Detection and quantitation of curcumin in mouse lung cell cultures by matrix-assisted laser desorption ionization time of flight mass spectrometry

May, Lisa; Tourkina, Elena; Hoffman, Stanley; Dix, Thomas A.

doi:10.1016/j.ab.2004.10.037

Cited by 28 publications

(14 citation statements)

References 27 publications

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“…Untreated and curcumin-treated mouse lung fibrotic and nonfibrotic cell cultures were analysed by MALDI and curcumin uptake was calculated. Curcumin was not detected in untreated cells [62].…”

Section: Quantification Of Other Compounds By Maldimentioning

confidence: 84%

Quantitative matrix-assisted laser desorption/ionization mass spectrometry

Duncan¹,

Röder²,

Hunsucker³

2008

Briefings in Functional Genomics and Proteomics

197

162

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This review summarizes the essential characteristics of matrix-assisted laser desorption/ionization (MALDI) timeof-flight mass spectrometry (TOF MS), especially as they relate to its applications in quantitative analysis. Approaches to quantification by MALDI-TOF MS are presented and published applications are critically reviewed.Keywords: quantification; quantitative analysis; MALDI; mass spectrometry; biomarkers OVERVIEWSince its inception in 1987 [1], matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry (TOF MS) has been applied for the analysis of a wide range of biomolecules. Initial applications were almost exclusively for the qualitative analysis of biopolymers because MALDI-TOF MS provided a fast and accurate approach to molecular mass and purity information: Is my material the right mass and is it free from contaminants? Because of the speed of analysis, ease of use, relative low equipment cost, ease of data interpretation and limited potential for cross-contamination between samples/users, MALDI-TOF MS systems were considered walk-up instruments where investigators run their own samples and determine, within minutes, whether they were on the right track with their work.There were, however, two specific areas of application where MALDI-TOF MS was initially viewed as impractical: i.e. the analysis of low mass analytes and quantitative applications. Low mass analysis is complicated by the vast molar excess of the matrix that can swamp any analyte-specific signal in the low m/z region of the spectrum. Quantitative analysis was viewed as implausible because crystallization does not yield a uniform distribution of the analyte and matrix across the target surface and this gives rise to regions where the analyte signal is especially intense relative to other locations on the target surface. MALDI MS was therefore viewed as inherently irreproducible because, for a given amount of analyte loaded onto the target, the measured ion intensity varies.Subsequently, it has been shown that both these perceived limitations can be overcome and quantification can be routine, both for low and high mass analytes. Now, an extensive list of published applications includes quantification of amino acids, lipids, natural products, drugs, polymers, herbicides, metabolites, toxins, oligonucleotides, carbohydrates, peptides and proteins. (Selected applications from these areas are discussed at the end of this review.) Here, we focus on the quantitative applications of MALDI and illustrate applications relating to both low and high-molecular mass analytes. We also discuss the strategies for applying MALDI to relative and absolute quantification. While MALDI is most commonly combined with TOF analysers, other analyser options are possible and applications employing these are also presented. Our aim is not to provide an exhaustive catalogue of published applications, but to discuss the general principles and to illustrate the

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Section: Quantification Of Other Compounds By Maldimentioning

confidence: 84%

Quantitative matrix-assisted laser desorption/ionization mass spectrometry

Duncan¹,

Röder²,

Hunsucker³

2008

Briefings in Functional Genomics and Proteomics

197

162

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“…It has been reported that curcumin has many pharmacological functions, such as antioxygenation, antibiosis and antitumor [1][2][3] . Methods such as high-performance thin-layer chromatographic (HPTLC) [4][5][6][7] , high-performance liquid chromatography (HPLC) [8][9][10][11] , capillary electrophoresis with amperometric detection (CE-AD) 12 , matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOFMS) 13 , spectrofluorimetry 14,15 , liquid phase tandem mass spectrometry (LC-MS/MS) 16,17 , 1 H NMR 17 , resonance light scattering technique (RLS) 18 and spectrophotometry [19][20][21] have been employed to determine curcumin in a variety of matrixes. However, most of these methods have low sensitivity, need a large number of organic solvents or metal ions.…”

