Detection and sequencing of rotavirus VP7 gene from human materials (stools, sera, cerebrospinal fluids, and throat swabs) by reverse transcription and PCR

Ushijima, Hiroshi; Xin, Ke-Qin; Nishimura, Shigeyuki; Morikawa, Shigeki; Abe, Toshiaki

doi:10.1128/jcm.32.12.2893-2897.1994

Cited by 56 publications

(18 citation statements)

References 15 publications

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“…GE‐associated seizure is more commonly seen in Southeast Asia and is seldomly reported in North America and Europe 7 . The reported incidence of rotavirus‐associated convulsion was 2.9% from Japan, 8 and 5.7% from local studies 9 . Our study shows a lower incidence of rotavirus‐associated seizure compared with previous studies.…”

Section: Discussioncontrasting

confidence: 47%

Norovirus as cause of benign convulsion associated with gastro‐enteritis

Chan

et al. 2011

J Paediatrics Child Health

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Section: Discussioncontrasting

confidence: 47%

Norovirus as cause of benign convulsion associated with gastro‐enteritis

Chan

et al. 2011

J Paediatrics Child Health

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“…For instance, it was not possible to amplify the VP7 gene from the CSF. This was likely due to the low viral load, below the RT‐PCR detection limit, as observed by Ushijima et al [1994]. Similar technical constraints have been reported during genotyping of RV strains [Lynch et al, 2001; Nigrovic et al, 2002] in RV‐positive CSF samples.…”

supporting

confidence: 61%

“…In the VP7 strain PR267/08/M clustered in the phylogenetic sub‐lineage G1‐Ic. This lineage contains an additional three strains detected in CSF samples (C2, C4, and TE2 strains) and one strain (GC06‐01) detected in the stools from children with CNS disease, even if in a different sub‐cluster [Ushijima et al, 1994; Shiihara et al, 2007]. This observation might be a coincidence, but is noteworthy.…”

mentioning

confidence: 99%

Case report: Detection of rotavirus RNA in the cerebrospinal fluid of a child with rotavirus gastroenteritis and meningism

Medici

Abelli

Guerra

et al. 2011

Journal of Medical Virology

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Although case reports have described detection of rotavirus (RV) in extraintestinal sites such as the liver, kidney, and central nervous system (CNS) of children with RV gastroenteritis, CNS localization in RV infection seems to be rare. RT‐PCR and nucleotide sequencing detected a G1P[8] strain in the stool and cerebrospinal fluid (CSF) samples of a patient with concurrent RV‐associated enteritis and CNS signs. Upon sequence analysis, the viruses detected in the CSF was identical to the virus detected in the stools. In the VP7‐ and VP4‐based phylogenetic dendograms the strain clustered within the G1‐Ic sub‐lineage and the P[8]‐III lineage. This study supports the hypothesis that RV infection was able to spread from the intestinal tract to the CNS, and likely played a role in the onset of neurological disease. J. Med. Virol. 83:1637–1640, 2011. © 2011 Wiley‐Liss, Inc.

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“…All untypable and mixed-serotypeable specimens by ELISA were G-typed by RT-PCR with specific primers. Rotavirus double-stranded RNA was extracted by the phenol-chloroform and glass powder methods as previously described [Ushijima et al, , 1994. G genotyping was undertaken in two steps: first and second amplification, and was carried out using a nested RT-PCR as described previously [Gouvea et al, 1990] with a minimal change in the cycling condition in 35 cycles (948C for 1 min, 458C for 2 min, 728C for 3min, and a final extension 7 min at 728C).…”

Section: G and P Typing By Rt-pcrmentioning

confidence: 99%

Epidemiological features of rotavirus infection among hospitalized children with gastroenteristis in Ho Chi Minh City, Vietnam

Doan

Okitsu

Nishio

et al. 2003

Journal of Medical Virology

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An epidemiological study of the G serotype and P genotype distribution of group A rotaviruses by using ELISA and/or RT-PCR was conducted in children (aged 1 month to 15 years) with diarrhea that were admitted to the General Children's Hospital No. 1, Ho Chi Minh City, Vietnam from December 1999 to November 2000. The results showed that rotavirus is associated with 65.6% (889/1355) of diarrheal admissions. Rotavirus infection mostly affected children under 2 years of age with a peak incidence in children 1 to 2 years of age (75.7%) and it occurs year round with a slight seasonal pattern; 99.5% of the specimens could be G-typed: G1 was predominant (68.7%), followed by G4 (15.4%), G2 (12.3%), G3 (0.6%), and G9 (0.5%). High identities of VP7 nucleotide (96.3 to 96.9%) and deduced amino acid (98.1 to 98.4%) were found between two Vietnamese G9 strains and also the recent emergence of G9 strains US 1205, Brazilian R143, and Malawian MW69. Mixed infections were identified in 17 (2.0%), and 5 strains (0.5%) remained untypable. The four most common worldwide strains, G1P[8], G2P[4], G3P[8], and G4P[8], constituted 81.1% of all rotaviruses typed with G1P[8] being the most prevalent type (58.2%). Unusual G/P combinations (11 strains) were detected in 11.7% of all strains, of which, G1P[4] was the most prevalent, accounting for 5.6% of the total. Several combinations of G and P types were observed in this study, suggesting a complex rotavirus infection pattern in Vietnam. This study has provided for the first time clear indication on the circulating G and P genotypes among hospitalized children in Ho Chi Minh City, Vietnam. The results suggest that these viral infections are prevalent among hospitalized children and that the four most common worldwide G types as well as the four most common G-P combinations were also infecting children in Ho Chi Minh City, Vietnam. This result could have important implications for rotavirus vaccine programs and for understanding the epidemiological characteristics of human rotavirus in Ho Chi Minh City, Vietnam.

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Detection and sequencing of rotavirus VP7 gene from human materials (stools, sera, cerebrospinal fluids, and throat swabs) by reverse transcription and PCR

Cited by 56 publications

References 15 publications

Norovirus as cause of benign convulsion associated with gastro‐enteritis

Norovirus as cause of benign convulsion associated with gastro‐enteritis

Case report: Detection of rotavirus RNA in the cerebrospinal fluid of a child with rotavirus gastroenteritis and meningism

Epidemiological features of rotavirus infection among hospitalized children with gastroenteristis in Ho Chi Minh City, Vietnam

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