more positive specimens (4%). Two of these three samples were identified as assemblage B at all tested loci. Success rate of assemblage typing for pet samples was generally low and comprised mainly the SSUrDNA locus only. Overall, six of 13 Giardia‐positive canine samples were typable (2× A, 1× co‐infection: A and B, 1× C; 2× D). One pair of samples (dog and human) from the same household had a similar but not identical assemblage B sequence at tpi locus. Assemblage A was also detected in the dog specimen, which hampered sequence analysis. In conclusion, although exhibiting limitations due to the sample size, our study highlights the need for better and standardized typing tools to distinguish G. duodenalis strains with higher resolution in order to perform proper case–control studies for a realistic estimation of zoonotic risk.

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Section: Discussionsupporting

confidence: 63%

Giardia duodenalis in small animals and their owners in Germany: A pilot study

Rehbein

Klotz

Ignatius

et al. 2018

Zoonoses and Public Health

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“…Despite the results of the current study, in some researches the frequency of Cryptosporidium infection was higher in young dogs (21,31,33,46). Some studies in comparison to the current study indicated that infection rates in female dogs were higher than male dogs, which may be due to reduced immunity at certain periods in female physiologic cycle (32).…”

Section: Discussioncontrasting

confidence: 83%

Molecular Study of Cryptosporidium spp. in Dogs from Southwest of Iran

Tavalla

Kord

Abdizadeh

et al. 2017

Jundishapur J Microbiol

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Background: Cryptosporidium is a protozoan parasite that effects rodents, dogs, calves, humans, and cats. Infection with this parasite is known as cryptosporidiosis. Cryptosporidium spp. may induce clinical or subclinical signs in infected hosts. In the life cycle of this parasite infected dogs freely living in urban and rural areas of Khuzestan province are the definitive hosts that should be considered as a real problem in public health for humans. Objectives: This study aimed at determining the frequency of cryptosporidiosis in dogs in southwest of Iran. Methods: Overall, 350 fresh fecal samples were collected from domestic dogs living in 43 villages, from June 2012 to September 2013. All samples were investigated by Sheather's concentration method and fecal smears were stained with modified Ziehl-Neelsen followed by light microscope examination, and polymerase chain reaction (PCR). Results:The results revealed that frequency of Cryptosporidium infection was 8% and 12.3%, using direct smear and molecular method, respectively. Conclusions: The present findings indicated that domestic dog feces from southwest of Iran may contain zoonotic parasites such as Cryptosporidium spp. and may be a potential risk for humans and other animals, especially when they contaminate the environment. The role of dogs as source of human infection should be investigated by further studies.

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“…Our results indicate that the age may affect the rates of infection through the significant high prevalence of Cryptosoridium in the young dogs than in adult dogs using both methods. Similar research were reported in dogs (5,20). This could be attributed to lower resistance in the young compared to older animals.…”

Section: Phylogenetic Analysissupporting

confidence: 88%

Traditional and Molecular Study of Cryptosoporidium Spp. In Domestic Dogs in Baghdad City,iraq

Faraj¹

2019

Iraqi J. Agric. Sci.

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The aim of this study to investigate the prevalence of Cryptosoridium spp. in domestic dogs in Baghdad city, by using microscopic examination(flotation and staining) and molecular techniques .The results revealed that the rate of Cryptosporidium infection was 15% by microscopic examination and 28.6% using PCR, our study showed that the infection rate was 8.5% in males and 21.42% in females by microscopic examination , while the infection rate by using PCR in males and females was 21.42%, 35.71% respectively. The percentage of infection in the age groups <1 year and >1 year was 20%, 10% respectively by microscopic examination, while by using molecular assay the rate of infection in the age groups <1 year was 40% and 17.14% in >1 year. This is the first study in Iraq to detect Cryptosoridium spp. in domestic dogs using molecular technique.

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Detection and molecular diversity of Giardia duodenalis and Cryptosporidium spp. in sheltered dogs and cats in Northern Spain

Cited by 85 publications

References 52 publications

Giardia duodenalis in small animals and their owners in Germany: A pilot study

Giardia duodenalis in small animals and their owners in Germany: A pilot study

Molecular Study of Cryptosporidium spp. in Dogs from Southwest of Iran

Traditional and Molecular Study of Cryptosoporidium Spp. In Domestic Dogs in Baghdad City,iraq

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