Detection and quantification of vitamins in microliter volumes of biological samples by LC‐MS for clinical screening

Plants are regularly colonised by fungi and bacteria, but plant‐inhabiting microbes are rarely considered in studies on plant–herbivore interactions. Here we show that young gypsy moth (Lymantria dispar) caterpillars prefer to feed on black poplar (Populus nigra) foliage infected by the rust fungus Melampsora larici‐populina instead of uninfected control foliage, and selectively consume fungal spores. This consumption, also observed in a related lepidopteran species, is stimulated by the sugar alcohol mannitol, found in much higher concentration in fungal tissue and infected leaves than uninfected plant foliage. Gypsy moth larvae developed more rapidly on rust‐infected leaves, which cannot be attributed to mannitol but rather to greater levels of total nitrogen, essential amino acids and B vitamins in fungal tissue and fungus‐infected leaves. Herbivore consumption of fungi and other microbes may be much more widespread than commonly believed with important consequences for the ecology and evolution of plant–herbivore interactions.

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“…B vitamins were analysed from the extract by LC‐MS/MS using a method described by Khaksari et.al. (2018) with modifications (Table S3).…”

Section: Methodsmentioning

confidence: 99%

Herbivory meets fungivory: insect herbivores feed on plant pathogenic fungi for their own benefit

et al. 2020

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“…However, all of these methods are not confirmatory approaches, because they cannot provide direct proof of the analytes structures. Undoubtedly, the introduction of liquid chromatography-mass spectrometry (LC-MS) [16] and gas chromatography-mass spectrometry (GC-MS) [17] was a major step towards analysis of multivitamins in different matrices. The obvious advantage of MS is that it is a global quantum detector of any compound.…”

Section: Introductionmentioning

confidence: 99%

Dilute-and-shoot-based direct nano-electrospray ionization tandem mass spectrometry as screening methodology for multivitamins in dietary supplement and human urine

Amer

Zarad

El-Gendy

et al. 2020

Journal of Advanced Research

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Rapid Determination of Water-Soluble Vitamins in Human Serum by Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry

Zhang,

Cai

et al. 2024

ACS Omega

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Water-soluble vitamins play essential roles in normal body functions and metabolic activities. However, few methods have simultaneously measured all nine water-soluble vitamins in biological matrices. In this study, we developed a sensitive and accurate method for the simultaneous measurement of thiamine (B1), riboflavin (B2), nicotinamide (B3), pantothenic acid (B5), 4pyridoxic acid (B6), biotin (B7), 5-methyltetrahydrofolic acid (B9), ascorbic acid (VC), and methylmalonic acid (MMA) in human serum. Samples were analyzed using ultrahigh-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) with Waters HSS T 3 and BEH C 18 columns, each measuring 2.1 × 50 mm with a particle size of 1.7 μm. The mass spectrometer was equipped with an electrospray ionization source, and optimized multiple reaction monitoring was employed to detect all compounds in positive ionization mode. The capillary voltage was set at 0.5 kV. Nitrogen was used as the desolvation gas, with a flow rate of 1000 L/h at 500 °C. Argon was used as the collision gas. The method's performance was validated according to the Clinical and Laboratory Standards Institute guidelines assessing linearity, limit of quantitation, precision, accuracy, carryover, matrix effects, recovery, and dilution. The results confirmed the successful validation of this method for water-soluble vitamins in serum. However, the presence of common endogenous interferences and the type of blood collection tube influenced the results for certain water-soluble vitamins. The results showed that this method was satisfactory, offering significant improvements in analytical performance, including shorter analysis time, higher sensitivity, and the requirement of a smaller sample volume.

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Detection and quantification of vitamins in microliter volumes of biological samples by LC‐MS for clinical screening

Cited by 4 publications

References 42 publications

Herbivory meets fungivory: insect herbivores feed on plant pathogenic fungi for their own benefit

Herbivory meets fungivory: insect herbivores feed on plant pathogenic fungi for their own benefit

Dilute-and-shoot-based direct nano-electrospray ionization tandem mass spectrometry as screening methodology for multivitamins in dietary supplement and human urine

Rapid Determination of Water-Soluble Vitamins in Human Serum by Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry

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