Detection and Quantitation of the Explosives 2,4,6-Trinitrotoluene and Hexahydro-1,3,5-trinitro-1,3,5-triazine in Groundwater Using a Continuous Flow Immunosensor

Bart, John C.; Judd, Linda L.; Hoffman, Karen E.; Wilkins, Angela M.; Charles, Paul T.; Kusterbeck, Anne W.

doi:10.1021/bk-1997-0657.ch017

Cited by 10 publications

(14 citation statements)

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“…Studies in which buffer was spiked with TNT yielded detection limits (defined as three standard deviations above the blank) of 5-50 ppb for the agarose beads [22,33,34], 10 ppb for the membrane based system [25,30,31,35], and 15-20 ppt for the microcapillary [28,29]. Similar results were obtained for RDX [25,28,29,30,31,35].…”

Section: Resultssupporting

confidence: 53%

Non-aerosol detection of explosives with a continuous flow immunosensor

Shriver‐Lake

Charles

Kusterbeck

2003

Analytical and Bioanalytical Chemistry

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Contamination of groundwater, soil, and the marine environment by explosives is a global issue. Identification, characterization and remediation are all required for a site recognized as contaminated with 2,4,6-trinitrotoluene (TNT) or hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX). For each step, a method to accurately measure the contaminant level is needed. This paper reviews some of the current methods with emphasis on a single biosensor developed in our laboratory. Current regulatory methods require samples to be sent off-site to a certified laboratory resulting in time delays up to a month. A continuous flow biosensor for detection of explosives has been developed and tested for the rapid field screening of environmental samples. The detection system is based on a displacement immunoassay in which monoclonal antibodies to (TNT) and RDX are immobilized on solid substrates, allowed to bind fluorescently labeled antigens, and then exposed to explosives in aqueous samples. Explosive compounds present in the sample displace proportional amounts of the fluorescent label, which can then be measured to determine the original TNT or RDX concentration. The system can accurately detect ppb to ppt levels of explosives in groundwater or seawater samples and in extracts of contaminated soil. The biosensor has applications in environmental monitoring at remediation sites or in the location of underwater unexploded ordnance.

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Section: Resultssupporting

confidence: 53%

Non-aerosol detection of explosives with a continuous flow immunosensor

Shriver‐Lake

Charles

Kusterbeck

2003

Analytical and Bioanalytical Chemistry

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“…, and hexahydra-1,3,5-trinitro-triazine (RDX; Radian International). Cy5-TNB and Cy5-RDX were synthesized as described previously (Cy5 labeling kit was purchased from Biological Detection Systems, Pittsburgh, PA) (25). Anti-TNT (anti-TNT/SD) and anti-RDX (anti-RDX/SD) antibodies were purchased from Strategic Diagnostics, Inc. All reagents were used as received without further purification and aqueous solutions were prepared in doubly distilled deionized water unless otherwise noted.…”

mentioning

confidence: 99%

Multianalyte Detection Using a Capillary-Based Flow Immunosensor

Narang

Gauger²,

Kusterbeck

et al. 1998

Analytical Biochemistry

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“…( Refs. 18,19) Our goal for the linear regressions was a slope significantly different from zero with 95% confidence (assessed by t-test). A student's two-tailed paired t-test (a test of accuracy) and the Fisher F-test (a test of variance) at the 95% confidence level were performed on all field trial data values.…”

Section: Performance Objectivesmentioning

confidence: 99%

“…(Ref. 19) For the on-site field trial, 20 gm of soil was mixed with 100 mL acetone. The sample was shaken for three minutes and then filtered.…”

Section: S Oil Extractionmentioning

confidence: 99%

Investigation of Alternative Energetic Compositions for Small Electro-Explosive Devices for Medium Caliber Ammunition

Hirlinger¹,

Cheng²

2004

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Detection and Quantitation of the Explosives 2,4,6-Trinitrotoluene and Hexahydro-1,3,5-trinitro-1,3,5-triazine in Groundwater Using a Continuous Flow Immunosensor

Cited by 10 publications

References 1 publication

Non-aerosol detection of explosives with a continuous flow immunosensor

Non-aerosol detection of explosives with a continuous flow immunosensor

Multianalyte Detection Using a Capillary-Based Flow Immunosensor

Investigation of Alternative Energetic Compositions for Small Electro-Explosive Devices for Medium Caliber Ammunition

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