Detection and Phylogeny of Beak and Feather Disease Virus and Avian Polyomavirus in Psittacine Pet Birds in Turkey

Altan, Eda; Eravci, Ebru; Cizmecigil, Utku Y.; Yıldar, Esma; Aydın, Özge; Turan, Nuri; Özsoy, Serhat; Tekelioglu, Kaan B.; Kurt, Tugba; Özsemir, Kübra Gerbaga; Altan, Erbulent; Yılmaz, Hüseyin

doi:10.1053/j.jepm.2016.06.012

Cited by 13 publications

(9 citation statements)

References 22 publications

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“…DNA samples were maintained at −20°C until molecular testing was initiated (1). DNA concentration was then measured using a NanoDrop ND-1000 spectrophotometer (Thermo Scientific, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Viral DNA was extracted from the dropping swabs using a PureLink Genomic DNA Kit (Invitrogen, USA) as instructed by the manufacturer. DNA samples were maintained at −20°C until molecular testing was initiated ( 1 ). DNA concentration was then measured using a NanoDrop ND-1000 spectrophotometer (Thermo Scientific, USA).…”

Section: Methodsmentioning

confidence: 99%

“…APV infection, also known as budgerigar fledgling polyomavirus (BFPyV) disease and psittacine polyomavirus was reported both in budgerigars ( Melopsittacus undulatus ) ( 15 ) and cockatoos ( Cacatuidae ) ( 1 ). APV is assigned to the Polyomaviridae family and Avipolyomavirus genus and classified as a non-enveloped virus with an icosahedral viral capsid containing double-stranded DNA.…”

Section: Introductionmentioning

confidence: 99%

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Investigation and sequence analysis of avian polyomavirus and psittacine beak and feather disease virus from companion birds in eastern Turkey

Adıgüzel

Timurkan

Cengi̇z

2020

Journal of Veterinary Research

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IntroductionAvian polyomavirus (APV) and psittacine beak and feather disease virus (PBFDV) induce contagious and persistent diseases that affect the beaks, feathers, and immune systems of companion birds. APV causes hepatitis, ascites, hydropericardium, depression, feather disorders, abdominal distension, and potentially death. PBFDV can induce progressive beak deformity, feather dystrophy, and plumage loss. We conducted the first prevalence survey of both APV and PBFDV infections in companion birds in eastern Turkey.Material and MethodsA total of 113 fresh dropping samples from apparently healthy companion birds were collected in a random selection. The dropping samples were analysed for PBFDV and APV by PCR. Positive samples were sequenced with the Sanger method. The sequence was confirmed through alignment and the phylogenetic tree generated through the maximum likelihood method computationally.ResultsPBFDV and APV were detected in a respective 48.7% and 23.0% of samples. Coinfection was found in 12.4% of the samples, these all being from budgerigars (Melopsittacus undulatus). APV and PBFDV were detected in budgerigar and cockatiel (Nymphicus hollandicus) samples.ConclusionThis report provides a foundation for future studies on the influence of these viruses on the health of companion birds. These high positive rates for both pathogens emphasise that healthy M. undulatus and N. hollandicus in eastern Turkey may be prone to the emergence and spread of APV and PBFDV with subclinical potential.

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“…DNA samples were maintained at −20°C until molecular testing was initiated (1). DNA concentration was then measured using a NanoDrop ND-1000 spectrophotometer (Thermo Scientific, USA).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Investigation and sequence analysis of avian polyomavirus and psittacine beak and feather disease virus from companion birds in eastern Turkey

Adıgüzel

Timurkan

Cengi̇z

2020

Journal of Veterinary Research

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“…Initially the birds appeared dull and anorexic, abnormal feathers, skin discoloration, and abdominal distension were the most common clinical observations. Clinical signs for APV in young parrots are variable and mostly depend on the species and the age and deaths have been reported without any premonitory signs of the disease in budgrigars (Altan et al 2016). In the present study the birds died within 7 days of presentation.…”

Section: Discussionmentioning

confidence: 46%

“…Lane M: 100-bp DNA ladder, Lane N: Negative control similar feather disorders and cannot be differentiated by clinical diagnosis. PCR is used as a highly sensitive method, capable of detecting specific DNA sequences in various biological samples and has been used extensively in the diagnosis as well as confirmation of APV and PBFDV infection (Altan et al 2016;Dolz et al 2013;Ogawa et al 2005). Ogawa et al (2005) used duplex shuttle PCR for the differential diagnosis of APV and PBFDV in psittacine birds.…”

Section: Discussionmentioning

confidence: 99%

First detection and molecular characterization of avian polyomavirus in young parrots in Pakistan

et al. 2019

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Avian polyomavirus (APV) infection, also called as budgerigar fledgling disease (BFD) causes various health problems in many psittacine species which may cause untimely death. The aims of this study were to investigate, for the first time, the detection, molecular characterization and phylogenetic analysis of avian polyomavirus (APV) in Pakistani psittacine birds. In an aviary a disease similar to APV was found and 90% of the nestlings died within a few weeks. Seven to ten-day-old parrot nestlings (n = 3) from the aviary were presented with feather abnormalities, plumage defect and were clinically depressed. Birds died at 11th, 14th and 16th day of age. Samples of hearts, livers, spleen, feathers and kidneys were collected from the dead birds. Samples were analyzed for the presence of APV DNA by using PCR. APV VP1 gene was partially sequenced, and phylogenetic analysis was performed. The APV strain was similar to those previously reported in other areas of the world. The results of this investigation indicate presence of a high frequency of APV infections in psittacine birds in Pakistan.

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Evidence of circulation of beak and feather disease virus in captive psittacine and non-psittacine birds in Bangladesh

2022

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Detection and Phylogeny of Beak and Feather Disease Virus and Avian Polyomavirus in Psittacine Pet Birds in Turkey

Cited by 13 publications

References 22 publications

Investigation and sequence analysis of avian polyomavirus and psittacine beak and feather disease virus from companion birds in eastern Turkey

Investigation and sequence analysis of avian polyomavirus and psittacine beak and feather disease virus from companion birds in eastern Turkey

First detection and molecular characterization of avian polyomavirus in young parrots in Pakistan

Evidence of circulation of beak and feather disease virus in captive psittacine and non-psittacine birds in Bangladesh

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