Detection and typing of<i>Clostridium perfringens</i>from retail chicken meat parts

Güran, Hüsnü Şahan; Öksüztepe, Gülsüm

doi:10.1111/lam.12088

Cited by 38 publications

(25 citation statements)

References 29 publications

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“…Isolates carrying both the gene i.e. cpa and cpb2 were recorded in the present study, the findings of which was similar to the findings of Guran et al (2013) and Datta et al (2014) who found cpa and cpb2 with 2.1 percent and 33.3 percent prevalence rate, respectively. Toxin cpb (betatoxin) is a highly trypsin-sensitive protein that causes mucosal necrosis and possibly central nervous signs observed in domestic animals.…”

Section: Toxinotyping Of C Perfringenssupporting

confidence: 91%

Toxinotyping of Clostridium perfringens in Poultry from Necrotic Enteritis Cases

Shelke¹

2020

JAR

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Clostridium perfringens is well known causative agent of necrotic enteritis in poultry and is mainly caused by Type A toxin. NetB toxin is found to be one of the newly emerging virulent toxin gene which is also responsible for necrotic enteritis. The present study was carried out to characterize and to type the different toxins associated with C. perfringens in NE cases of poultry. For the present study total 125 samples were collected from poultry birds, out of which 50 samples were of intestinal content from diseased birds, 50 cloacal swabs and 25 intestinal content from healthy birds. These samples were further processed for isolation, identification, and toxinotyping of Clostridium perfringens isolates. Onisolation of C. perfringens on blood agar total 43 isolates were found positive showing a pattern of double hemolysis on blood agar. The positive isolates of C. perfringens were further confirmed by using 16S rRNA species specific PCR. After confirmation isolates were processed for toxinotyping mainly targeting cpa, cpb and cpb2 toxins by using multiplex PCR. On toxinotyping it was found that NE in poultry birds were mainly caused by C. perfringens type A. On virulent gene detection of netB toxin, total 4 isolates were found positive for netB toxin. This study pointed out that C. perfringens type A is responsible for development of NE in poultry along with net B toxin which is a new key virulent factor. Further studies of netB toxoid and C. perfringens type A for vaccine production could minimize the clostridial problems in broiler farms.

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Section: Toxinotyping Of C Perfringenssupporting

confidence: 91%

Toxinotyping of Clostridium perfringens in Poultry from Necrotic Enteritis Cases

Shelke¹

2020

JAR

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“…C. perfringens types prevalent in poultry meat and meat parts conducted in Turkey were similar to those found in our study (8,11,15). Although there are no published data on detection of C. perfringens in poultry edible offal samples, previous studies in Turkey showed that edible offal of broiler can be contaminated with different foodborne pathogens mainly including Salmonella spp.…”

Section: Discussionsupporting

confidence: 74%

“…Also Guran and Oksuztepe (2013) reported that 154 of 200 (77%) chicken meat part samples were found to be contaminated with C. perfringens and one isolate was carrying cpe gene. On the other hand, similar to our results, 5% of the isolates were cpb2 positive in that study.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and molecular typing of Clostridium perfringens isolates from edible offal of broiler

GÖNCÜOĞLU¹

2015

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:The aim of the study was to determine the prevalance and molecular characterization of Clostridium perfringens in edible offal of broiler. For this purpose a total of 90 samples, including 45 heart-livers and 45 gizzards were obtained from different supermarkets and transported to the laboratory in an ice box and tested at the same day. Culture technique was used for the isolation and selected biochemical tests, including acid phosphatase and reverse-CAMP tests was used for the identification of C. perfringens from the samples. Twenty one (46.7%) of 45 heart-liver and eight (17.7%) of 45 gizzards were found to be contaminated with C. perfringens. Isolates were molecular characterized by using multiplex PCR with alpha (cpa), beta (cpb), beta 2 (cpb2), epsilon (etx), iota (iA) and enterotoxin (cpe) genes. Results showed that all isolates were carrying cpa gene and named as type A but none of them were positive for cpe gene. Only two gizzard isolates were positive for cpb2 gene. Our results indicate that edible offal of broiler are highly contaminated with C. perfringens type A which is mostly responsible for the foodborne poisoning worldwide. According to the high contamination rate edible offal should be considered as one of the important causes of C. perfringens type A foodborne disease.Key words: Broiler edible offal, Clostridium perfringens, molecular typing, multiplex PCR. Yenilebilir piliç iç organlarında Clostridium perfringens prevalansı ve moleküler tiplendirilmesiÖzet: Bu çalışmada yenilebilir piliç iç organlarında Clostridium perfringens prevalansı ve elde edilen izolatların moleküler karakterizasyonlarının belirlenmesi amaçlanmıştır. Çalışmada farklı süpermarketlerde satışa sunulan 45 kalp-karaciğer ve 45 taşlık olmak üzere toplam 90 yenilebilir iç organ soğuk zincir altında laboratuvara getirilerek aynı gün içerisinde analize alınmıştır. Örneklerden C. perfringens izolasyon ve identifikasyonunda klasik kültür tekniği ile asit fosfataz ve reverse-CAMP gibi biyokimyasal testler kullanılmıştır. Kırk beş kalp-karaciğer örneğinin 21'inin (% 46.7) ve 45 taşlık örneğinin sekizinin (% 17.7) C. perfringens ile kontamine olduğu saptanmıştır. Elde edilen izolatların alfa (cpa), beta (cpb), beta 2 (cpb2), epsilon (etx), iota (iA) ve enterotoksin (cpe) genlerine ait primer dizilimleri kullanılarak multipleks PCR ile moleküler tiplendirilmeleri yapılmıştır. Sonuçlar incelendiğinde bütün izolatların cpa geni taşıdığı belirlenmiş ve tip A olarak sınıflandırılmıştır, bununla beraber hiçbir izolatın cpe genine sahip olmadığı saptanmıştır. İki farklı taşlık örneğinden elde edilen izolatların cpa yanında cpb2 genini de taşıdıkları belirlenmiştir. Sonuç olarak yenilebilir piliç iç organlarının dünya genelinde gıda zehirlenmelerinde en sıklıkla rastlanan C. perfringens tip A ile yüksek oranda kontamine olduğu belirlenmiştir. Yüksek kontaminasyona bağlı olarak yenilebilir piliç iç organlarının C. perfringens tip A zehirlenmeleri açısından önemli birer kaynak oldukları saptanmıştır. Anahtar sözcükler: Clostridium per...

