Detection and quantification of ochratoxin A and deoxynivalenol in barley grains by GC-MS and electronic nose

Development of a recombinant Fab-fragment based electrochemical immunosensor for deoxynivalenol detection in food samples

“…Results obtained during the evaluation of the matrix effect clearly showed that the developed electrochemical immunosensor for DON detection is extremely robust and insensitive to sample matrix interference (wheat, breakfast cereal and baby-food). Although other techniques such as SPR (Tüdös et al, 2003;Schnerr et al, 2002), fluorescent array biosensor (Ngundi et al, 2006) and electronic olfactory system (Olsson et al, 2002) were proposed for the detection of DON in food samples, to our knowledge none of them was challenged with complex matrices such as baby-food and breakfast cereals. We demonstrated here that the detection of DON with the developed immunosensor was possible in all these matrices.…”

Rapid Methods as Analytical Tools for Food and Feed Contaminant Evaluation: Methodological Implications for Mycotoxin Analysis in Cereals

“…Some are advanced enough for field studies and have already reached the stage of commercialization, some are at a transition phase between research and application to analysis of food/feed samples, other still have to face the challenge of validation by multiple laboratories. A list of the emerging rapid methods for mycotoxin analysis is reported in Table 2 Keshri & Magan, 2000;Olsson et al, 2002;Presicce et al, 2006;Cheli et al, 2009b;Campagnoli et al, 2011. (Maragos, 2004;Krska & Welzig, 2006;Zeng et al, 2006;Goryacheva et al, 2007;Maragos & Busnam, 2010). The most known rapid screening methods for mycotoxin detection, especially for the screening of raw materials, are antibody-based methods, ELISA test.…”

“…Tests were conducted on DON levels in barley and wheat. Positive correlation was found between electronic nose data and reference concentration of DON (Olsson et al, 2002). However the performance of the regression model on prediction was quite low (PRESS =0.65, R 2 =0.63, adjR 2 =0.63) (Tognon et al, 2005;Dell'Orto et al, 2007).…”

“…Further developments of studies carried out with EN technology have been made in order to evaluate the possibility of using fungal volatile metabolites as indicators of mycotoxin presence (Campagnoli et al, 2009b). Results from a study carried out on naturally contaminated barley samples showed that it was possible to use volatile compounds to predict whether the OTA level in samples was below or above 5 μg/kg; seven of 37 samples were misclassified (Olsson et al, 2002). EN analysis enabled correct classification of naturally contaminated maize with aflatoxins (Campagnoli et al, 2009a(Campagnoli et al, , 2009bCheli et al, 2009b).…”

05/01826 Microbial volatile organic compounds as indicators of fungi. Can an electronic nose detect fungi in indoor environments?

“…In 2002, Olsson et al [52] compared the presence of mycotoxins with the presence of VOCs in grain samples. The samples were evaluated with regard to moisture content, fungal contamination, ergosterol content and levels of two mycotoxins: ochratoxin A and deoxynivalenol.…”