Detection and quantitation of a bioactive compound inVicia narbonensis L. seeds by capillary electrophoresis-mass spectrometry: A comparative study with UV detection

Abstract

Capillary zone electrophoresis with mass spectrometry (CE-MS) and UV detection (CE-UV) was applied to the quantitative determination of gamma-glutamyl-S-ethenyl-cysteine (GEC), a bioactive and unstable compound present in Vicia narbonensis L. seeds. This compound is responsible for, among other negative effects, palatability reduction and grain toxicity. In order to carry out the quantitative analysis of GEC, different conditions (such as composition, concentration and pH of the background electrolyte, and type and time of extraction) were studied. Also, adequate conditions for electrospray-mass spectrometry of this bioactive compound were investigated. The best extraction conditions of GEC from V. narbonensis L. seeds flour were obtained using ethanol-water (70:30 v/v) for 45 min. The use of a 20 m ammonium hydrogen carbonate at pH 7 provided adequate analytical conditions compatible with the unstable nature of GEC as well as with the requirements of CE-UV and CE-MS analysis. A comparative study was carried out between the different figures of merit of CE-UV and CE-MS for quantitative purposes. Both techniques provided similar limit of detection and can be applied with confidence within the same linear dynamic range. However, reproducibility and speed of analysis were better using CE-UV. The developed methods were readily applied to quantify GEC in seeds of 21 genotypes of V. narbonensis L. A good agreement between CE-MS and CE-UV results was observed corroborating the usefulness of both approaches for quantitative purposes.

Identification and quantitation of cis-ketoconazole impurity by capillary zone electrophoresis–mass spectrometry

“…Nevertheless, RSD values obtained for corrected peak areas were much worse for MS detection than for UV detection. Although these values seem to be very high (see Table 1), similar values have been reported in other works on CE-MS published recently [22]. Finally, the quantitation of the impurity in the different samples analyzed was performed.…”

Biochemical characterization of legume seeds as ingredients in animal feed

“…Table 4 shows its content in the two cultivars of V. narbonensis. Arias et al (2005) found that its content in the seeds ranges between 15 and 31 mg/g in 21 V. narbonensis cultivars collected in different Spanish and Italian regions. Castleman (2000) found GEC in a range of 4.1 to 37.7 mg/g in accordance with the results obtained in this work (table 4).…”

Recent advances in the application of capillary electromigration methods for food analysis

“…the use of CE to analyze proteins from milk and dairy products [33][34][35][36][37][38][39][40][41], the second one would cover the analysis of proteins from cereals (mainly wheat proteins) [42][43][44][45][46][47][48][49], and the third one would include all the other applications [49][50][51][52][53][54][55].…”

“…This includes the detection and quantitation of a bioactive peptide in Vicia narbonensis L. seeds [51], the characterization of proteins from Spirulina platensis microalga using CE-ion trap-mass spectrometry and CE-time of flight-mass spectrometry [52], the CE-MS analysis of basic proteins in foods using a new physically adsorbed polymer coating [53] and the investigation of peptidic maps from proteins using CE-MS and a peptide modelling [54].…”

“…Gliadins Wheat (Triticum species) CE-UV using acidic or isoelectric buffers [49] Proteins Cider CGE-UV using linear polyacrylamide [50] Bioactive peptide Vicia narbonensis CE-UV and CE-MS with ESI compatible buffer [51] …”