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“…Table 3 shows that out of 32 C. perfringens strains, six of enterotoxigenic strains belonged to type A, two to type D, and one to type E. This is similar to observations of Khan et al . ( 16 ) who toxinotyped six C. perfringens isolates from raw chicken meat and found that three of them were of type A and one each of the remaining three belonged to types B, C, and D. Likewise, the recognition of enterotoxin genes in type A isolates collected from raw and processed chicken meat products was reported by Guran and Oksuztepe ( 13 ). Moreover, the prevalence of C. perfringens exotoxin and enterotoxin was not related to the type of product (P > 0.05) ( Tables 2 and 3 ).…”

Section: Discussionmentioning

confidence: 85%

“…In our study, the occurrence of C. perfringens may be possibly attributed to the method adapted in preparing chicken meat products. Moreover, the occurrence of C. perfringens may be ascribed to a high protein content in sampled products ( 13 ).…”

Section: Discussionmentioning

confidence: 99%

Toxinotyping and antimicrobial resistance of Clostridium perfringens isolated from processed chicken meat products

Hamza

Dorgham

Hakim

2017

Journal of Veterinary Research

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IntroductionThe toxinotyping and antimicrobial susceptibility of Clostridium perfringens strains isolated from processed chicken meat were determined.Material and MethodsTwo hundred processed chicken meat samples from luncheon meats, nuggets, burgers, and sausages were screened for Clostridium perfringens by multiplex PCR assay for the presence of alpha (cpa), beta (cpb), epsilon (etx), iota (ia), and enterotoxin toxin (cpe) genes. The C. perfringens isolates were examined in vitro against eight antibiotics (streptomycin, amoxicillin, ampicillin, ciprofloxacin, lincomycin, cefotaxime, rifampicin, and trimethoprim-sulfamethoxazole)ResultsAn overall of 32 C. perfringens strains (16%) were isolated from 200 processed chicken meat samples tested. The prevalence of C. perfringens was significantly dependent on the type of toxin genes detected (P = 0.0), being the highest in sausages (32%), followed by luncheon meats (24%), burgers (6%), and nuggets (2%). C. perfringens type A was the most frequently present toxinotype (24/32; 75%), followed by type D (21.9 %) and type E (3.1%). Of the 32 C. perfringens strains tested, only 9 (28%) were enterotoxin gene carriers, with most representing type A (n = 6). C. perfringens strains differed in their resistance/susceptibility to commonly used antibiotics. Most of the strains tested were sensitive to ampicillin (97%) and amoxicillin (94%), with 100% of the strains being resistant to streptomycin and lincomycin. It is noteworthy that the nine isolates with enterotoxigenic potential had a higher resistance than the non-enterotoxigenic ones. ConclusionThe considerably high C. perfringens isolation rates from processed chicken meat samples and resistance to some of the commonly used antibiotics indicate a potential public health risk. Recent information about the isolation of enterotoxigenic C. perfringens type E from chicken sausage has been reported.

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Detection and typing ofClostridium perfringensfrom retail chicken meat parts

Cited by 38 publications

References 29 publications

Toxinotyping of Clostridium perfringens in Poultry from Necrotic Enteritis Cases

Toxinotyping of Clostridium perfringens in Poultry from Necrotic Enteritis Cases

Prevalence and molecular typing of Clostridium perfringens isolates from edible offal of broiler

Toxinotyping and antimicrobial resistance of Clostridium perfringens isolated from processed chicken meat products

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