Section: Curcuminmentioning

confidence: 99%

High Sensitivity Method for Determination of Trace Curcumin in the Aqueous Phase

Chang

Chen

et al. 2014

J. Chil. Chem. Soc.

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A new ionic liquid (IL) based micro-extraction method was developed for the spectrophotometry and spectrofluorometry determination of curcumin in curry products. ILs can effectively extract curcumin from aqueous solution which were used instead of large-scale organic solvents to reduce environmental pollution and production cost. Results show that the fluorescence of curcumin is considerably enhanced in 1-hexyl-3-methylimidazolium hexafluorophosphate ([Hmim][PF 6 ]). Under optimized conditions, the absorbance of curcumin is proportional to the concentration of curcumin in the range of 18.4 ng/mL to 494 ng/mL, and the detection limit is 6.1 ng/mL. In addition, the fluorescence intensity of curcumin is proportional to the concentration of curcumin in the range of 0.050 ng/mL to 120.1 ng/mL, and the detection limit is 0.002 ng/mL. Therefore, an efficient spectrophotometry and spectrofluorometry method for the determination of curcumin was established, and the actual concentration in curry products was satisfactorily determined. The content of curcumin in curry sauce were 14.10±0.40 and 14.32±0.37 mg/g when using the spectrophotometry and spectrofluorometry, the content of curcumin in curry powder were 2.50±1.73 and 2.75±1.40 mg/g when using the spectrophotometry and spectrofluorometry.

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“…Yang et al (2007) developed another LC-MS/ MS method to study oral bioavailability of curcumin in rat with a LLOQ of 5 ng/mL and a run time of 5 min. Still another paper described a method based on matrix-assisted laser desorption ionization time-of-flight mass spectrometry for the study of curcumin and its metabolites in mouse serum and mouse lung cell cultures (May et al, 2005). Although LC-MS/MS method can provide excellent sensitivity and short run time, the apparatus is expensive and the matrix effects are difficult to overcome.…”

Section: Introductionmentioning

confidence: 99%

A rapid and simple HPLC method for the determination of curcumin in rat plasma: assay development, validation and application to a pharmacokinetic study of curcumin liposome

Jiang

Wen

et al. 2009

Biomedical Chromatography

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This paper describes a sensitive, specific and rapid high-performance liquid chromatography (HPLC) method for the determination of curcumin in rat plasma. After a simple step of protein precipitation in 96-well format using acetonitrile containing the internal standard (IS), emodin, plasma samples were analyzed by reverse-phase HPLC. Curcumin and the IS emodin were separated on a Diamonsil C(18) analytical column (4.6 x 100 mm, 5 microm) using acetonitrile-5% acetic acid (75:25, v/v) as mobile phase at a flow rate of 1.0 mL/min. The method was sensitive with a lower limit of quantitation of 1 ng/mL, with good linearity (r(2) >or= 0.999) over the linear range 1-500 ng/mL. All the validation data, such as accuracy and precision, were within the required limits. A run time of 3.0 min for each sample made high-throughput bioanalysis possible. The assay method was successfully applied to the study of the pharmacokinetics of curcumin liposome in rats.

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Detection and quantitation of curcumin in mouse lung cell cultures by matrix-assisted laser desorption ionization time of flight mass spectrometry

Cited by 28 publications

References 27 publications

Quantitative matrix-assisted laser desorption/ionization mass spectrometry

Quantitative matrix-assisted laser desorption/ionization mass spectrometry

High Sensitivity Method for Determination of Trace Curcumin in the Aqueous Phase

A rapid and simple HPLC method for the determination of curcumin in rat plasma: assay development, validation and application to a pharmacokinetic study of curcumin liposome

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