Detection and molecular identification of Anaplasma phagocytophilum and Babesia spp. infections in Hyalomma aegyptium ticks in Tunisia

Rjeibi, Mohamed Ridha; Amairia, Safa; Mhadhbi, Moez; Rekik, Mourad; Gharbi, Mohamed

doi:10.1007/s00203-022-02995-7

Cited by 6 publications

(3 citation statements)

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“…Genetic markers used in the characterization of A. phagocytophilum in Africa include the msp2 , msp4 , citrate synthase ( gltA ), groEL , 16S rRNA and 23S rRNA genes [ 11 , 14 , 22 , 50 , 64 , 87 , 88 , 89 , 90 , 91 , 92 ] ( Table 1 ). In Tunisia, A. phagocytophilum was detected in 0.6% of cattle using a duplex PCR assay that amplified the msp2 gene [ 64 ].…”

Section: Molecular Epidemiology and Genetic Diversity Of An...mentioning

confidence: 99%

“…In Tunisia, A. phagocytophilum was detected in 0.6% of cattle using a duplex PCR assay that amplified the msp2 gene [ 64 ]. The organism was also detected in 13.6% of Hyalomma aegyptium ticks obtained from tortoises in the country using a nested PCR that amplified a 641 bp fragment of the 16S rRNA gene [ 89 ]. Sequence analysis identified two 16S rRNA gene variants of A. phagocytophilum in Hy.…”

Section: Molecular Epidemiology and Genetic Diversity Of An...mentioning

confidence: 99%

“…Sequence analysis identified two 16S rRNA gene variants of A. phagocytophilum in Hy. aegyptium that shared 99.7% sequence similarity and differed by two nucleotide substitutions [ 89 ]. Other research in the country detected A. phagocytophilum from the spleen of a wild rodent Rattus rattus using 16S rRNA gene PCR and sequencing [ 90 ].…”

Section: Molecular Epidemiology and Genetic Diversity Of An...mentioning

confidence: 99%

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Anaplasma Species in Africa—A Century of Discovery: A Review on Molecular Epidemiology, Genetic Diversity, and Control

Kolo

2023

Pathogens

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Anaplasma species, belonging to the family Anaplasmataceae in the order Rickettsiales, are obligate intracellular bacteria responsible for various tick-borne diseases of veterinary and human significance worldwide. With advancements in molecular techniques, seven formal species of Anaplasma and numerous unclassified species have been described. In Africa, several Anaplasma species and strains have been identified in different animals and tick species. This review aims to provide an overview of the current understanding of the molecular epidemiology and genetic diversity of classified and unclassified Anaplasma species detected in animals and ticks across Africa. The review also covers control measures that have been taken to prevent anaplasmosis transmission on the continent. This information is critical when developing anaplasmosis management and control programs in Africa.

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Section: Molecular Epidemiology and Genetic Diversity Of An...mentioning

confidence: 99%

Section: Molecular Epidemiology and Genetic Diversity Of An...mentioning

confidence: 99%

Section: Molecular Epidemiology and Genetic Diversity Of An...mentioning

confidence: 99%

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Anaplasma Species in Africa—A Century of Discovery: A Review on Molecular Epidemiology, Genetic Diversity, and Control

Kolo

2023

Pathogens

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Tick infestation in spur-thighed tortoise population: a pilot study for unraveling epidemiological patterns and demographic consequences

Segura,

Rafael,

Vaz-Rodrigues

et al. 2023

Exp Appl Acarol

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Ectoparasites, such as ticks, modulate host population dynamics by impacting demographic traits. They transmit infectious agents among their hosts, posing a critical threat to animal and public health. This study aimed to characterize and analyze the Hyalomma aegyptium infestation on one of its main hosts, the spur-thighed tortoise, its effects on demographic traits, and to determine the diversity of infectious agents present in both ticks and tortoises in the Maamora forest (northwestern Morocco). Our results show that 100% of the tortoises were parasitized by adult ticks in spring, an infestation intensity of 4 ticks/tortoise (5.1 and 3.6 ticks/tortoise in males and females, respectively; 4.2 and 3.3 ticks/tortoise in gravid and non-gravid females, respectively) and an abundance ranging from 1 to 12. Although without significant differences, male tortoises had higher tick abundances than females. The interaction of tortoise sex and body condition was significantly related to tick abundance, male body condition decreased with higher tick abundance in contrast to females. Nevertheless, the interaction of body condition and reproductive stage of females was not significantly related to tick abundance. Gravid females were significantly associated with tick abundance, showing a slightly higher infestation than non-gravid females. Molecular analysis of pooled tick samples revealed the presence of Ehrlichia ewingii, Candidatus Midichloria mitochondrii, and Rickettsia africae, with a minimum infection rate of 0.61 to 1.84%. However, blood sample analysis of the tortoises was infectious agent-free, pinpointing a lack of significant health problems. Given the possible effect on the transmission of zoonotic diseases by spur-thighed tortoises associated with their frequent collection as pets, it should be surveyed to control possible human health problems. In conservation terms, as a long-lived species, the role of tick infestation in demographic traits might be included in the management and conservation programs of spur-thighed tortoises.

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Molecular survey of Anaplasma phagocytophilum and related variants in water buffaloes: The first detection of Anaplasma phagocytophilum-like 1

Şahin

Erol

Düzlü

et al. 2023

Comparative Immunology, Microbiology and Infectious Diseases

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Detection and molecular identification of Anaplasma phagocytophilum and Babesia spp. infections in Hyalomma aegyptium ticks in Tunisia

Cited by 6 publications

References 47 publications

Anaplasma Species in Africa—A Century of Discovery: A Review on Molecular Epidemiology, Genetic Diversity, and Control

Anaplasma Species in Africa—A Century of Discovery: A Review on Molecular Epidemiology, Genetic Diversity, and Control

Tick infestation in spur-thighed tortoise population: a pilot study for unraveling epidemiological patterns and demographic consequences

Molecular survey of Anaplasma phagocytophilum and related variants in water buffaloes: The first detection of Anaplasma phagocytophilum-like 1

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