Detection and quantitation by lysis-filtration of bacteremia after different oral surgical procedures

Heimdahl, Anders; Hall, G.; Hedberg, Maria; Sandberg, Hans; Söder, Per‐Östen; Tunér, K; Ce, Nord

doi:10.1128/jcm.28.10.2205-2209.1990

Cited by 241 publications

(177 citation statements)

References 16 publications

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“…A number of studies have estimated the type, frequency and extent of post-surgical bacteremia (1-9, 14-24, 26 32). The incidence of dental bacteremia varies from one study to another (17-100%) (2,32), and the interpretation and comparison of the results are difficult since patient selection and especially, the microbiological techniques used to isolate and identify the microorganisms, are different. Recently developed methods for isolation of microorganisms from the blood stream, combining anaerobic culture techniques and filtration of blood have brought renewed attention to this field (32).…”

mentioning

confidence: 99%

“…The incidence of dental bacteremia varies from one study to another (17-100%) (2,32), and the interpretation and comparison of the results are difficult since patient selection and especially, the microbiological techniques used to isolate and identify the microorganisms, are different. Recently developed methods for isolation of microorganisms from the blood stream, combining anaerobic culture techniques and filtration of blood have brought renewed attention to this field (32). The purpose of the present study was to investigate the incidence of bacteremia following endodontic treatment of teeth with apical periodontitis, and to estimate if these microorganisms had the root canal as the source of infection.…”

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Bacteremia in conjunction with endodontic therapy

Debelian

Olsen

Tronstad

1995

Dental Traumatology

137

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This study characterizes oral microorganisms believed to have spread from the root canal into the blood stream during and after endodontic therapy of teeth with Asymptomatic apical periodontitis. Microbiological samples were taken under aseptic conditions from the root canal of 26 single-rooted teeth in 26 patients. In the endodontic treatment of 13 of the patients (Group 1), the first 3 reamers, sizes 15, 20 and 25, were used to a level 2 mm beyond the apical foramen. In the other 13 patients (Group 2), the instrumentation ended inside the root canal 1 mm short of the apical foramen. Blood samples were taken from the patients during the instrumentation and 10 min after the treatment was completed. Anaerobic microorganisms were isolated from all root canals. In 7 patients of Group 1, Propionibacterium acnes, Peptostreptococcus prevotii, Fusobacterium nucleatum, Prevotella intermedia and Saccharomyces cerevisiae were recovered from the blood. In 4 patients of Group 2, P. intermedia, Actinomyces israelii, Streptococcus intermedius and Streptococcus sanguis were isolated from the blood. Biochemical tests and antibiograms revealed that the isolates from the root canal and blood had identical profiles within the patients, strongly suggesting that the microorganisms isolated from the blood had the root canal as their source.

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confidence: 99%

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confidence: 99%

Bacteremia in conjunction with endodontic therapy

Debelian

Olsen

Tronstad

1995

Dental Traumatology

137

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“…A number of studies have estimated the type, frequency and extent of post-surgical bacteremia (4)(5)(6)(7)(8)(9)(10)(11)(12)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34). The incidence of dental bacteremia varies from one study to another from 17% to 100% (4, 34).…”

Section: Incidence Of Bacteremiamentioning

confidence: 99%

“…With this technique, blood is drawn from a patient's cubital vein by means of Vacutainer tubes that contain a 0.35% solution of sodium polyanaetholesulfonate (SPS). The blood collected is diluted 1:20 in a lysing solution, and then filtered through a cellulose membrane (33)(34)(35)(36). The lysis solution contains a commercial streptokinase-streptodornase compound to avoid clogging of the filters during filtration of the blood (29).…”

Section: Incidence Of Bacteremiamentioning

confidence: 99%

“…Moreover, bacteremia after dental extraction, third molar surgery, dental scaling, endodontic treatment and bilateral tonsillectomy has been studied by means of lysis-filtration of blood samples with subsequent aerobic and anaerobic incubation (34). Bacteremia was observed in 100% of the patients after dental extraction, in 55% after thirdmolar surgery, in 70%) after dental scaling, in 20% after endodontic treatment, and in 55% after bilateral tonsillectomy.…”

Section: Microbiology Of Transient Bacteremiamentioning

confidence: 99%

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Systemic diseases caused by oral microorganisms

Debelian¹,

Olsen²,

Tronstad³

1994

Dental Traumatology

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Human endodontic and periodontal infections are associated with complex microfloras in which approximately 150, (in apical periodontitis) and 350 (in marginal periodontitis) bacterial species have been encountered. These infections are predominantly anaerobic, with gram-negative rods being the most common isolates. The anatomic closeness of this microflora to the bloodstream can facilitate bacteremia and systemic spread of bacterial by-products and immunocomplexes. A variety of clinical procedures such as tooth extraction, periodontal and endodontic treatment, may cause translocation of microorganisms from the oral cavity to the bloodstream. The microorganisms that gain entrance to the blood circulate throughout the body, but are usually eliminated by the host (reticuloendothelial system) within minutes. However, in patients with ineffective heart valves or vascular diseases, bacteremia can be a potential danger, leading most commonly to infective endocarditis and myocardial or cerebral infarction. Other forms of systemic diseases such as brain abscesses, hematological infections and implant infections have also been related to oral microorganisms.

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Periodontal Infections and Coronary Heart Disease

Spahr

Klein²,

Khuseyinova³

et al. 2006

Arch Intern Med

216

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Background: Chronic inflammation from any source is associated with increased cardiovascular risk. Periodontitis is a possible trigger of chronic inflammation. We investigated the possible association between periodontitis and coronary heart disease (CHD), focusing on microbiological aspects.Methods: A total of 789 subjects (263 patients with angiographically confirmed, stable CHD and 526 populationbased, age-and sex-matched controls without a history of CHD) were included in the Coronary Event and Periodontal Disease (CORODONT) study. Subgingival biofilm samples were analyzed for periodontal pathogens Actinobacillus actinomycetemcomitans, Tannerella forsythensis, Porphyromonas gingivalis, Prevotella intermedia, and Treponema denticola using DNA-DNA hybridization. The need for periodontal treatment in each subject was assessed using the Community Periodontal Index of Treatment Needs (CPITN). The main outcome measures included total periodontal pathogen burden, number of the vari-ous periodontal pathogens in the subgingival biofilm, and periodontal treatment needs (according to the CPITN).Results: In multivariable analyses, we found a statistically significant association between the periodontal pathogen burden (log 10 of the sum of all pathogens) (odds ratio [OR], 1.92; 95% confidence interval [CI], 1.34-2.74; PϽ.001) or the number of A actinomycetemcomitans in periodontal pockets (log 10 ) (OR, 2.70; 95% CI, 1.79-4.07; PϽ.001) and the presence of CHD. In addition, a statistically significant association between an increase in mean CPITN score by 1 and the presence of CHD (OR, 1.67; 95% CI, 1.08-2.58; P =.02) was observed.Conclusions: Our findings suggest an association between periodontitis and presence of CHD. Periodontal pathogen burden, and particularly infection with A actinomycetemcomitans, may be of special importance.

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Detection and quantitation by lysis-filtration of bacteremia after different oral surgical procedures

Cited by 241 publications

References 16 publications

Bacteremia in conjunction with endodontic therapy

Bacteremia in conjunction with endodontic therapy

Systemic diseases caused by oral microorganisms

Periodontal Infections and Coronary Heart Disease

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