Detection and quantitation of two cucurbit criniviruses in mixed infection by real-time RT-PCR

Abrahamian, Peter; Seblani, Rewa; Sobh, H.; Abou‐Jawdah, Y.

doi:10.1016/j.jviromet.2013.06.004

Cited by 28 publications

(15 citation statements)

References 41 publications

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“…Moreover, it was reported that CCYV and CYSDV ( Cucurbit yellow stunting disorder virus ) showed mixed infections in Lebanon20. Studies on CCYV were mainly on the detection methods and plant resistance breeding122122, but the details of the transmission process and the virus retention site within its vectors are still unclear20.…”

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confidence: 99%

Direct evidence for the semipersistent transmission of Cucurbit chlorotic yellows virus by a whitefly vector

Liang

Wang

et al. 2016

Sci Rep

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Cucurbit chlorotic yellows virus (CCYV) (genus Crinivirus, family Closteroviridae) is an emerging plant virus, and is now spreading and causing severe economic losses to cucurbit crops in many Asian countries. CCYV is believed to be transmitted specifically by the sweetpotato whitefly, Bemisia tabaci, in a semipersistent manner. In the present study, we provide direct evidence for the semipersistent transmission of CCYV by Mediterranean (MED) cryptic species of B. tabaci complex. We investigated CCYV transmission characteristics, and immunofluorescently labeled and localized the virus retention site within the vector by laser confocal microscopy. Whiteflies required ≥1 h of acquisition access period (AAP) to successfully acquire CCYV, and the proportion of RT-PCR positive whitefly individuals reached to 100% at 48 h of AAP. CCYV virons could be retained within vectors as long as 12 d, but the proportion of RT-PCR positive whiteflies dropped to 55% by 3 d. Groups of thirty whiteflies given a 24 h of inoculation access period (IAP) to inoculate CCYV on cucumber plants showed a transmission efficiency rate of 72.73%. The retention site of CCYV virons was located in the foregut of virion-fed vectors. These results definitely indicated the semipersistent transmission mode of CCYV by B. tabaci MED.

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Direct evidence for the semipersistent transmission of Cucurbit chlorotic yellows virus by a whitefly vector

Liang

Wang

et al. 2016

Sci Rep

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“…Primers based on Hsp70h gene were capable of detecting more (30-50 % higher chance) BPYV samples than those of the CP gene [70]. CCYV and CYSDV may often occur in mixed infections, specific primers were designed to distinguish between the two infections in multiplex RT-PCR [4].…”

Section: Diagnosismentioning

confidence: 99%

Whitefly-transmitted criniviruses of cucurbits: current status and future prospects

Abrahamian

Abou‐Jawdah

2013

VirusDis.

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In the past decade, crinviruses have gained interest due to their rapid widespread and destructive nature for cucurbit cultivation. Several members of the genus Crinivirus are considered emerging viruses. Currently, four criniviruses: Beet pseudo-yellows virus, Cucurbit chlorotic yellows virus, Cucurbit yellow stunting disorder virus and Lettuce infectious yellows virus have been reported to infect field-or greenhouse-grown cucurbits. Apart from their cucurbit hosts, criniviruses infect other cash crops and weeds. Criniviruses are exclusively transmitted by whiteflies. The virion titer and the vector genus or species complex are predominant factors affecting virus transmission. These criniviruses maintain genetic stability with limited intra-species variability. They share similar core genome structure and replication strategies with some variations in the non-core proteins and downstream replication processes. Management of the diseases induced by criniviruses relies on integrated disease management strategies and on resistant varieties, when available. This review will cover their epidemiology, molecular biology, detection and management.

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“…Cucurbits are known to be infected by a wide range of viruses causing significant crop losses in both quality and quantity. The most important pathogens of these viruses are A c c e p t e d M a n u s c r i p t (SqMV), Tobacco ring spot virus (TRSV) (Lecoq et al 2003), Cucurbit chlorotic yellows virus (CCYV) and Cucurbit yellow stunting disorder virus (CYSDV) (Abrahamian et al 2013). …”

Section: Introductionmentioning

confidence: 99%

Resistance induction in pumpkinCucurbita maximaL. againstWatermelon mosaic potyvirusby plant growth-promoting rhizobacteria

Elbeshehy

Youssef

Elazzazy

2015

Biocontrol Science and Technology

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Detection and quantitation of two cucurbit criniviruses in mixed infection by real-time RT-PCR

Cited by 28 publications

References 41 publications

Direct evidence for the semipersistent transmission of Cucurbit chlorotic yellows virus by a whitefly vector

Direct evidence for the semipersistent transmission of Cucurbit chlorotic yellows virus by a whitefly vector

Whitefly-transmitted criniviruses of cucurbits: current status and future prospects

Resistance induction in pumpkinCucurbita maximaL. againstWatermelon mosaic potyvirusby plant growth-promoting rhizobacteria

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